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Keywords: Yeast vacuole
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Journal Articles
J Cell Sci (2017) 130 (9): 1625–1636.
Published: 01 May 2017
... domain of recombinant Ppn2 failed, as the protein remained insoluble. Therefore, we decided to investigate its activity using protein immunoprecipitated from yeast vacuoles. We overexpressed Ppn2 with an N-terminal 3HA tag in yeast, using the strong GPD1 promoter (P GPD1 -3HA-Ppn2). The protein...
Includes: Supplementary data
Journal Articles
J Cell Sci (2014) 127 (23): 5093–5104.
Published: 01 December 2014
.... , Laboratory   C. S. H. ( 2000 ). Methods in Yeast Genetics: A Cold Spring Harbor Laboratory Course Manual   Cold Spring Harbor, NY : Cold Spring Harbor Laboratory Press . Cabrera   M. , Ungermann   C. ( 2008 ). Purification and in vitro analysis of yeast vacuoles.   Methods Enzymol.   451...
Includes: Supplementary data
Journal Articles
J Cell Sci (2006) 119 (12): 2477–2485.
Published: 15 June 2006
... fusion. EMBO J. 23 , 45 -53. Dietrich, L. E., Lagrassa, T. J., Rohde, J., Cristodero, M., Meiringer, C. T. and Ungermann, C. ( 2005 ). ATP-independent control of Vac8 palmitoylation by a SNARE subcomplex on yeast vacuoles. J. Biol. Chem. 280 , 15348 -15355. Drisdel, R. C. and Green, W. N...
Includes: Supplementary data