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Keywords: Yeast
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Journal Articles
J Cell Sci (2022) 135 (5): jcs259167.
Published: 3 February 2022
... ( Fanchiotti et al., 1998 ). By fluorescence microscopy, we observed that both Sp -GFP and Sp -GlyGFP indeed localized to a perinuclear ring and cortical sub-membrane region – the typical localization of ER proteins in fission yeast ( Fig. 3 C). To determine whether the biosensor could report...
Includes: Supplementary data
Journal Articles
J Cell Sci (2021) 134 (18): jcs258301.
Published: 24 September 2021
...Marius Musielak; Carolin C. Sterk; Felix Schubert; Christian Meyer; Achim Paululat; Jürgen J. Heinisch ABSTRACT Rho5 is the yeast homolog of the human small GTPase Rac1. We characterized the genes encoding Rho5 and the subunits of its dimeric activating guanine-nucleotide-exchange factor (GEF...
Includes: Supplementary data
Journal Articles
J Cell Sci (2021) 134 (11): jcs258338.
Published: 4 June 2021
.... To identify factors that impinge on proteostasis during As(III) exposure, we performed a high-content imaging screen in the budding yeast Saccharomyces cerevisiae . A GFP-tagged version of Hsp104, a molecular chaperone that binds to and disassembles protein aggregates ( Glover and Lindquist, 1998...
Includes: Supplementary data
Journal Articles
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Lipid Biology
J Cell Sci (2022) 135 (5): jcs256529.
Published: 17 May 2021
... in the plasma membrane of the living cells. We show here that a stable micron-scale protein-depleted region is generated in the plasma membrane of yeast mutants lacking phosphatidylserine at high temperatures. We named this region the ‘void zone’. Transmembrane proteins and certain peripheral membrane proteins...
Includes: Supplementary data
Journal Articles
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Autophagy
J Cell Sci (2021) 134 (4): jcs253682.
Published: 26 February 2021
..., the cell is not completely degraded through autophagy, implying the existence of some kind of termination mechanism. In the yeast Saccharomyces cerevisiae , autophagy is terminated after 10–12 h of nitrogen starvation. In this study, we found that termination is mediated by re-phosphorylation of Atg13...
Includes: Supplementary data
Journal Articles
J Cell Sci (2020) 133 (23): jcs252551.
Published: 3 December 2020
... find that in Saccharomyces cerevisiae budding yeast, Cdc48 accumulates during cellular stress at intranuclear protein quality control sites (INQ). We show that Cdc48 function is required to suppress INQ formation under non-stress conditions and to promote recovery following genotoxic stress. Cdc48...
Includes: Supplementary data
Journal Articles
J Cell Sci (2020) 133 (19): jcs247684.
Published: 12 October 2020
... to endocytic cargo. To test this idea, we analyzed the yeast adaptor protein Sla1, which binds membrane proteins harboring the endocytic signal NPFxD via the Sla1 SHD1 domain. Consistently, SHD1 domain point mutations that disrupted NPFxD binding caused a proportional reduction in Sla1–GFP recruitment...
Includes: Supplementary data
Journal Articles
J Cell Sci (2020) 133 (16): jcs246983.
Published: 17 August 2020
...Fei Wu; Rinse de Boer; Arjen M. Krikken; Arman Akşit; Nicola Bordin; Damien P. Devos; Ida J. van der Klei ABSTRACT The yeast Hansenula polymorpha contains four members of the Pex23 family of peroxins, which characteristically contain a DysF domain. Here we show that all four H. polymorpha Pex23...
Includes: Supplementary data
Journal Articles
J Cell Sci (2020) 133 (11): jcs240283.
Published: 11 June 2020
...Julian Chollet; Alexander Dünkler; Anne Bäuerle; Laura Vivero-Pol; Medhanie A. Mulaw; Thomas Gronemeyer; Nils Johnsson ABSTRACT Yeast cells select the position of their new bud at the beginning of each cell cycle. The recruitment of septins to this prospective bud site is one of the critical events...
Includes: Supplementary data
Journal Articles
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Mitochondria
J Cell Sci (2020) 133 (10): jcs237917.
Published: 20 May 2020
... or mitochondrial matrix, which have nearly identical heme trafficking dynamics, potentially supporting a role for heme as a mitochondrial–nuclear retrograde signal. Moreover, we discovered that the heme synthetic enzyme 5-aminolevulinic acid synthase (ALAS, also known as Hem1 in yeast), and GTPases in control...
Includes: Supplementary data
Journal Articles
J Cell Sci (2020) 133 (6): jcs241539.
Published: 30 March 2020
.... Nevertheless, when ER stress is prolonged or severe, cell death may occur, accompanied by production of mitochondrial reactive oxygen species (ROS). Using a yeast model ( Saccharomyces cerevisiae ), we describe an innate, adaptive response to ER stress to increase select mitochondrial proteins, O 2 consumption...
Includes: Supplementary data
Journal Articles
J Cell Sci (2020) 133 (6): jcs237156.
Published: 26 March 2020
... that the yeast ( Saccharomyces cerevisiae ) nucleoporin Nup170, in addition to binding and silencing subtelomeric genes, supports transcription of genes regulated by the SAGA transcriptional activator complex. Specifically, we show that a lower amount of SAGA complex is bound to target genes in the absence...
Includes: Supplementary data
Journal Articles
J Cell Sci (2020) 133 (6): jcs236158.
Published: 19 March 2020
...Lauren Budenholzer; Carolyn Breckel; Christopher M. Hickey; Mark Hochstrasser ABSTRACT The proteasome is an essential regulator of protein homeostasis. In yeast and many mammalian cells, proteasomes strongly concentrate in the nucleus. Sts1 from the yeast Saccharomyces cerevisiae is an essential...
Includes: Supplementary data
Journal Articles
J Cell Sci (2020) 133 (3): jcs237982.
Published: 13 February 2020
...Oliver Glomb; Yehui Wu; Lucia Rieger; Diana Rüthnick; Medhanie A. Mulaw; Nils Johnsson ABSTRACT Owing to the local enrichment of factors that influence its dynamics and organization, the actin cytoskeleton displays different shapes and functions within the same cell. In yeast cells, post-Golgi...
Includes: Supplementary data
Journal Articles
J Cell Sci (2019) 132 (21): jcs231688.
Published: 8 November 2019
... of nutrient shortage. One of these strategies is to solidify the cytoplasm, which was observed in bacteria and yeast cells with acutely interrupted energy production. Here, we describe a different type of cytoplasm solidification fission yeast cells switch to, after having run out of nutrients during multiple...
Includes: Supplementary data
Journal Articles
J Cell Sci (2019) 132 (12): jcs228411.
Published: 24 June 2019
... work is properly attributed. Highlighted Article: Distinct substrate specificities of Lag1 and Lac1, the two yeast ceramide synthases, are revealed, shedding light on their physiological roles. Yeast Saccharomyces cerevisiae Lag1 Lac1 Phytoceramide Dihydroceramide Ceramide...
Includes: Supplementary data
Journal Articles
In collection:
Imaging
J Cell Sci (2019) 132 (12): jcs226928.
Published: 17 June 2019
...M. C. Mangione; Kathleen L. Gould ABSTRACT Animal cells, amoebas and yeast divide using a force-generating, actin- and myosin-based contractile ring or ‘cytokinetic ring’ (CR). Despite intensive research, questions remain about the spatial organization of CR components, the mechanism by which...
Journal Articles
J Cell Sci (2019) 132 (11): jcs232249.
Published: 31 May 2019
...–protein interactions screening in mammalian cells, the implementation of PDB-MS in yeast has not been effective. Here, we report a simple and rapid approach in yeast to effectively screen for proximal and interacting proteins in their natural cellular environment by using TurboID, a recently described...
Includes: Supplementary data
Journal Articles
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Metabolism
J Cell Sci (2019) 132 (8): jcs230649.
Published: 17 April 2019
... QUEEN and monitored the dynamics of ATP with good spatial and temporal resolution in living yeasts. We found stable maintenance of ATP concentration in wild-type yeasts, regardless of carbon sources or cell cycle stages, suggesting that mechanism exists to maintain ATP at a specific concentration. We...
Includes: Supplementary data
Journal Articles
In collection:
Mitochondria
J Cell Sci (2018) 131 (22): jcs219956.
Published: 21 November 2018
... protection in yeast . J. Biol. Chem.   289 , 13259 - 13272 . 10.1074/jbc.M113.542910 Bohovych , I. , Chan , S. S. L. and Khalimonchuk , O. ( 2015a ). Mitochondrial protein quality control: the mechanisms guarding mitochondrial health . Antioxid. Redox Signal.   22 , 977 - 994...
Includes: Supplementary data