...Katherine M. Paine; Kamilla M. E. Laidlaw; Gareth J. O. Evans; Chris MacDonald ABSTRACT The yeast ( Saccharomyces cerevisiae ) plasma membrane (PM) is organised into specific subdomains that regulate surface membrane proteins. Surface transporters actively uptake nutrients in particular regions...

... other functions. Peroxisomal membrane contact sites have obtained little attention compared to those of other cell organelles. However, recent studies resulted in a big leap in our knowledge of the occurrence, composition and function of peroxisomal contact sites. Studies in yeast strongly contributed...

...Ana Cláudia Leite; Telma S. Martins; Rute R. Cesário; Vitor Teixeira; Vítor Costa; Clara Pereira ABSTRACT Cdc28, the homolog of mammalian Cdk1, is a conserved key regulatory kinase for all major cell cycle transitions in yeast. We have found that defects in mitochondrial respiration (including...

... this approach, steep longitudinal gradients in CW expansion were measured in root hairs of Medicago truncatula ( Shaw et al., 2000 ; Dumais et al., 2004 ), with highest expansion rates at the apex ( Fig. 1 C). Similar results were also obtained in yeast and fungal hyphae ( Abenza et al., 2015 ; Bartnicki...

... to nutrients such as amino acids and glucose; however, the mechanisms underlying the activation of TORC2 signaling are still poorly understood compared to those for TORC1 signaling. In the budding yeast Saccharomyces cerevisiae , TORC2 targets the protein kinases Ypk1 and Ypk2 (hereafter Ypk1/2), and Pkc1...

..., owing in large part to the challenges associated with accurate determination of cell volume. Much of our comprehension of size regulation derives from yeast models, but even for these morphologically stereotypical cells, assessment of cell volume has mostly relied on proxies and extrapolations from two...

... ( Fanchiotti et al., 1998 ). By fluorescence microscopy, we observed that both Sp -GFP and Sp -GlyGFP indeed localized to a perinuclear ring and cortical sub-membrane region – the typical localization of ER proteins in fission yeast ( Fig. 3 C). To determine whether the biosensor could report...

...Marius Musielak; Carolin C. Sterk; Felix Schubert; Christian Meyer; Achim Paululat; Jürgen J. Heinisch ABSTRACT Rho5 is the yeast homolog of the human small GTPase Rac1. We characterized the genes encoding Rho5 and the subunits of its dimeric activating guanine-nucleotide-exchange factor (GEF...

.... Summary: Identification of cellular systems that impinge on protein homeostasis during arsenite stress in yeast, showing that transcriptional and translational control is central for mitigating protein aggregation and toxicity. Arsenic Protein misfolding Protein aggregation Transcription...

... in the plasma membrane of the living cells. We show here that a stable micron-scale protein-depleted region is generated in the plasma membrane of yeast mutants lacking phosphatidylserine at high temperatures. We named this region the ‘void zone’. Transmembrane proteins and certain peripheral membrane proteins...

..., the cell is not completely degraded through autophagy, implying the existence of some kind of termination mechanism. In the yeast Saccharomyces cerevisiae , autophagy is terminated after 10–12 h of nitrogen starvation. In this study, we found that termination is mediated by re-phosphorylation of Atg13...

... find that in Saccharomyces cerevisiae budding yeast, Cdc48 accumulates during cellular stress at intranuclear protein quality control sites (INQ). We show that Cdc48 function is required to suppress INQ formation under non-stress conditions and to promote recovery following genotoxic stress. Cdc48...

... to endocytic cargo. To test this idea, we analyzed the yeast adaptor protein Sla1, which binds membrane proteins harboring the endocytic signal NPFxD via the Sla1 SHD1 domain. Consistently, SHD1 domain point mutations that disrupted NPFxD binding caused a proportional reduction in Sla1–GFP recruitment...

...Fei Wu; Rinse de Boer; Arjen M. Krikken; Arman Akşit; Nicola Bordin; Damien P. Devos; Ida J. van der Klei ABSTRACT The yeast Hansenula polymorpha contains four members of the Pex23 family of peroxins, which characteristically contain a DysF domain. Here we show that all four H. polymorpha Pex23...

...Julian Chollet; Alexander Dünkler; Anne Bäuerle; Laura Vivero-Pol; Medhanie A. Mulaw; Thomas Gronemeyer; Nils Johnsson ABSTRACT Yeast cells select the position of their new bud at the beginning of each cell cycle. The recruitment of septins to this prospective bud site is one of the critical events...

... or mitochondrial matrix, which have nearly identical heme trafficking dynamics, potentially supporting a role for heme as a mitochondrial–nuclear retrograde signal. Moreover, we discovered that the heme synthetic enzyme 5-aminolevulinic acid synthase (ALAS, also known as Hem1 in yeast), and GTPases in control...

.... Nevertheless, when ER stress is prolonged or severe, cell death may occur, accompanied by production of mitochondrial reactive oxygen species (ROS). Using a yeast model ( Saccharomyces cerevisiae ), we describe an innate, adaptive response to ER stress to increase select mitochondrial proteins, O 2 consumption...

... that the yeast ( Saccharomyces cerevisiae ) nucleoporin Nup170, in addition to binding and silencing subtelomeric genes, supports transcription of genes regulated by the SAGA transcriptional activator complex. Specifically, we show that a lower amount of SAGA complex is bound to target genes in the absence...

...Lauren Budenholzer; Carolyn Breckel; Christopher M. Hickey; Mark Hochstrasser ABSTRACT The proteasome is an essential regulator of protein homeostasis. In yeast and many mammalian cells, proteasomes strongly concentrate in the nucleus. Sts1 from the yeast Saccharomyces cerevisiae is an essential...

...Oliver Glomb; Yehui Wu; Lucia Rieger; Diana Rüthnick; Medhanie A. Mulaw; Nils Johnsson ABSTRACT Owing to the local enrichment of factors that influence its dynamics and organization, the actin cytoskeleton displays different shapes and functions within the same cell. In yeast cells, post-Golgi...