... ) Competing interests The authors declare no competing or financial interests. 18 2 2016 2 5 2016 © 2016. Published by The Company of Biologists Ltd 2016 Summary: The chloride channel Ano1 regulates cell surface area in Xenopus oocytes by modulating the length of cellular...

... and Diffley, 2005 ; Stoeber et al., 1998 ; Whitmire et al., 2002 ). In Xenopus oocytes, Cdc6 is translated from maternal mRNAs and starts to accumulate 45–60 min after germinal vesicle breakdown (GVBD, which marks entry into the first meiotic division) ( Lemaître et al., 2002 ; Whitmire et al., 2002...

... ; [email protected] ) 22 10 2007 © The Company of Biologists Limited 2008 2008 Nuclear lamina Lamin filaments Xenopus oocyte feSEM Lamin A B-type lamins The nuclear lamina is a protein meshwork located between the inner nuclear membrane and the peripheral chromatin...

... by phosphorylation. Despite numerous studies showing that okadaic acid-sensitive phosphatases regulate both Cdc2 and Aurora-A activation, their identity has not yet been established in Xenopus oocytes and the importance of their regulation has not been evaluated. Using an oocyte cell-free system, we demonstrate...

... through inhibition of cyclin B-Cdc2 kinase activation. However, little is known about how the effector kinases are regulated when the checkpoint is attenuated. Recent studies indicate that Chk1 is also involved in the physiological G 2 -phase arrest of immature Xenopus oocytes via direct phosphorylation...

... produced conflicting results, we have examined the amplified nucleoli of Xenopus oocytes. These nucleoli are unique in that they contain high copy numbers of rRNA genes, are not attached to chromosomes, lack non-ribosomal DNA and can be examined in light microscopic spread preparations of nuclear contents...

...Anthi Karaïskou; Catherine Jessus; Thierry Brassac; René Ozon ABSTRACT The auto-catalytic activation of the cyclin-dependent kinase Cdc2 or MPF (M-phase promoting factor) is an irreversible process responsible for the entry into M phase. In Xenopus oocyte, a positive feed-back loop between Cdc2...

... Xenopus insulin receptor substrate-1 and blocks insulin-induced MAP kinase activation and germinal vesicle breakdown in Xenopus oocytes. In the current study we further examined the role of xROKα in insulin signal transduction in Xenopus oocytes. We demonstrate that injection of mRNA encoding the xROKα...

... cells but not in myoblasts, and of fibrillar structures in nuclei of Xenopus oocytes. In contrast, after methanol treatment, a 2G2-specific staining of stress fibres and myofibrils was observed, but no nuclear dot staining. We conclude that 2G2, in addition to binding to SDS- and methanol-denatured...

... study, we propose an alternative system allowing functional insertion of exogenous proteins into the plasma membrane of Xenopus oocytes. We microinjected proteoliposome sus-pensions into the cytoplasm and then analyzed membrane protein function. The proteins used in this work were members of the MIP...

... changes in chromosomal morphology and H1 kinase activity during a meiotic period from metaphase I (MI) to metaphase II (MII) in Xenopus oocytes. Using populations of oocytes that underwent germinal vesicle breakdown (GVBD) within a 10 minute interval, we found that the kinase activity declined gradually...

... analysis. In intact oocytes toxin B caused neither ADP-ribosylation nor phosphorylation of Rho. The data indicate that C. difficile toxin B acts on Rho proteins in Xenopus oocytes to inhibit ADP-ribosylation by C3. It is suggested that toxin B mediates its cytotoxic effect via functional inactivation...

... and discriminate between alternative partners, we have created chimeras composed of selected regions of rat connexin43, which forms channels with Xenopus connexin38, and rat connexin32, which cannot. Pairs of Xenopus oocytes were used to test the ability of the chimeras to form homotypic channels with themselves...