...Brittany J. Carr; Dominic Skitsko; Linnea M. Kriese; Jun Song; Zixuan Li; Myeong Jin Ju; Orson L. Moritz ABSTRACT Prominin-1 ( PROM1 ) variants are associated with inherited, non-syndromic vision loss. We used CRISPR/Cas9 to induce prom1 -null mutations in Xenopus laevis and then tracked retinal...

... cytoskeletal components are largely unknown. Here, we report that the septin cytoskeleton cooperates with actomyosin and microtubules to coordinate circumferential contraction of the wound margin and concentric elongation of wound-proximal cells in Xenopus laevis embryos. Microtubules reoriented radially...

... unexplored. Here, we examine the role of tight junctions during cytokinesis in the epithelium of the Xenopus laevis embryo. Depletion of the tight junction-associated proteins ZO-1 and GEF-H1 leads to altered cytokinesis duration and contractile ring geometry. Using a tension biosensor, we show...

..., Centralspindlin accumulates at overlapping central spindle microtubules and directs contractile ring formation by recruiting the GEF Ect2 to the cell equator to activate RhoA. We found that MgcRacGAP localized to the plus ends of equatorial astral microtubules during cytokinesis in Xenopus laevis embryos. How...

...-regulatory elements Intron Neurofilament Xenopus laevis Division of Integrative Organismal Systems 10.13039/100000154 1257449 American Association of University Women 10.13039/100005280 International Fellow Axon outgrowth during nervous system development requires...

... ventrally to contribute to most of the facial structures including bones, cartilage, muscles and ganglia. ADAM13 is a cell surface metalloprotease that is essential for CNC cell migration. Here, we show in Xenopus laevis embryos that the Wnt receptor Fz4 binds to the cysteine-rich domain of ADAM13...

... mediators of cell adhesion. However, a direct link between cadherin expression, differential adhesion and morphogenesis has not yet been determined for a specific process in vivo. To identify such a connection, we modulated the expression of C-cadherin in the Xenopus laevis gastrula, and combined...

... for correspondence (e-mail: [email protected] ) 24 8 2004 © The Company of Biologists Limited 2004 2004 Myosin Photoreceptors Actin Xenopus laevis Calycal process Class III unconventional myosins are actin filament-dependent motor proteins with N-terminal kinase domains...

...Stéphanie Lebreton; Laurent Boissel; Jacques Moreau Ras proteins mediate biological responses through various effectors and play a key role in relaying the Fibroblast Growth Factor (FGF) mesoderm induction signal during embryogenesis of the frog, Xenopus laevis . One Ras effector pathway involves...

... and differentiate into the secondary (adult) epithelium that resembles the mammalian counterpart. In the present study, to clarify whether Musashi-1(Msi-1), an RNA-binding protein, serves as a marker for progenitor cells of the adult epithelium, we chronologically examined Msi-1 expression in the Xenopus laevis...

... of the amphibian Xenopus laevis . The biosynthetic activity of these cells for production of their major cargo protein proopiomelanocortin (POMC) can be physiologically manipulated via the process of background adaptation (∼30-fold induction, with highly active cells in black toads and virtually inactive cells...

...Vernadeth B. Alarcón; Richard P. Elinson ABSTRACT The body plan of the embryo is established by a polarized source of developmental information in the oocyte. The Xenopus laevis oocyte creates polarity by anchoring mRNAs in the vegetal cortex, including Vg1 and Xwnt-11 , which might function...

... embryogenesis in Xenopus laevis . XCS-1 is a maternally expressed gene product that is the Xenopus homologue of the human cleavage signal protein (CS-1). XCS-1 transcripts were detected in oocytes with the titer decreasing just prior to the MBT. During development the XCS-1 protein was detected on the membrane...

... binding to all cargoes. * Author for correspondence (e-mail: [email protected] ) 14 10 1999 30 11 1999 © 1999 by Company of Biologists 1999 Brefeldin A Xenopus laevis Microtubule motor In most non-polarised animal cells the Golgi apparatus is a single copy...

.... * Authors for correspondence (e-mail: [email protected] ) † Present address: ICRF Laboratories, 44 Lincoln’s Inn Fields, London WC2A 3PX, UK 14 10 1997 © 1997 by Company of Biologists 1997 Ran RCC1 RanBP1 Nuclear structure Xenopus laevis Ran is an abundant...

... Centrin Centriole Centrosome Cell division cycle Ca 2+ -binding protein Xenopus laevis Fig. 1. Biochemical characterization of centrin in human cells. (A) Western blot analysis of low speed Triton X-100-soluble (S) and insoluble (I) fractions and of a fraction highly enriched in isolated...

... of Biologists 1995 pituitary secretory pathway differential screening Xenopus laevis During the last decade, considerable contributions have been made towards an understanding of the mechanisms by which proteins are secreted from eukaryotic cells. A number of the key molecules involved have...

... Biochemistry and Biophysics Program, Sloan Kettering Institute, New York, USA 28 04 1994 27 07 1994 © 1994 by Company of Biologists 1994 endosome acidification confocal microscopy pH determination yolk degradation vitellogenin Xenopus laevis In most non-mammalian...

...Michele Morse-Gaudio; Michael S. Risley ABSTRACT The relative content of topoisomerase II (topo II) and the induction of topo-II-mediated DNA damage and cellular abnormalities have been characterized in developing sper-matogenic cells of Xenopus laevis to gain an insight into the roles of topo II...

...Thomas J. Keating; R. John Cork; Kenneth R. Robinson ABSTRACT We have measured levels of intracellular free calcium ([Ca 2+ ] i ) in albino Xenopus laevis embryos using recombinant aequorin and a photon-counting system. We observed sinusoidal oscillations in [Ca 2+ ] i that had the same frequency...