... a cell-free system based on Xenopus egg extract, which allows the experimental separation of nuclear-membrane assembly and NPC formation. Nuclei surrounded by a closed double nuclear membrane, but devoid of NPCs, were first reconstituted from chromatin and a specific membrane fraction. Insertion of NPCs...

...Takayuki Kobayashi; Shusuke Tada; Takashi Tsuyama; Hiromu Murofushi; Masayuki Seki; Takemi Enomoto The response to DNA damage was analyzed using a cell-free system consisting of Xenopus egg extract and demembranated sperm nuclei. In the absence of DNA-damaging agents, detergent-resistant...

... Mitosis Xenopus egg extract In vitro spindle assembly Microtubule dynamics Microtubule-associated protein Acetylated tubulin Immunodepletion The accurate assembly of the mitotic spindle is essential for the segregation of sister chromatids during cell division, and requires complex...

...David Lourim; Georg Krohne ABSTRACT We have previously identified and quantitated three B-type lamin isoforms present in the nuclei of mature Xenopus laevis oocytes, and in cell-free egg extracts. As Xenopus egg extracts are frequently used to analyze nuclear envelope assembly and lamina functions...

... between nucleo-cytosolic ratio and origin specification when Chinese hamster ovary (CHO) cell nuclei are introduced into Xenopus egg extracts. At concentrations of intact late-G1-phase nuclei that approximate early Xenopus embryos, the entire genome was duplicated nearly 4 times faster than in culture...

...Zhi Hong Lu; Donald B. Sittman; David T. Brown; Rajan Munshi; Gregory H. Leno ABSTRACT We investigated the effects of histone H1s on DNA replication using Xenopus egg extract. Mouse variants H1c and H1 0 were assembled onto Xenopus sperm chromatin by the extract during the remodeling...

... not support those centers directly. * Author for correspondence 12 02 1997 28 06 1997 © 1997 by Company of Biologists 1997 Nuclear lamina Xenopus egg extract DNA replication Nuclear matrix The nuclear lamina is a filamentous meshwork composed of type V intermediate...

...Peter Bell; Ulrich Scheer ABSTRACT Nuclei assembled in Xenopus egg extract contain numerous spherical aggregations or nuclear bodies. Previously we have shown that they closely resemble prenucleolar bodies (PNBs), both at the compositional and ultrastructural level. Subsequently, coilin was also...

...Chuanmao Zhang; Hazel Jenkins; Martin W. Goldberg; Terry D. Allen; Christopher J. Hutchison ABSTRACT Nuclear lamina and matrices were prepared from sperm pronuclei assembled in Xenopus egg extracts using a fractionation and extraction procedure. Indirect immunofluorescence revealed that while...

... by Company of Biologists 1996 Xenopus egg extract Quantitation of oocyte lamin Lamin-associated egg vesicle Lamin synthesis When designing the comparative oocyte and egg protein synthesis experiments, our first concern was that both cell types come from the same frog. To accomplish...

... School of Medicine, Baltimore, MD, USA 20 12 1994 06 03 1995 © 1995 by Company of Biologists 1995 Xenopus egg extract in vitro nuclear reactivation quiescent cell nuclei erythrocyte red blood cell DNA synthesis in isolated nuclei heparin treatment of nuclei...

... 47 , 577 – 587 . 10.1016/0092-8674(86)90622-7 Blow , J. J. and Nurse P. ( 1990 ). A cdc2-like protein is involved in the initiation of DNA replication in Xenopus egg extracts . Cell 62 , 855 – 862 . 10.1016/0092-8674(90)90261-C Blow , J. J. and Sleeman...

... . 10.1084/jem.154.6.1899 Verheuen , R. , Van Venrooij , W. and Ramaekers , F. ( 1988 ). The nuclear matrix: structure and composition. J . Cell Sci . 90 , 11 – 36 . 10.1242/jcs.90.1.11 DNA replication replicon clusters Xenopus egg extract nuclear assembly confocal...