... investigated how topical insulin application would affect the diabetic wound healing process. We found that the excessive expression of insulin-degrading enzyme led to insufficient insulin levels in diabetic skin during wound healing, which ultimately reduced the recovery rate of diabetic wounds. We confirmed...

... factor activation Tissue repair Wound healing Myofibroblasts were first described in healing rat wound granulation tissue by Gabbiani and co-workers as cells that share morphological features of “ conventional tissue fibroblasts ” and contractile smooth muscle cells by being “ capable...

...Kristin J. Lastwika; Clarence A. Dunn; Joell L. Solan; Paul D. Lampe ABSTRACT The gap junction protein connexin 43 (Cx43) is a key player in wound healing, and inhibitors of Cx43, which speed epidermal wound healing, are currently in clinical trials. Here, we provide direct in vivo evidence...

.... Grainy head Epithelia Wound healing E-Cadherin Cytoskeleton Epithelia are the first line of defense against the surrounding environment in multicellular organisms. As barrier maintenance is critical for survival, epithelia need to be able to self-repair. Multiple molecular mechanisms are used...

...-fibrotic TGF-β1 and thus supports activation of myofibroblasts, which are main drivers of fibrosis. Myofibroblast Fibrosis Wound healing Transforming growth factor β1 TGF-β1 Growth factor activation Tissue fibrosis manifests as severe deformities in the skin and leads to reduced function...

... healing. Myofibroblast Integrin Fibrosis Scarring Wound healing Tissue scarring and fibrotic disease result from the activities and persistence of myofibroblasts that have failed to undergo apoptosis ( Abe et al., 2012 ; Hinz, 2007 ; Wilson et al., 2007 ). Myofibroblasts derive from...

... attributed. Summary: This study provides a newly identified mechanism by which H 2 O 2 -dependent oxidation of the inhibitor of κB kinase α and de-repression of epidermal growth factor promoter activity stimulates keratinocyte migration. Wound repair Wound healing Keratinocytes Migration...

... Summary: The focal adhesion protein Hic-5 plays a crucial role in myofibroblast differentiation in response to TGF-β by facilitating tension-dependent stress fiber assembly and MRTF-A nuclear accumulation. Hic-5 MRTF-A Mechanotransduction Myofibroblast Fibrosis Wound healing In normal...

... and HSP90AB1, respectively) work together to promote cell motility in wounded skin and accelerate wound closure. We demonstrate that Hsp90α and Hsp90β have distinct and non‐exchangeable functions during wound healing. Under hypoxia and when there is a lack of serum factors, Hsp90β binds to the cytoplasmic tail...

...Hiroya Takada; Kishio Furuya; Masahiro Sokabe ABSTRACT Cutaneous wound healing is accelerated by exogenous mechanical forces and is impaired in TRPC6-knockout mice. Therefore, we designed experiments to determine how mechanical force and TRPC6 channels contribute to wound healing using HaCaT...

... as the percentage of migration relative to control cells. For scratch wound healing assays, NIH-3T3, HeLa, Arl13b hnn or wild-type MEFs were grown to confluence and the monolayer scratched using 200 µl pipette plastic tips. After washing once with PBS, DMEM supplemented with 0.5% BSA was added together with PDGF...

...Vasilios Tsarouhas; Liqun Yao; Christos Samakovlis ABSTRACT Metazoans have evolved efficient mechanisms for epidermal repair and survival following injury. Several cellular responses and key signaling molecules that are involved in wound healing have been identified in Drosophila...

...Jingjing Li; Siwei Zhang; Ximena Soto; Sarah Woolner; Enrique Amaya Summary Embryonic wound healing provides a perfect example of efficient recovery of tissue integrity and homeostasis, which is vital for survival. Tissue movement in embryonic wound healing requires two functionally distinct actin...

... characterised, including the extracellular-signal-regulated kinases (ERKs) 1 and 2. In a small interfering RNA (siRNA)-based high-throughput wound healing screen performed in A549 lung carcinoma cells, we identified ERK2 but not ERK1 as a strong mediator of HGF-induced motility. This finding was confirmed...

... to be important for co-ordinating cellular responses during wound healing ( Kretz et al., 2003 ) and it has been proposed that Cx30 may play a crucial role ( Coutinho et al., 2003 ) in those events. Mice with ablation of the gene ( Gjb6 ) that encodes Cx30 (Cx30 null mice) are long-lived, breed and show...

...Amanda R. Brock; Yan Wang; Susanne Berger; Renate Renkawitz-Pohl; Violet C. Han; Yujane Wu; Michael J. Galko Summary Injury is an inevitable part of life, making wound healing essential for survival. In postembryonic skin, wound closure requires that epidermal cells recognize the presence of a gap...

... attracted the attention of researchers in other fields, as new functions of activin in angiogenesis, inflammation, immunity, fibrosis and cancer have been discovered. Studies from our laboratory have identified activin as a crucial regulator of wound healing and skin carcinogenesis. On the one hand...

.... * Authors for correspondence ( sukharev@umd.edu ; sachs@buffalo.edu ) © 2012. Published by The Company of Biologists Ltd 2012 Activin Cancer Tumor microenvironment Wound healing Cells and tissues respond to a variety of mechanical stresses that can be roughly classified as follows: (1...

... 7 2011 © 2012. 2012 α-Smooth muscle actin Contraction Matricellular protein Myofibroblast Periostin Wound healing Periostin is a secreted 90 kDa, disulfide-linked protein, which has structural similarity with the insect adhesion protein, fasciclin-1 ( Takeshita et al., 1993...

... factor in epidermal homeostasis, especially in epidermal aging and wound healing. Other functions might come to light after more in-depth investigations across different age groups using this model. Most interestingly, the type of inflammation noted might correspond to a new epidermal determinant...