..., a vital host defense mechanism. Multiciliated cell specification requires canonical Wnt signaling, which then must be turned off. Next, ciliogenesis and polarized ciliary orientation are regulated by noncanonical Wnt/planar cell polarity (Wnt/PCP) signaling. The mechanistic relationship between the Wnt...

...Konstantin Stoletov; Saray Sanchez; Irantzu Gorroño; Miriam Rabano; Maria d. M. Vivanco; Robert Kypta; John D. Lewis ABSTRACT Wnt signalling has been implicated as a driver of tumour cell metastasis, but less is known about which branches of Wnt signalling are involved and when they act...

... PtdIns P 2 Ror2 Wnt Kinase Phosphoinositide National Institutes of Health http://dx.doi.org/10.13039/100000002 R37-NS08174 R35-GM142690 National Science Foundation http://dx.doi.org/10.13039/100000001 IOS-1755004 This paper investigates in living cells...

... by osteoblasts to meet these synthetic demands are not well understood. WNT signaling is known to rapidly stimulate glutamine uptake during osteoblast differentiation. Using a cell biology approach, we identified two amino acid transporters, γ(+)-LAT1 and ASCT2 (encoded by Slc7 a7 and Slc1a5 , respectively...

... diffused freely in the extracellular space. This view has since changed following the discovery that morphogens of the Wnt and Hedgehog (Hh) families are modified by various lipid adducts during their biosynthesis, providing them with high affinity for the membrane bilayer. Recent work performed in model...

... has been shown to participate in signaling pathways, including Wnt signaling. XIAP regulates Wnt signaling by promoting the monoubiquitylation of the co-repressor Groucho/TLE family proteins, decreasing its affinity for the TCF/Lef family of transcription factors and allowing assembly...

...Jiaxin Sun; Shiyan Yu; Xiao Zhang; Catherine Capac; Onyedikachi Aligbe; Timothy Daudelin; Edward M. Bonder; Nan Gao ABSTRACT Wntless (Wls) transports Wnt molecules for secretion; however, the cellular mechanism underlying the initial assembly of Wnt secretory vesicles is still not fully defined...

... insight into the WNT-mediated regulation of the DNA damage response and suggest a novel role for the α-catenin–β-catenin complex in the nucleus. Here, we suggest that nuclear α-catenin influences the sensitivity of cells to DNA damage. We found that knockdown of α-catenin in a cell line that has...

... such oncogene, Musashi-1 ( Msi-1 ), regulates translation of target mRNAs and is implicated in promoting tumorigenesis in the colon and other tissues. Msi-1 targets include the tumor suppressor adenomatous polyposis coli ( Apc ), a Wnt pathway antagonist lost in ∼80% of all colorectal cancers. Cell culture...

...Austin T. Baldwin; Amy M. Clemons; Bryan T. Phillips ABSTRACT The Wnt/β-catenin signaling pathway is utilized across metazoans. However, the mechanism of signal transduction, especially dissociation of the β-catenin destruction complex by Dishevelled proteins, remains controversial. Here, we...

...Eliana Stanganello; Steffen Scholpp ABSTRACT Wnt signaling regulates a broad variety of processes during embryonic development and disease. A hallmark of the Wnt signaling pathway is the formation of concentration gradients by Wnt proteins across responsive tissues, which determines cell fate...

...Eric T. Hall; Esther M. Verheyen ABSTRACT Wnt/Wingless (Wg) and Ras–MAPK signaling both play fundamental roles in growth and cell fate determination, and when dysregulated, can lead to tumorigenesis. Several conflicting modes of interaction between Ras–MAPK and Wnt signaling have been identified...

...Bin Ma; Monika Fey; Michael O. Hottiger ABSTRACT The discovery of functional crosstalk between WNT and nuclear factor κB (NF-κB) signaling has established a more complex role for these two pathways in inflammation and cancer. However, the molecular mechanisms of the crosstalk and its biological...

... phosphorylation, thereby inhibiting EGF signaling in keratinocytes ( Xu et al., 2010 ). In addition, there is crosstalk between Wnt and TGF-β signaling; for example, it has been shown that TGF-β upregulates Tcf4 and increases Tcf4 transcriptional activity during osteoblast maturation ( McCarthy and Centrella...

...Simon W. Fellgett; Richard J. Maguire; Mary Elizabeth Pownall ABSTRACT Wnt signalling plays essential roles during embryonic development and is known to be mis-regulated in human disease. There are many molecular mechanisms that ensure tight regulation of Wnt activity. One such regulator...

... ventrally to contribute to most of the facial structures including bones, cartilage, muscles and ganglia. ADAM13 is a cell surface metalloprotease that is essential for CNC cell migration. Here, we show in Xenopus laevis embryos that the Wnt receptor Fz4 binds to the cysteine-rich domain of ADAM13...

...Dominic B. Bernkopf; Michel V. Hadjihannas; Jürgen Behrens ABSTRACT Axin and conductin (also known as axin2) are structurally related inhibitors of Wnt/β-catenin signalling that promote degradation of β-catenin. Whereas axin is constitutively expressed, conductin is a Wnt target gene implicated...

...Maria Gagliardi; Ana Hernandez; Ian J. McGough; Jean-Paul Vincent ABSTRACT A key step in the canonical Wnt signalling pathway is the inhibition of GSK3β, which results in the accumulation of nuclear β-catenin (also known as CTNNB1), and hence regulation of target genes. Evidence suggests...

...Nikolaos T. Georgopoulos; Lisa A. Kirkwood; Jennifer Southgate ABSTRACT By operating as both a subunit of the cadherin complex and a key component of Wnt signalling, β-catenin acts as the lynchpin between cell–cell contact and transcriptional regulation of proliferation, coordinating epithelial...

...Austin T. Baldwin; Bryan T. Phillips ABSTRACT The APC tumor suppressor regulates diverse stem cell processes including gene regulation through Wnt–β-catenin signaling and chromosome stability through microtubule interactions, but how the disparate functions of APC are controlled is not well...