...Francesca Patella; Daniel F. Cutler ABSTRACT The haemostatic protein von Willebrand factor (VWF) exists in plasma and subendothelial pools. The plasma pools are secreted from endothelial storage granules, Weibel–Palade bodies (WPBs), by basal secretion with a contribution from agonist-stimulated...

...Nicola L. Stevenson; Ian J. White; Jessica J. McCormack; Christopher Robinson; Daniel F. Cutler; Thomas D. Nightingale ABSTRACT Weibel-Palade bodies (WPBs), the storage organelles of endothelial cells, are essential to normal haemostatic and inflammatory responses. Their major constituent protein...

...Emma A. Cookson; Ianina L. Conte; John Dempster; Matthew J. Hannah; Tom Carter Summary Regulated secretion from endothelial cells is mediated by Weibel–Palade body (WPB) exocytosis. Plasma membrane cholesterol is implicated in regulating secretory granule exocytosis and fusion pore dynamics...

...Sofia Zografou; Dimitris Basagiannis; Alexandra Papafotika; Ryutaro Shirakawa; Hisanori Horiuchi; Daniel Auerbach; Mitsunori Fukuda; Savvas Christoforidis Summary Weibel–Palade bodies (WPBs) are endothelial-cell-specific organelles that, upon fusion with the plasma membrane, release cargo molecules...

..., 2002 ); however, little is known about protein mobilities within individual regulated secretory organelles (RSOs) ( Perez-Vilar et al., 2006 ). This is because most RSOs are small making direct analysis difficult. Endothelial cells contain large rod-like secretory granules, the Weibel-Palade bodies...

...Daniel J. Metcalf; Thomas D. Nightingale; Helen L. Zenner; Winnie W. Lui-Roberts; Daniel F. Cutler Weibel-Palade bodies (WPBs) are secretory organelles used for post-synthesis storage in endothelial cells that can, very rapidly, be triggered to release their contents. They carry a variety...

...Helen L. Zenner; Lucy M. Collinson; Grégoire Michaux; Daniel F. Cutler The Weibel-Palade bodies (WPBs) of endothelial cells play an important role in haemostasis and the initiation of inflammation, yet their biogenesis is poorly understood. Tubulation of their major content protein, von Willebrand...

... Fluorescence (TIRF) microscopy to visualize the three-dimensional motions of secretory organelles near the plasma membrane in living endothelial cells. Weibel-Palade bodies (WPb), the large tubular storage organelles for von Willebrand factor, were labelled with Rab27a-GFP. By contrast, green fluorescent...

... of proteins likely to be central to organelle identity is the Rab family of small GTPases. We have thus investigated Rab recruitment to membranes using endothelial cells as a model system. We report that Weibel-Palade bodies, the Von Willebrand Factor storage compartment of human umbilical vein endothelial...