... its C-terminal LD motif. Caskin2 also associates with the WAVE regulatory complex to promote cell migration in an Abi1-dependent manner. Furthermore, we demonstrate that the Caskin2–Abi1 interaction is regulated by growth factor-induced phosphorylation of Caskin2 on serine 878. In MCF7 and UACC893...

...Alexander Hirschhäuser; Marianne van Cann; Sven Bogdan ABSTRACT The WAVE regulatory complex (WRC) is the main activator of the Arp2/3 complex, promoting lamellipodial protrusions in migrating cells. The WRC is basally inactive but can be activated by Rac1 and phospholipids, and through...

...: This article provides insight into new functions of WASP family proteins from regulating the biogenesis of autophagosomes to recently identified roles in the nucleus. WASP N-WASP WAVE WHAMM WASH JMY WHAMY Arp2/3 Actin National Institutes of Health 10.13039/100000002 AI065474 AI20959...

... and actin polymerization is fundamental in cell migration, but a dynamic view of the underlying molecular mechanisms is still missing. The Rac1 GTPase controls actin polymerization at protrusions by interacting with its effector, the Wave regulatory complex (WRC). The exocyst complex, which functions...

.... Severe defects in myoblast fusion are caused by the loss of Kette (a homolog of Nap1 and Hem-2, also known as NCKAP1 and NCKAP1L, respectively), a member of the regulatory complex formed by Scar or WAVE proteins (represented by the single protein, Scar, in flies). kette mutants form finger-like...

...Ripla Arora; Emilie Abby; Adam D. J. Ross; Andrea V. Cantu; Michael D. Kissner; Vianca Castro; Hsin-Yi Henry Ho; Gabriel Livera; Diana J. Laird ABSTRACT Mouse ovarian germ cells enter meiosis in a wave that propagates from anterior to posterior, but little is known about contribution of germ cells...

... as WAVE), WASP and WASH (also known as CG13176), have been analyzed so far. Here, we characterized WHAMY, the fourth Drosophila WASP family member. whamy originated from a wasp gene duplication and underwent a sub-neofunctionalization. Unlike WASP, we found that WHAMY specifically interacted...

... ; Zalevsky et al., 2001 ). We therefore focus here on new data regarding two other Arp2/3-activating proteins, WAVE2 and HS1. Mammals express three WAVE isoforms ( Ibarra et al., 2005 ). WAVE1 and WAVE3 expression is strong in brain, whereas WAVE2 is widely expressed and the primary isoform in T cells...

..., the precise molecular mechanisms that underlie cell-cell adhesion formation remain unclear. Here we show that Wiskott-Aldrich syndrome protein family verprolin-homologous protein (WAVE)-mediated reorganization of actin, downstream of Rac plays an important role in normal development of cadherin-dependent cell...

.... and Kirschner, M. W. ( 2002 ). Mechanism of regulation of WAVE-1-induced actin nucleation by Rac1 and Nck. Nature 418 , 790 -793. Frischknecht, F. and Way, M. ( 2001 ). Surfing pathogens and the lessons learned for actin polymerization. Trends Cell Biol. 11 , 30 -38. Geese, M, Schluter, K...

... the driving force behind the formation and elongation. We tried to clarify how signals in response to extracellular cues are transformed to induce filopodial generation and extension. Observations on the formation process of filopodia at growth cones in the neuroblastoma cell line NG108 showed that WAVE (WASP...

... verprolin-homologous protein (WAVE) 2 than when activated by the VCA domain alone. By contrast, there was no difference between wild-type N-WASP or WAVE2 and VCA in the branching efficiency on de novo filaments, which are thought to consist mainly of ADP-phosphate filaments. This increased branching...

...Tadaomi Takenawa; Hiroaki Miki ABSTRACT Reorganization of cortical actin filaments plays critical roles in cell movement and pattern formation. Recently, the WASP and WAVE family proteins WASP and N-WASP, and WAVE1, WAVE2 and WAVE3 have been shown to regulate cortical actin filament reorganization...