... trafficking, we used fluorescent transferrin as a cargo for uptake experiments. We showed that silencing of either RhoU or ITSN2, but not ITSN1, increased transferrin accumulation in early endosomes, resulting from a defect in fast vesicle recycling. Concomitantly, RhoU and ITSN2 colocalized to a subset...

... of vesicles between cells in co-culture. These two Rab GTPases act in a cascade, where Rab11 activates Rab8 to form TNTs through VAMP3. Rab GTPase Tunneling nanotube Vesicle recycling Vesicle transfer Tunneling nanotubes (TNTs) are thin membranous structures that connect distant cells...