...Minjae Kim; Yixiang Zhong; Kyoung Hwa Jung; Young Gyu Chai; Bert Binas ABSTRACT Cultured rat primitive extraembryonic endoderm (pXEN) cells easily form free-floating multicellular vesicles de novo , exemplifying a poorly studied type of morphogenesis. Here, we reveal the underlying mechanism...

... vesicles and tubules involves extensive protein-mediated remodeling of the plasma membrane that is organized in space and time by protein–protein and protein–phospholipid interactions. Recent studies combining high-resolution imaging with genetic manipulations of the endocytic machinery...

... and the reserve pool of synaptic vesicles are affected. Additionally, axonal secretory organelles are abnormally distributed, whereas presynaptic proteins diminish at terminals and accumulate in distal axons, possibly in lysosomes. Our findings suggest that trafficking defects produced by Atlastin dysfunction...

... vesicle coat that mediates vesicular traffic from the endoplasmic reticulum. Sec31B is widely expressed and enriched in cerebellum and testis. Its predicted sequence of 1180 residues (expected molecular mass 128,711 Da) shares 47.3% and 18.8% similarity to human Sec31A (also known as Sec31; GenBank...

... signalling and localises to cytoplasmic puncta. It has been suggested that these Dvl-2-containing structures correspond to intracellular vesicles and may be involved in the Wnt signal transduction process. We report that cytoplasmic puncta are primarily formed in cells expressing Dvl-2 at high levels. Lower...

... microscopy suggests that synbindin is present on postsynaptic membranes and intracellular vesicles within dendrites, suggesting a role in postsynaptic membrane trafficking ( Iryna et al., 2000 ). Although a role of Trs23/synbindin remains to be examined in this particular context, its clear homology...

... vesiculation of elements of the Golgi complex would occur if transport vesicle formation continued under conditions where the consumption of these vesicles (i.e. their docking and fusion with target membranes) were blocked ( Misteli and Warren, 1994 ; Lowe et al., 1998a ). In apoptotic cells, incoming traffic...

...Greg Odorizzi; David J. Katzmann; Markus Babst; Anjon Audhya; Scott D. Emr Multivesicular bodies are late endosomal compartments containing lumenal vesicles that are formed by inward budding of the limiting endosomal membrane. In the yeast Saccharomyces cerevisiae , integral membrane proteins...

... that, in nucleated animal cells, the cytoplasm plays an active and essential role in resealing. A rapid and apparently chaotic membrane fusion response is initiated locally in the cytoplasm by the Ca 2+ that floods in through a disruption: cytoplasmic vesicles are thereby joined with one another(homotypically...

...Barbara A. Niemeyer; Thomas L. Schwarz ABSTRACT Fusion of vesicles with target membranes is dependent on the interaction of target (t) and vesicle (v) SNARE (soluble N SF (N-ethylmaleimide-sensitive fusion protein) a ttachment protein re ceptor) proteins located on opposing membranes. For fusion...

... to 3 hour period. We find that a substantial fraction of the integrins is left behind on the substratum as the cell detaches and locomotes, while another fraction collects into vesicles which are transported along the cell body as the cell migrates. As aggregates rip from the cell membrane...

... of extracellular vesicle-like structures on the endothelial sprouts. The vesicles formed on endothelial cell sprouts upon coculturing with tumor cells exhibit an increased amidolytic activity, suggestive of factor X/Xa activity, not dependent on tissue factor exposure. Experiments using HgCl 2 and Iodoacetamide...