...Sribalaji Lakshmikanthan; Magdalena Sobczak; Sergio Li Calzi; Lynn Shaw; Maria B. Grant; Magdalena Chrzanowska-Wodnicka ABSTRACT Vascular endothelial growth factor (VEGF), a key angiogenic and permeability factor, plays an important role in new blood vessel formation. However, abnormal VEGF-induced...

... internalises constitutively through clathrin-mediated endocytosis, whereas VEGF induces a new internalisation itinerary for VEGFR2 through macropinocytosis, which is essential for its signalling. Endocytosis Macropinocytosis Membrane trafficking Signalling VEGF VEGFR2 European Molecular...

... development of collaterals. Here, we report the role of AGS8 in vessel formation by endothelial cells. Knockdown of AGS8 by small interfering RNA (siRNA) inhibited vascular endothelial growth factor (VEGF)-induced tube formation, as well as VEGF-stimulated cell growth and migration. VEGF stimulated...

... A (VEGF-A). Dysregulation of these physiological processes contributes to the pathologies of heart disease, cancer and stroke. Rho GTPase proteins play an integral role in VEGF-mediated formation and maintenance of blood vessels. The regulatory functions of RhoA and RhoB in vasculogenesis and angiogenesis...

... of angiogenesis; however, its mechanism of action is not well understood. In the present study, we demonstrate that anastellin selectively inhibits microvessel cell signaling in response to the VEGF 165 isoform, but not VEGF 121 , by preventing the assembly of the complex containing the VEGF receptor...

... during normal development and in response to tumor angiogenic factors in vivo . Paxillin produces these effects by decreasing expression of neuropilin 2 (NRP2). Moreover, soluble factors secreted by tumors that stimulate vascular ingrowth, including vascular endothelial growth factor (VEGF), also...

... in vascular permeability induced by inflammatory stimuli and vascular endothelial growth factor (VEGF). Nitric oxide (NO) produced by endothelial NO synthase (eNOS) is crucial for VEGF-induced changes in permeability in vivo ; however, the molecular mechanism by which endogenous NO modulates endothelial...

...Wen Dong; Yi Li; Xiaoguang Li; Meiru Hu; Aodengqimuge; Li Sun; Ning Guo; Shengtao Yuan; Lun Song Summary Exposure to solar ultraviolet B (UVB) radiation is known to induce several pathological reactions in the skin. In these processes, upregulation of VEGF expression has been demonstrated...

.... The discovery of a circuit that integrates the inputs of circulatory flow and vascular endothelial growth factor (VEGF) signaling to modulate aortic arch angiogenesis, together with the expression of components of this circuit in the trunk vasculature, prompted us to investigate the role of these inputs...

...Mohammed A. Abdelsaid; Azza B. El-Remessy Summary Although promising, the ability to regulate angiogenesis through delivery of VEGF remains an unrealized goal. We have shown previously that physiological levels of peroxynitrite (1 µM) are required for a VEGF-mediated angiogenic response, yet...

...Nathaniel G. dela Paz; Tony E. Walshe; Lyndsay L. Leach; Magali Saint-Geniez; Patricia A. D'Amore Vascular endothelial growth factor (VEGF) plays a crucial role in developmental and pathological angiogenesis. Expression of VEGF in quiescent adult tissue suggests a potential role in the maintenance...

... of cardiovascular development and vascular integrity. We investigated vascular endothelial growth factor (VEGF)-mediated ERK5 activation in primary human dermal microvascular endothelial cells (HDMECs) undergoing proliferation on a gelatin matrix, and tubular morphogenesis within a collagen gel matrix. VEGF induced...

...Zhen Liu; Kazuki Kobayashi; Maarten van Dinther; Sandra H. van Heiningen; Gudrun Valdimarsdottir; Theo van Laar; Marion Scharpfenecker; Clemens W. G. M. Löwik; Marie-José Goumans; Peter ten Dijke; Evangelia Pardali Vascular endothelial growth factor (VEGF) and transforming growth factor-β (TGFβ...

...Elina Mattila; Kaisa Auvinen; Marko Salmi; Johanna Ivaska Vascular endothelial growth factor (VEGF) is a major angiogenic factor that triggers formation of new vessels under physiological and pathological conditions. However, the mechanisms that limit the VEGF responses in target cells and hence...

...Dawid G. Nowak; Jeanette Woolard; Elianna Mohamed Amin; Olga Konopatskaya; Moin A. Saleem; Amanda J. Churchill; Michael R. Ladomery; Steven J. Harper; David O. Bates Vascular endothelial growth factor A (VEGFA; hereafter referred to as VEGF) is a key regulator of physiological and pathological...

...Markus Ramsauer; Patricia A. D'Amore We used a 3D in-vitro model of angiogenesis to investigate the effects of different growth factors on vessel formation and stabilization in vitro. Vascular endothelial growth factor (VEGF) was the only factor that induced the formation, elongation and sprouting...

... aortic ECs (BAECs) and to block vascular endothelial growth factor (VEGF)-induced angiogenesis. Taken together, these results suggest that BMP-9 is a physiological ALK1 ligand that plays an important role in the regulation of angiogenesis. BAECs were seeded in 24-well plates and transiently...

...Mitsunobu R. Kano; Yasuyuki Morishita; Caname Iwata; Shigeru Iwasaka; Tetsuro Watabe; Yasuyoshi Ouchi; Kohei Miyazono; Keiji Miyazawa Combined stimulation with VEGF-A, FGF-2, or PDGF-BB has emerged as a potent strategy for therapeutic angiogenesis, although the mechanisms underlying the synergism...

...Yayoi Kaneko; Kenji Kitazato; Yuji Basaki Integrin-linked kinase (ILK) is one of the signaling moieties that interact with the cytoplasmic domains of integrin β1 and β3 subunits. Integrin-mediated outside-in signals cooperate with vascular endothelial growth factor (VEGF) receptor to promote...

... cell migration, vascular endothelial growth factor (VEGF) gradient-driven chemotaxis, capillary-like network formation and sprouting angiogenesis. In turn, soluble EphB4-Fc is pro-adhesive and stimulates EC migration and sprouting angiogenesis. EphrinB2-mediated repulsive signals are transduced...