..., we show that the application of force to endothelial cells stimulates VE-cadherin to activate liver kinase B1 (LKB1; also known as STK11) and AMP-activated protein kinase (AMPK), a master regulator of energy homeostasis. VE-cadherin-stimulated AMPK increases eNOS (also known as NOS3) activity...

... increases VE-cadherin phosphorylation and downregulation, accompanied by suppressed angiogenesis. Our findings not only highlight the role of intracellular signaling by TMEM16F in endothelial cells but also open new avenues for exploring the regulatory mechanisms for membrane lipid asymmetry...

..., AMPK agonists potently induced acute retinal vascular leakage. AMPK activation led to phosphorylation of endothelial nitric oxide synthase (eNOS, also known as NOS3), which in turn increased VE-cadherin (CDH5) phosphorylation on Y685. In parallel, AMPK also mediated phosphorylation of p38 MAP kinases...

... adherens junctions is a common feature of these diseases, but the role of BMPs in this process has not been investigated. BMP signaling is initiated by binding of ligand to, and activation of, BMP type I (BMPRI) and type II (BMPRII) receptors. Internalization of VE-cadherin as well as c-Src kinase...

...-GTPases is spatio-temporally controlled at endothelial adherens junctions by guanine-nucleotide exchange factors (GEFs) is incompletely understood. Here, we identify a crucial role for the Rho-GEF Trio in stabilizing junctions based around vascular endothelial (VE)-cadherin (also known as CDH5). Trio...

.... Moreover, knockdown of Solo significantly reduced cyclic‐stretch‐induced perpendicular reorientation of endothelial cells when cells were cultured at high density, but not when they were cultured at low density or pretreated with EGTA or VE‐cadherin‐targeting small interfering RNAs. These results suggest...

...Adrienne K. Barry; Ning Wang; Deborah E. Leckband ABSTRACT In this study, we present results demonstrating that mechanotransduction by vascular endothelial cadherin (VE-cadherin, also known as CDH5) complexes in endothelial cells triggers local cytoskeletal remodeling, and also activates global...

...) of leukocytes. The LBRC contains molecules involved in TEM, such as PECAM, PVR and CD99, but not VE-cadherin. Despite its importance, how membrane proteins are included in or excluded from the LBRC is not known. Immunoelectron microscopy and biochemical approaches demonstrate that inclusion into the LBRC...

... junctions and VE-cadherin (also known as cadherin 5), and reduced Rho activation and stress fiber formation. In addition, NO deficiency reduced VEGF-induced VE-cadherin phosphorylation and impaired the localization, but not the activation, of c-Src to cell junctions. The physiological role of eNOS...

...Armelle Le Guelte; Eva-Maria Galan-Moya; Julie Dwyer; Lucas Treps; Garance Kettler; Jagoda K. Hebda; Sonia Dubois; Cedric Auffray; Herve Chneiweiss; Nicolas Bidere; Julie Gavard Summary VE-cadherin-mediated cell–cell junction weakening increases paracellular permeability in response to both...

... (VE-) cadherin-mediated adhesion often precedes endothelial breakdown. This study aimed to stabilize VE-cadherin transinteraction and endothelial barrier function using peptides targeting the VE-cadherin adhesive interface. After modelling the transinteracting VE-cadherin structure, an inhibiting...

..., ischemia and diabetes – which leads to severe, and sometimes fatal, organ dysfunction. Endothelial permeability is regulated in part by the dynamic opening and closure of cell-cell adherens junctions (AJs). In endothelial cells, AJs are largely composed of vascular endothelial cadherin (VE-cadherin...

..., London, W6 8LH, UK § Present address: Faculty of Life Sciences, University of Manchester, Manchester, UK ¶ Present address: Ophthaltec Limited, London, SW1Y 4QU, UK 25 9 2007 © The Company of Biologists Limited 2008 2008 Lymphocyte migration VE-cadherin Tyrosine...

... this technique to determine a function for DP in the formation of microvascular tubes. * Author for correspondence (e-mail: [email protected] ) 30 1 2004 © The Company of Biologists Limited 2004 2004 Desmoplakin Cell adhesion Capillary VE-Cadherin Endothelial...

... application we demonstrated that the strength of adhesion of VE-cadherin-coated microspheres to the surface of cultured endothelial monolayers is significantly reduced by treatment with two well-established permeability-increasing compounds,cytochalasin D and the Ca 2+ -ionophore A23187, which shows that both...

...Sandra van Wetering; Jaap D. van Buul; Safira Quik; Frederik P. J. Mul; Eloise C. Anthony; Jean-Paul ten Klooster; John G. Collard; Peter L. Hordijk The integrity of the endothelium is dependent on cell-cell adhesion, which is mediated by vascular-endothelial (VE)-cadherin. Proper VE-cadherin...

...Peter L. Hordijk; Eloise Anthony; Frederik P. J. Mul; Ronald Rientsma; Lauran C. J. M. Oomen; Dirk Roos ABSTRACT Vascular endothelial (VE)-cadherin is the endothelium-specific member of the cadherin family of homotypic cell adhesion molecules. VE-cadherin, but not the cell adhesion molecule...