... of the TGN/EE and is maintained by the vacuolar H + -ATPase (V-ATPase) with support of proton-coupled antiporters. We show here that ClCd and ClCf, two distantly related members of the Arabidopsis Cl − channel (ClC) family, colocalize in the TGN/EE, where they act redundantly, and are essential for male...

... synthesis or Doa4 function causes a defect in sorting of a subset of ILV cargos. We show that these cargo-sorting defects are suppressed by mutations that disrupt Vph1, a subunit of vacuolar H + -ATPase (V-ATPase) complexes that acidify late endosomes and vacuoles. We further show that Vph1 dysfunction...

... previously unrecognized regulators of immune responses, while establishing close interactions with spermatozoa. RNA sequencing V-ATPase Sperm maturation Immune regulation Male reproductive tract Post-testicular regulation Male fertility Lalor Foundation Fellowship HD040793...

... interacts with the vacuolar (V)-ATPase subunit Vha55 that is crucial to establish and maintain lysosome acidification. As a consequence, starved flies that lack WASH function show a dramatic increase in acidic autolysosomes, causing a reduced lifespan. Thus, our data highlight a conserved role for WASH...

...Tianyi Zhang; Qingxiang Zhou; Margret Helga Ogmundsdottir; Katrin Möller; Robert Siddaway; Lionel Larue; Michael Hsing; Sek Won Kong; Colin Ronald Goding; Arnar Palsson; Eirikur Steingrimsson; Francesca Pignoni ABSTRACT The v-ATPase is a fundamental eukaryotic enzyme that is central to cellular...

...Ge-Hong Sun-Wada; Hiroyuki Tabata; Nobuyuki Kawamura; Minako Aoyama; Yoh Wada The nascent phagosome progressively establishes an acidic milieu by acquiring a proton pump, the vacuolar-type ATPase (V-ATPase). However, the origin of phagosomal V-ATPase remains poorly understood. We found...

...Ge-Hong Sun-Wada; Takao Toyomura; Yoshiko Murata; Akitsugu Yamamoto; Masamitsu Futai; Yoh Wada Vacuolar-type H + -ATPase (V-ATPase) is a multi-subunit enzyme that has important roles in the acidification of a variety of intracellular compartments and some extracellular milieus. Four isoforms...

...J. Du; L. Kean; A. K. Allan; T. D. Southall; S. A. Davies; C. J. McInerny; J. A. T. Dow V-ATPases play multiple roles in eukaryotes: in Drosophila , null mutations are recessive lethal. Here, mutations underlying five extant lethal alleles of vha55 , encoding the B subunit, were identified...

...Thomas Wassmer; Marine Froissard; Helmut Plattner; Roland Kissmehl; Jean Cohen The vacuolar proton-ATPase (V-ATPase) is a multisubunit enzyme complex that is able to transfer protons over membranes against an electrochemical potential under ATP hydrolysis. The enzyme consists of two subcomplexes: V...

... of trafficking. Moreover, we analyse the effect of the V-ATPase inhibitor bafilomycin A1 on endocytic uptake, which gives insight into the pH-dependent regulation of membrane trafficking and resorption in osteoclasts. References Altankov, G. and Grinnell, F. ( 1995 ). Fibronectin receptor internalization...

...Nicolas Morel; Jean-Claude Dedieu; Jean-Marc Philippe Vacuolar H + ATPase (V-ATPase) accumulates protons inside various intracellular organelles, generating the electrochemical proton gradient required for many vital cellular processes. V-ATPase is a complex enzyme with many subunits...

...Margaret Clarke; Jana Köhler; Quyen Arana; Tongyao Liu; John Heuser; Günther Gerisch The vacuolar H + -ATPase (V-ATPase) is a multi-subunit enzyme that plays important roles in eukaryotic cells. In Dictyostelium , it is found primarily in membranes of the contractile vacuole complex, where...

...Tongyao Liu; Christian Mirschberger; Lilian Chooback; Quyen Arana; Zeno Dal Sacco; Harry MacWilliams; Margaret Clarke The vacuolar proton pump (V-ATPase) appears to be essential for viability of Dictyostelium cells. To investigate the function of VatM, the 100 kDa transmembrane V-ATPase subunit, we...

...) Printed in Great Britain © The Company of Biologists Limited 1993 Is the gap junction channel - - made of connexin or ductin? Malcolm E. Finbow and John D. Pitts Key words: gap junction, connexon, connexin, ductin, V-ATPase 464 The protein component of connexin-free liver gap junc- tions and of arthropod...