.... In contrast to canonical macroautophagy, conjugation of ATG8s to single membranes (CASM) describes a group of non-canonical autophagy processes in which ATG8s are targeted to pre-existing single-membrane compartments. CASM occurs in response to disrupted intracellular pH gradients, when the V-ATPase proton...

...Ryan J. Mulligan; Magdalena M. Magaj; Laura Digilio; Stefanie Redemann; Chan Choo Yap; Bettina Winckler ABSTRACT Endosomal-lysosomal trafficking is accompanied by the acidification of endosomal compartments by the H + -V-ATPase to reach low lysosomal pH. Disruption of the correct pH impairs...

... , where its depletion results in tissue overgrowth and loss of cell polarity. Lgl links cell polarity and tissue growth through regulation of the Notch and the Hippo signalling pathways. Lgl regulates the Notch pathway by inhibiting V-ATPase activity via Vap33. How Lgl regulates the Hippo pathway...

... of the TGN/EE and is maintained by the vacuolar H + -ATPase (V-ATPase) with support of proton-coupled antiporters. We show here that ClCd and ClCf, two distantly related members of the Arabidopsis Cl − channel (ClC) family, colocalize in the TGN/EE, where they act redundantly, and are essential for male...

... synthesis or Doa4 function causes a defect in sorting of a subset of ILV cargos. We show that these cargo-sorting defects are suppressed by mutations that disrupt Vph1, a subunit of vacuolar H + -ATPase (V-ATPase) complexes that acidify late endosomes and vacuoles. We further show that Vph1 dysfunction...

... transgenic mice that express EYFP exclusively in mononuclear phagocytes (MPs) revealed intraepithelial projections of CD11c EYFP + MPs that are in close proximity to V-ATPase-positive narrow cells (the IS) and CCs (caput) ( Fig. 7 ; Movie 11 ). Moreover, IF confirmed that Cx3cl1 is highly expressed...

... interacts with the vacuolar (V)-ATPase subunit Vha55 that is crucial to establish and maintain lysosome acidification. As a consequence, starved flies that lack WASH function show a dramatic increase in acidic autolysosomes, causing a reduced lifespan. Thus, our data highlight a conserved role for WASH...

...Tianyi Zhang; Qingxiang Zhou; Margret Helga Ogmundsdottir; Katrin Möller; Robert Siddaway; Lionel Larue; Michael Hsing; Sek Won Kong; Colin Ronald Goding; Arnar Palsson; Eirikur Steingrimsson; Francesca Pignoni ABSTRACT The v-ATPase is a fundamental eukaryotic enzyme that is central to cellular...

...Ge-Hong Sun-Wada; Hiroyuki Tabata; Nobuyuki Kawamura; Minako Aoyama; Yoh Wada The nascent phagosome progressively establishes an acidic milieu by acquiring a proton pump, the vacuolar-type ATPase (V-ATPase). However, the origin of phagosomal V-ATPase remains poorly understood. We found...

...Ge-Hong Sun-Wada; Takao Toyomura; Yoshiko Murata; Akitsugu Yamamoto; Masamitsu Futai; Yoh Wada Vacuolar-type H + -ATPase (V-ATPase) is a multi-subunit enzyme that has important roles in the acidification of a variety of intracellular compartments and some extracellular milieus. Four isoforms...

...J. Du; L. Kean; A. K. Allan; T. D. Southall; S. A. Davies; C. J. McInerny; J. A. T. Dow V-ATPases play multiple roles in eukaryotes: in Drosophila , null mutations are recessive lethal. Here, mutations underlying five extant lethal alleles of vha55 , encoding the B subunit, were identified...

...Thomas Wassmer; Marine Froissard; Helmut Plattner; Roland Kissmehl; Jean Cohen The vacuolar proton-ATPase (V-ATPase) is a multisubunit enzyme complex that is able to transfer protons over membranes against an electrochemical potential under ATP hydrolysis. The enzyme consists of two subcomplexes: V...

... of trafficking. Moreover, we analyse the effect of the V-ATPase inhibitor bafilomycin A1 on endocytic uptake, which gives insight into the pH-dependent regulation of membrane trafficking and resorption in osteoclasts. * Author for correspondence (e-mail: [email protected] ) 9 10 2003 ©...

...Nicolas Morel; Jean-Claude Dedieu; Jean-Marc Philippe Vacuolar H + ATPase (V-ATPase) accumulates protons inside various intracellular organelles, generating the electrochemical proton gradient required for many vital cellular processes. V-ATPase is a complex enzyme with many subunits...

...Margaret Clarke; Jana Köhler; Quyen Arana; Tongyao Liu; John Heuser; Günther Gerisch The vacuolar H + -ATPase (V-ATPase) is a multi-subunit enzyme that plays important roles in eukaryotic cells. In Dictyostelium , it is found primarily in membranes of the contractile vacuole complex, where...

...Tongyao Liu; Christian Mirschberger; Lilian Chooback; Quyen Arana; Zeno Dal Sacco; Harry MacWilliams; Margaret Clarke The vacuolar proton pump (V-ATPase) appears to be essential for viability of Dictyostelium cells. To investigate the function of VatM, the 100 kDa transmembrane V-ATPase subunit, we...

... PI-3 kinase V-ATPase Osteoclasts are terminally differentiated multinucleated cells specialized for bone matrix degradation. These highly motile cells form a tight attachment to the mineralized bone matrix, the so-called sealing zone, upon an as yet unidentified signal. Formation...

...Tiina Laitala-Leinonen; Clemens Löwik; Socrates Papapoulos; H. Kalervo Väänänen ABSTRACT The role of proton transport and production in osteoclast differentiation was studied in vitro by inhibiting the transcription/translation of carbonic anhydrase II (CA II) and vacuolar H + -ATPase (V-ATPase...

... of Biologists 1996 V-ATPase Contractile vacuole Dictyostelium The vacuolar proton pump, or V-ATPase, is a multimeric enzyme found in all eukaryotic cells. The enzyme utilizes energy from the hydrolysis of ATP to drive protons across membranes. This proton gradient is employed to acidify...

... of the decorated tubules. In immunofluorescence studies, both polyclonal antibodies against the subunits of the V-ATPase of Dictyostelium discoideum and against the 57 kDa B-subunit of the V-ATPase of chromaffin granules gave identical labeling patterns to that produced by mAb DS-1. On cryosections, all three...