... and transport activity. Major facilitator superfamily MFS Transporters Monocarboxylic acids Endocytosis Arrestin Cytosolic termini Protein sorting Protein turnover Substrate specificity Ubiquitylation Fundação para a Ciência e a Tecnologia http://dx.doi.org/10.13039/501100001871...

... known as DNM1L). The E3 ubiquitin ligase MITOL (also known as MARCHF5) is known to ubiquitylate both MFN2 and DRP1. Interaction with and subsequent ubiquitylation by MITOL is stronger in the presence of wild-type MFN2 than with R364W-MFN2. This differential interaction of MITOL with MFN2 in the presence...

... phosphorylation at Ser68 and inhibited apoptosis in A549 cells. Mechanistically, CypA could mediate the phosphorylation of Twist1 at Ser68 via p38 mitogen-activated protein kinase (also known as MAPK14), which inhibited its ubiquitylation-mediated degradation. In addition, CypA increased interaction between...

... implicated in the production of WNT proteins. Here, we investigated how the WNT secretory factor EVI (also known as WLS) is ubiquitylated, recognised by ERAD components and subsequently removed from the secretory pathway. We performed a focused immunoblot-based RNAi screen for factors that influence EVI/WLS...

... a gene encoding an uncharacterized α-arrestin-like protein, Aly3/SPCC584.15c. α-arrestins are thought to recruit a ubiquitin ligase to membrane-associated proteins. Consistently, Ght5 is ubiquitylated in TORC2-deficient cells, and this ubiquitylation is dependent on Aly3. TORC2 supposedly enables cell...

... host to viral mRNAs are poorly understood. Here, we uncover a role for the ribosome-associated quality control (RQC) factor ZNF598 during vaccinia virus mRNA translation. ZNF598 acts on collided ribosomes to ubiquitylate 40S subunit proteins uS10 (RPS20) and eS10 (RPS10), initiating RQC-dependent...

... is ubiquitylated by the anaphase promoting complex/cyclosome (APC/C) and its cofactor Cdh1 (also known as FZR1) and is subsequently degraded in G1 phase. Cdh1 binds to regions within the N terminus of borealin that act as a non-canonical degron. Aurora B has also been shown previously to be degraded by the APC/C...

...Marie Le Boulch; Audrey Brossard; Gaëlle Le Dez; Sébastien Léon; Gwenaël Rabut ABSTRACT Ubiquitylation is a reversible post-translational protein modification that regulates a multitude of cellular processes. Detection of ubiquitylated proteins is often challenging because of their low abundance...

... migration. For instance, HACE1, a member of the ‘other HECTs’ family, reduces cell migration through ubiquitylating Rac1, a GTPase involved in cell migration, angiogenesis and cell growth ( Castillo-Lluva et al., 2013 ) ( Table S4 ). SMURF1 also impacts cell migration through the ubiquitylation of tumor...

... of TRIM32 was dependent on ATG7 and blocked by knockout of the five autophagy receptors p62 (also known as SQSTM1), NBR1, NDP52 (also known as CALCOCO2), TAX1BP1 and OPTN, pointing towards degradation by selective autophagy. p62 directed TRIM32 to lysosomal degradation, while TRIM32 mono-ubiquitylated p62...

...Ryohei Sakai; Ryosuke Fukuda; Shin Unida; Misaki Aki; Yuji Ono; Akinori Endo; Satoshi Kusumi; Daisuke Koga; Toshiaki Fukushima; Masayuki Komada; Tsukasa Okiyoneda ABSTRACT Endocytic trafficking is regulated by ubiquitylation (also known as ubiquitination) of cargoes and endocytic machineries...

...-132 treatment had no effect on serum starvation-induced cell quiescence under our experimental conditions. To examine whether CEP97 is ubiquitylated in cultured cells, HEK293T cells were co-transfected with YFP-tagged CEP97 and HA-ubiquitin, and cultured in the presence or absence of MG-132...

... in these processes. Using ubiquitylation-type-specific antibodies and Cx43 lysine-to-arginine mutants we show that ∼8% of a GJ, localized in central plaque domains, is K63-polyubiquitylated on K264 and K303. Levels and localization of ubiquitylation correlated well with: (1) the short turnover rate of Cxs and GJs...

... of transcriptionally active β-catenin–TCF/Lef complexes. We now demonstrate that XIAP is phosphorylated by GSK3 at threonine 180, and that an alanine mutant (XIAP T180A ) exhibits decreased Wnt activity compared to wild-type XIAP in cultured human cells and in Xenopus embryos. Although XIAP T180A ubiquitylates TLE3...

.... Here, we report that NEDD4 directly binds to SQSTM1 via its HECT domain and polyubiquitylates SQSTM1. This ubiquitylation is through K63 conjugation and is not involved in proteasomal degradation. Mutational analysis indicates that NEDD4 interacts with and ubiquitylates the PB1 domain of SQSTM1...

... Ubiquitylation Proteasome Protein degradation Acetylation/deacetylation Acetyldegron Lipopolysaccharide Cellular proteins are dynamic and stringently monitored in responding to endogenous and exogenous pathophysiological inputs. The ubiquitin proteasome system is a machinery that degrades most...

... to their unique C-termini. RET51, but not RET9, interacts with, and is ubiquitylated by CBL, which is recruited through interactions with the GRB2 adaptor protein. RET51 internalization was not affected by CBL knockout but was delayed in GRB2-depleted cells. In contrast, RET9 ubiquitylation requires...

...Pawel Leznicki; Yogesh Kulathu ABSTRACT Deubiquitylating (or deubiquitinating) enzymes (DUBs) are proteases that reverse protein ubiquitylation and therefore modulate the outcome of this post-translational modification. DUBs regulate a variety of intracellular processes, including protein turnover...

... modification required for Vpu-induced BST2 downregulation, through recruitment of the E3 ligase complex SCF adaptors β-TrCP1 and β-TrCP2 (two isoforms encoded by BTRC and FBXW11 , respectively). Herein, we further investigate the role of the ubiquitylation machinery in the lysosomal sorting of BST2. Using...

... with negative curvature. Yet, the mechanism for the regulation is unknown. Here, we show that MIM forms a complex with CXCR4 by binding to E3 ubiquitin ligase AIP4 (also known as ITCH) in response to stromal cell-derived factor 1 (SDF-1; also known as CXCL12). Overexpression of MIM promoted CXCR4 ubiquitylation...