...Revathi Sampath; Katherine Vaeth; Valeryia Mikalayeva; Vytenis Arvydas Skeberdis; Rytis Prekeris; Ke-Jun Han The Rab40 subfamily are unique small monomeric GTPases that form CRL5-based ubiquitin E3 ligase complex and regulate ubiquitylation of specific target proteins. Recent studies have shown...

... availability and cellular stress. Damaged or superfluous peroxisomes are removed mainly by pexophagy, the selective autophagy of peroxisomes induced by ubiquitylation of peroxisomal membrane proteins or ubiquitin-independent processes. Dysregulated pexophagy impairs peroxisome homeostasis and has been linked...

... with one another. At least three different E3 ligases have been implicated in ubiquitylation of the CP110–CEP97 capping complex: EDD1–DYRK2–DDB1 VPRBP , Cullin-KCTD and HERC2. These might function in concert to promote ubiquitylation and degradation of CP110 and CEP97. In addition, each or all of these E3...

... that reversible, non-degradative ubiquitylation regulates the function of the actin polymerase VASP in developing neurons. However, the underlying mechanism of how ubiquitylation impacts VASP activity was unknown. Here, we show that mimicking multi-monoubiquitylation of VASP at K240 and K286 negatively regulates...

.... Lem2 SUMOylation Ubiquitylation Schizosaccharomyces pombe Centromeres Wellcome Trust http://dx.doi.org/10.13039/100010269 202771/Z/16/Z Medical Research Council G1000505 Leverhulme Trust http://dx.doi.org/10.13039/501100000275 RPG-2014-050...

... and transport activity. Major facilitator superfamily MFS Transporters Monocarboxylic acids Endocytosis Arrestin Cytosolic termini Protein sorting Protein turnover Substrate specificity Ubiquitylation Fundação para a Ciência e a Tecnologia http://dx.doi.org/10.13039/501100001871...

... known as DNM1L). The E3 ubiquitin ligase MITOL (also known as MARCHF5) is known to ubiquitylate both MFN2 and DRP1. Interaction with and subsequent ubiquitylation by MITOL is stronger in the presence of wild-type MFN2 than with R364W-MFN2. This differential interaction of MITOL with MFN2 in the presence...

... phosphorylation at Ser68 and inhibited apoptosis in A549 cells. Mechanistically, CypA could mediate the phosphorylation of Twist1 at Ser68 via p38 mitogen-activated protein kinase (also known as MAPK14), which inhibited its ubiquitylation-mediated degradation. In addition, CypA increased interaction between...

... implicated in the production of WNT proteins. Here, we investigated how the WNT secretory factor EVI (also known as WLS) is ubiquitylated, recognised by ERAD components and subsequently removed from the secretory pathway. We performed a focused immunoblot-based RNAi screen for factors that influence EVI/WLS...

... a gene encoding an uncharacterized α-arrestin-like protein, Aly3/SPCC584.15c. α-arrestins are thought to recruit a ubiquitin ligase to membrane-associated proteins. Consistently, Ght5 is ubiquitylated in TORC2-deficient cells, and this ubiquitylation is dependent on Aly3. TORC2 supposedly enables cell...

... host to viral mRNAs are poorly understood. Here, we uncover a role for the ribosome-associated quality control (RQC) factor ZNF598 during vaccinia virus mRNA translation. ZNF598 acts on collided ribosomes to ubiquitylate 40S subunit proteins uS10 (RPS20) and eS10 (RPS10), initiating RQC-dependent...

... is ubiquitylated by the anaphase promoting complex/cyclosome (APC/C) and its cofactor Cdh1 (also known as FZR1) and is subsequently degraded in G1 phase. Cdh1 binds to regions within the N terminus of borealin that act as a non-canonical degron. Aurora B has also been shown previously to be degraded by the APC/C...

...Marie Le Boulch; Audrey Brossard; Gaëlle Le Dez; Sébastien Léon; Gwenaël Rabut ABSTRACT Ubiquitylation is a reversible post-translational protein modification that regulates a multitude of cellular processes. Detection of ubiquitylated proteins is often challenging because of their low abundance...

... they contribute to disease development. Ubiquitin HECT E3 ubiquitin ligase Ubiquitylation Protein-protein interactions Protein turnover Cell signaling Cancer Neurological disorders Neurodevelopmental disorders Neurodegeneration National Institutes of Health 10.13039/100000002...

... of TRIM32 was dependent on ATG7 and blocked by knockout of the five autophagy receptors p62 (also known as SQSTM1), NBR1, NDP52 (also known as CALCOCO2), TAX1BP1 and OPTN, pointing towards degradation by selective autophagy. p62 directed TRIM32 to lysosomal degradation, while TRIM32 mono-ubiquitylated p62...

...Ryohei Sakai; Ryosuke Fukuda; Shin Unida; Misaki Aki; Yuji Ono; Akinori Endo; Satoshi Kusumi; Daisuke Koga; Toshiaki Fukushima; Masayuki Komada; Tsukasa Okiyoneda ABSTRACT Endocytic trafficking is regulated by ubiquitylation (also known as ubiquitination) of cargoes and endocytic machineries...

... CEP97 CP110 Cullin-3 KCTD10 Ubiquitylation Japan Society for the Promotion of Science 10.13039/501100001691 15H04347 15H01197 18H02398 16K20910 Primary cilia are non-motile cilia protruding from the plasma membrane of the majority of vertebrate cells. They function...

... in these processes. Using ubiquitylation-type-specific antibodies and Cx43 lysine-to-arginine mutants we show that ∼8% of a GJ, localized in central plaque domains, is K63-polyubiquitylated on K264 and K303. Levels and localization of ubiquitylation correlated well with: (1) the short turnover rate of Cxs and GJs...

... of transcriptionally active β-catenin–TCF/Lef complexes. We now demonstrate that XIAP is phosphorylated by GSK3 at threonine 180, and that an alanine mutant (XIAP T180A ) exhibits decreased Wnt activity compared to wild-type XIAP in cultured human cells and in Xenopus embryos. Although XIAP T180A ubiquitylates TLE3...

.... Here, we report that NEDD4 directly binds to SQSTM1 via its HECT domain and polyubiquitylates SQSTM1. This ubiquitylation is through K63 conjugation and is not involved in proteasomal degradation. Mutational analysis indicates that NEDD4 interacts with and ubiquitylates the PB1 domain of SQSTM1...