... to γ2-bearing COPI subcomplexes. Therefore, Scyl1 functions as a scaffold for key components of COPI coats, and disruption of the scaffolding function of Scyl1 causes tubulation of the endoplasmic reticulum (ER)-Golgi intermediate compartment (ERGIC) and the cis-Golgi, similar to that observed following...

... microtubule network is not required to maintain these tubules once formed. We make a distinction here between other data which has investigated the role of microtubules in formation of the SNX-coated tubules (e.g. Kerr et al., 2006 ) versus our experiments which really only address the role of microtubules...

..., are on contractile vacuoles. MEGAP1 – /2 – cells empty their contractile vacuoles less efficiently than normal and consequently have three times the usual number. GFP-tagged MEGAP1 localises to tubules of the contractile vacuole network and when vacuoles start to empty they recruit cytosolic GFP-MEGAP1. Mutants...