... involved in the regulation of microtubule dynamics and function in eukaryotes. In the protozoan parasite Trypanosoma brucei , the microtubule cytoskeleton is essential for cell motility and maintaining cell shape. In a previous study, we showed that T. brucei TTLL6A and TTLL12B are required to regulate...

... Riemer; Tuguldur Tumurbaatar; Kristina Djinovic-Carugo; Gang Dong; Christian J. Janzen; Brooke Morriswood ABSTRACT All endocytosis and exocytosis in the African trypanosome Trypanosoma brucei occurs at a single subdomain of the plasma membrane. This subdomain, the flagellar pocket, is a small vase-shaped...

...Miharisoa Rijatiana Ramanantsalama; Nicolas Landrein; Elina Casas; Bénédicte Salin; Corinne Blancard; Mélanie Bonhivers; Derrick R. Robinson; Denis Dacheux ABSTRACT In Trypanosoma brucei , transition fibres (TFs) form a nine-bladed pattern-like structure connecting the base of the flagellum...

...Simona Amodeo; Ana Kalichava; Albert Fradera-Sola; Eloïse Bertiaux-Lequoy; Paul Guichard; Falk Butter; Torsten Ochsenreiter ABSTRACT Proper mitochondrial genome inheritance is important for eukaryotic cell survival. Trypanosoma brucei , a protozoan parasite, contains a singular mitochondrial genome...

...Jana Jentzsch; Adal Sabri; Konstantin Speckner; Gertrud Lallinger-Kube; Matthias Weiss; Klaus Ersfeld ABSTRACT The shape of kinetoplastids, such as Trypanosoma brucei , is precisely defined during the stages of the life cycle and governed by a stable subpellicular microtubule cytoskeleton. During...

...Robert L. Douglas; Brett M. Haltiwanger; Anna Albisetti; Haiming Wu; Robert L. Jeng; Joel Mancuso; W. Zacheus Cande; Matthew D. Welch ABSTRACT Trypanosoma brucei , the causative agent of African sleeping sickness, has a flagellum that is crucial for motility, pathogenicity, and viability. In most...

...Tai An; Qing Zhou; Huiqing Hu; Harshini Cormaty; Ziyin Li ABSTRACT The human parasite Trypanosoma brucei transitions from the trypomastigote form to the epimastigote form in the insect vector by repositioning its mitochondrial genome and flagellum-associated cytoskeleton. The molecular mechanisms...

...Jonathan C. Miller; Stephanie B. Delzell; Jeniffer Concepción-Acevedo; Michael J. Boucher; Michele M. Klingbeil ABSTRACT Mitochondrial DNA of Trypanosoma brucei and related parasites is a catenated network containing thousands of minicircles and tens of maxicircles, called kinetoplast DNA (kDNA...

...Midori Ishii; Bungo Akiyoshi ABSTRACT The kinetochore is a macromolecular protein complex that drives chromosome segregation in eukaryotes. Unlike most eukaryotes that have canonical kinetochore proteins, evolutionarily divergent kinetoplastids, such as Trypanosoma brucei , have unconventional...

...Kieu T. M. Pham; Qing Zhou; Yasuhiro Kurasawa; Ziyin Li ABSTRACT Trypanosoma brucei possesses a motile flagellum that determines cell morphology and the cell division plane. Inheritance of the newly assembled flagellum during the cell cycle is controlled by the Polo-like kinase homolog TbPLK, which...

...-exchange factor (GEF) for Arl3 and is implicated in a variety of ciliary and cellular functions. We have identified and characterized Trypanosoma brucei (Tb)Arl13, the sole Arl13b homolog in this evolutionarily divergent, protozoan parasite . TbArl13 has conserved flagellar functions and exhibits catalytic...

... against cellular stresses and apoptosis, indicating the multifunctional role of the protein. Here, for the first time, we characterize the expression and function of TCTP in the human and animal pathogen, Trypanosoma brucei . We identified two paralogs ( TCTP1 and TCTP2 ) that are differentially expressed...

...Simona Amodeo; Martin Jakob; Torsten Ochsenreiter ABSTRACT The unicellular parasite Trypanosoma brucei harbors one mitochondrial organelle with a singular genome called the kinetoplast DNA (kDNA). The kDNA consists of a network of concatenated minicircles and a few maxicircles that form the kDNA...

... of cytokinesis. Cytokinesis CIF1 CIF2 Zinc finger EF hand Trypanosoma brucei National Institutes of Health 10.13039/100000002 AI101437 Austrian Science Fund 10.13039/501100002428 P28231-B28 Trypanosoma brucei , an early branching parasitic protozoan, causes...

...Amy N. Sinclair-Davis; Michael R. McAllaster; Christopher L. de Graffenried ABSTRACT The parasite Trypanosoma brucei is highly polarized, including a flagellum that is attached along the cell surface by the flagellum attachment zone (FAZ). During cell division, the new FAZ positions the cleavage...

... revealed a precisely defined 3D organisation for both the flagellar pocket and FAZ, with striking similarities to those of Trypanosoma brucei . Expression of two T. brucei FAZ proteins in L. mexicana showed that T. brucei FAZ proteins can assemble into the Leishmania FAZ structure. Leishmania therefore...

...Qing Zhou; Huiqing Hu; Cynthia Y. He; Ziyin Li ABSTRACT Adhesion of motile flagella to the cell body in Trypanosoma brucei requires a filamentous cytoskeletal structure termed the flagellum attachment zone (FAZ). Despite its essentiality, the complete molecular composition of the FAZ filament...

... that is highly enriched in, and present throughout, the plasma membrane – has been implicated in endocytosis. Trypanosoma brucei has one of the highest known rates of endocytosis, a process it uses to evade the immune system. To determine whether phosphoinositides play a role in endocytosis in this organism, we...

...Brice Rotureau; Thierry Blisnick; Ines Subota; Daria Julkowska; Nadège Cayet; Sylvie Perrot; Philippe Bastin ABSTRACT The Trypanosoma brucei flagellum is an essential organelle anchored along the surface of the cell body through a specialized structure called the flagellum attachment zone (FAZ...

...Louise Hughes; Katie Towers; Tobias Starborg; Keith Gull; Sue Vaughan Summary Flagella are highly conserved organelles present in a wide variety of species. In Trypanosoma brucei the single flagellum is necessary for morphogenesis, cell motility and pathogenesis, and is attached along the cell body...