... the light path is split to visualise multiple focal planes simultaneously, was used to reconstruct Trypanosoma brucei and Leishmania mexicana movement in 3D. These species are uniflagellate unicellular parasites for which motility is vital. It was possible to use either a fluorescent stain or a genetically...

... comprehensive trypanosome clathrin interactome to date. We identified the trypanosome AP-1 complex, Trypanosoma brucei (Tb)EpsinR, several endosomal SNAREs plus orthologues of SMAP and the AP-2 associated kinase AAK1 as interacting with clathrin. Novel lineage-specific proteins were identified, which we...

... Euglenozoa genomes. Bodonids possess a large trafficking repertoire, which is mainly retained by the Trypanosoma cruzi group, with extensive losses in other lineages, particularly African trypanosomes and phytomonads. There are no large-scale expansions or contractions from an inferred ancestor, excluding...

... a heterologous expression system in yeast. Whereas Trypanosoma brucei DOT1A predominantly dimethylated H3K79, DOT1B trimethylated H3K79 even in the absence of dimethylation by DOT1A. Furthermore, DOT1A activity was selectively reduced by eliminating ubiquitylation of H2B. The tail of histone H4 was not required...

.... Cell Dev. Biol. 14 , 111 -136. Brown, D. A. and London, E. ( 2000 ). Structure and function of sphingolipid- and cholesterol-rich membrane rafts. J. Biol. Chem. 275 , 17221 -17224. Coppens, I. and Courtoy, P. J. ( 2000 ). The adaptative mechanisms of Trypanosoma brucei for sterol...

...@northwestern.edu ) 17 11 2008 © The Company of Biologists Limited 2009 2009 Flagellum Palmitoylation Membrane microdomains Acylation Trypanosoma Protein palmitoylation is the thioester linkage of long chain fatty acids, most commonly palmitic acid, to cysteine residues of protein...

...; however, recent studies in trypanosomes point to the dispensability of sphingolipids in some of these processes. In this study, we explore the requirements for de novo sphingolipid biosynthesis in the insect life cycle stage of the African trypanosome Trypanosoma brucei by inhibiting the enzyme serine...

...Matthew J. Helms; Audrey Ambit; Paul Appleton; Laurence Tetley; Graham H. Coombs; Jeremy C. Mottram Trypanosoma brucei possesses five metacaspase genes. Of these, MCA2 and MCA3 are expressed only in the mammalian bloodstream form of the parasite, whereas MCA5 is expressed also in the insect...

...Helen P. Price; Chrysoula Panethymitaki; David Goulding; Deborah F. Smith Myristoyl-CoA:protein N -myristoyltransferase (NMT), an essential protein in Trypanosoma brucei and Leishmania major , catalyses the covalent attachment of the fatty acid myristate to the N-terminus of a range of target...

... of yeasts and mammals. The PEX2 gene of Trypanosoma brucei was identified and its expression was silenced by means of tetracycline-inducible RNA interference. Bloodstream-form trypanosomes, which rely exclusively on glycolysis for ATP generation, died rapidly upon PEX2 depletion. Insect-form (procyclic...

...Michael T. Dolan; Colin G. Reid; H. Paul Voorheis ABSTRACT Calcium ions (100μM) were found to initiate the selective and complete depolymerization of the pellicular microtubules of Trypanosoma brucei . The Ca 2+ -dependent release of tubulin was found to occur without the detectable mediation...