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1-20 of 21
Keywords: Translocation
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Journal Articles
Journal:
Journal of Cell Science
J Cell Sci (2014) 127 (14): 3017–3023.
Published: 15 July 2014
... of the secretome transiently resides in the cytosol before translocating into the ER, both in yeast and in higher eukaryotes. To uncover factors that monitor this transient cytosolic protein pool, we carried out a genetic screen in Saccharomyces cerevisiae . Our findings highlighted a pre-insertional degradation...
Includes: Supplementary data
Journal Articles
Journal:
Journal of Cell Science
J Cell Sci (2011) 124 (24): 4184–4193.
Published: 15 December 2011
...Hidenobu Fujita; Marifu Yamagishi; Yuichiro Kida; Masao Sakaguchi Polypeptide chains synthesized by membrane-bound ribosomes are translocated through, and integrated into, the endoplasmic reticulum (ER) membrane by means of the protein translocation channel, the translocon. Positive charges...
Includes: Supplementary data
Journal Articles
Journal:
Journal of Cell Science
J Cell Sci (2008) 121 (18): 3015–3024.
Published: 15 September 2008
... in the cerebellum. This enzyme requires two events for its full activation: Ca 2+ -dependent translocation from the cytosol to organelle membranes in order to access phospholipids as substrates, and phosphorylation by several kinases. However, the subcellular distribution and activation of cPLA 2 α in Purkinje...
Includes: Multimedia, Supplementary data
Journal Articles
Montserrat Rodríguez, Gerard Torrent, Montserrat Bosch, Fabienne Rayne, Jean-François Dubremetz, Marc Ribó, Antoni Benito, Maria Vilanova, Bruno Beaumelle
Journal:
Journal of Cell Science
J Cell Sci (2007) 120 (8): 1405–1411.
Published: 15 April 2007
... delivery. This intracellular destination is specific to Onconase because other (and much less toxic) RNases follow the default pathway to late endosomes/lysosomes. Drugs neutralizing endosomal pH increased Onconase translocation efficiency from purified endosomes during cell-free translocation assays...
Includes: Supplementary data
Journal Articles
Vigdis Sørensen, Antoni Wiedlocha, Ellen Margrethe Haugsten, Denis Khnykin, Jørgen Wesche, Sjur Olsnes
Journal:
Journal of Cell Science
J Cell Sci (2006) 119 (20): 4332–4341.
Published: 15 October 2006
... and FGF-2 are endocytosed and translocated to the cytosol and nucleus where they stimulate RNA and DNA synthesis. Here we have studied the ability of the four FGF receptors to facilitate translocation of exogenous FGF-1 to the cytosol and nucleus. FGFR1 and FGFR4 were able to mediate translocation...
Journal Articles
Journal:
Journal of Cell Science
J Cell Sci (2006) 119 (14): 2935–2944.
Published: 15 July 2006
..., the rhabdomere, in the dark, and undergo light-induced translocation into the cell body as a mechanism for long-term light-adaptation. We show that translocation of TRPL channels occurs in two distinct stages, first to the neighboring stalk membrane then to the basolateral membrane. In the first stage, light...
Journal Articles
Journal:
Journal of Cell Science
J Cell Sci (2006) 119 (13): 2826–2836.
Published: 1 July 2006
..., J. E., Dascher-Nadel, C. and Lindley, I. J. ( 2005 ). Selective inhibition of cotranslational translocation of vascular cell adhesion molecule 1. Nature 436 , 290 -293. Booth, P. J. and High, S. ( 2004 ). Polytopic membrane protein folding and assembly in vitro and in vivo. Mol. Membr...
Journal Articles
Journal:
Journal of Cell Science
J Cell Sci (2004) 117 (20): 4797–4806.
Published: 15 September 2004
...Michelle A. Cronin; Fengqiu Diao; Susan Tsunoda We examine the light-dependent subcellular translocation of the visual G q α protein between the signaling compartment, the rhabdomere and the cell body in Drosophila photoreceptors. We characterize the translocation of G q α and provide the first...
Journal Articles
Journal:
Journal of Cell Science
J Cell Sci (2004) 117 (18): 4089–4098.
Published: 15 August 2004
... © The Company of Biologists Limited 2004 2004 Cyclin-dependent kinase Src family kinase Translocation Wound healing Transgenic mice Cdk5 is a proline-directed protein kinase that is closely related to the cyclin-dependent kinases. Cdk5 and its activator, p35, are expressed...
Journal Articles
Journal:
Journal of Cell Science
J Cell Sci (2003) 116 (17): 3511–3518.
Published: 1 September 2003
... state. Insulin does not further enhance the cell-surface level of HA-GLUT4-Akt2-wt, but does stimulate the translocation of HA-GLUT4-Akt2-KD. Cell-surface HA-GLUT4-Akt2-wt is found to be phosphorylated on Ser 474 in both the absence and presence of insulin, and mutation of Ser 474 to Ala reduces...
Journal Articles
Journal:
Journal of Cell Science
J Cell Sci (2002) 115 (24): 4947–4956.
Published: 15 December 2002
...Anita Boisramé; Marion Chasles; Anna Babour; Jean-Marie Beckerich; Claude Gaillardin The core component of the translocation apparatus, Sec61p or α, was previously cloned in Yarrowia lipolytica . Using anti-Sec61p antibodies, we showed that most of the translocation sites are devoted to co...
Journal Articles
Journal:
Journal of Cell Science
J Cell Sci (2001) 114 (13): 2491–2499.
Published: 1 July 2001
...Fernando A. Agarraberes; J. Fred Dice A group of cytosolic proteins are targeted to lysosomes for degradation in response to serum withdrawal or prolonged starvation by a process termed chaperone-mediated autophagy. In this proteolytic pathway little is known about how proteins are translocated...
Journal Articles
Journal:
Journal of Cell Science
J Cell Sci (2000) 113 (8): 1335–1343.
Published: 15 April 2000
... a transient translocation of PKC ε, γ, and δ from the cytoplasm to the plasma membrane. These translocations were blocked by an inhibitor of phosphatidylinositol-specific phospholipase C (PLC), but not by an inhibitor of phosphatidylcholine- specific PLC. A diacylglycerol (DAG) analogue also induced...
Journal Articles
Journal:
Journal of Cell Science
J Cell Sci (1999) 112 (23): 4185–4191.
Published: 1 December 1999
... 1999 Yeast Translocation ER Sec61 Quality control Protein secretion is an essential process. In most cases, however, cells are better off secreting a reduced amount of a specific protein than secreting a defective form of this protein. Misfolded secreted and cell surface proteins...
Journal Articles
Journal:
Journal of Cell Science
J Cell Sci (1999) 112 (10): 1477–1486.
Published: 15 May 1999
...Julia J. A. Greenfield; Stephen High ABSTRACT The heteromeric Sec61 complex is composed of α, β and γ subunits and forms the core of the mammalian ER translocon. Oligomers of the Sec61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane...
Journal Articles
Jon Broughton, Dominique Swennen, Barrie M. Wilkinson, Philippe Joyet, Claude Gaillardin, Colin J. Stirling
Journal:
Journal of Cell Science
J Cell Sci (1997) 110 (21): 2715–2727.
Published: 1 November 1997
...Jon Broughton; Dominique Swennen; Barrie M. Wilkinson; Philippe Joyet; Claude Gaillardin; Colin J. Stirling ABSTRACT The Sec61 protein is required for protein translocation across the ER membrane in both yeast and mammals and is found in close association with polypeptides during their membrane...
Journal Articles
Journal:
Journal of Cell Science
J Cell Sci (1997) 110 (19): 2417–2427.
Published: 1 October 1997
... and N19RhoA, however, are mainly cytosolic. We find that LPA-induced shape changes are preceded by translocation of RhoA from the cytosol to the cell periphery. LPA also stimulates translocation of inactive N19RhoA in the absence of ensuing shape changes. When membrane localization of RhoA is prevented...
Journal Articles
Journal:
Journal of Cell Science
J Cell Sci (1996) 109 (11): 2601–2608.
Published: 1 November 1996
... together with the structures and putative functions of the novel SRP-related ribonucleoprotein particles. * Author for correspondence (e-mail: Strub@Sc2a.unige.ch ) © 1996 by Company of Biologists 1996 Translocation ER Alu RNPs 7SL RNA Translational elongation Fig. 4...
Journal Articles
Journal:
Journal of Cell Science
J Cell Sci (1990) 96 (1): 63–69.
Published: 1 May 1990
...Angela Anastasi; Cherryl Hunt; Howard Stebbings ABSTRACT The ovaries of hemipteran insects contain massive microtubule-based translocation channels known as nutritive tubes, linking nurse cells to the developing oocytes. Translocation, which is in a retrograde direction along the nutritive tube...
Journal Articles
Journal:
Journal of Cell Science
J Cell Sci (1989) 94 (4): 695–701.
Published: 1 December 1989
...Elizabeth S. Sztul; Tom W. Chu; Arnold W. Strauss; Leon E. Rosenberg ABSTRACT We have identified translocational intermediates generated during import of precursor proteins into the mitochondrial matrix and have characterized their association with mitochondrial membranes. Partially translocated...
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