... showed an increased number of SOX2-positive proliferative stem cells and enhanced basal neuronal lineage maturation. In addition, computational gene perturbation analysis revealed 87 transcription factors that might contribute to neurogenesis, among which was SOX2. Conditional knockout of Cxcr4 did...

...Tanja Müller; Marina Reichlmeir; Ann-Christin Hau; Ilka Wittig; Dorothea Schulte ABSTRACT Tight control over transcription factor activity is necessary for a sensible balance between cellular proliferation and differentiation in the embryo and during tissue homeostasis by adult stem cells...

... activation of the other Dictyostelium MAPK (Erk1) in a secondary response, as well as phosphorylating (marked by ‘ P ’) and mediating translocation of the transcription factor GtaC. Other signaling components shown are adenylyl cyclase (AC), RegA cAMP-specific phosphodiesterase (PDE), conventional MAPK...

... easy and precise visualization of the transcriptional activity of endogenous p53. p53 Cell biology Transcription factor Cell cycle Cell death Fluorescent reporter system Japan Society for the Promotion of Science http://dx.doi.org/10.13039/501100001691 20H02873...

... role in stem cell function. However, the mechanisms that modulate the function of ECM regulators in the stem cell niche are understudied. Here, we explored the role of the transcription factor (TF) ETS-1, which is expressed in the cathepsin-positive cell population, in regulating the expression...

...-stimulated translocation of the GATA transcription factor GtaC as indicated by the distribution of a GFP–GtaC reporter. Erk2 was also found to be essential for the translocation of GtaC in response to external folate, a foraging signal that directs the chemotaxis of amoeba to bacteria. Erk1, the only other...

...Radiya G. Ali; Helen M. Bellchambers; Nicholas Warr; Jehangir N. Ahmed; Kristen S. Barratt; Kieran Neill; Koula E. M. Diamand; Ruth M. Arkell ABSTRACT Zinc finger of the cerebellum (Zic) proteins act as classic transcription factors to promote transcription of the Foxd3 gene during neural crest...

...Veer I. P. Keizer; Stefano Coppola; Adriaan B. Houtsmuller; Bart Geverts; Martin E. van Royen; Thomas Schmidt; Marcel J. M. Schaaf ABSTRACT Transcription factor mobility is a determining factor in the regulation of gene expression. Here, we have studied the intranuclear dynamics...

... regulation in neuroendocrine differentiated cells. This point mutation altered the promoter's regulatory mechanism by discrete changes in transcription factor TR occupancy and altered enrichment of the repressive chromatin modification of histone-3-lysine-9-trimethyl (H3K9Me3). Insights relating...

... RNAs and their association with nuclear stress bodies (nSBs) of unknown function. Besides association with the Sat3 transcript, the nSBs are also known to recruit the transcription factors HSF1 and CREBBP, and several RNA-binding proteins, including the splicing factor SRSF1. We demonstrate here...

... activation are largely unknown. Recently, we identified a wave of immediate early transcription factors that are upregulated in human lymphatic endothelial cells (LECs) within the first 30 to 80 min after VEGFR-3 activation. Expression of these transcription factors must be regulated by additional pre...

... of the ATF6 gene by Runx2 and its repression by the Sox6 transcription factor. Runx2 and Sox6 act through their respective binding elements on the ATF6 gene. When overexpressed, ATF6 and ATF6a intensify chondrogenesis; our studies demonstrate that under the stimulation of ATF6 and ATF6a, chondrocytes tend...

...Soshi Kanemoto; Yasuhiro Kobayashi; Teruhito Yamashita; Takeshi Miyamoto; Min Cui; Rie Asada; Xiang Cui; Kenta Hino; Masayuki Kaneko; Tomoko Takai; Koji Matsuhisa; Naoyuki Takahashi; Kazunori Imaizumi ABSTRACT Luman (also known as CREB3) is a type-II transmembrane transcription factor belonging...

... spectroscopy, to provide a quantitative model of the action of this transcription factor. This approach enabled us to distinguish two types of AR–DNA binding: very brief interactions, in the order of a few hundred milliseconds, and hormone-induced longer-lasting interactions, with a characteristic binding time...

...Hiroshi Senoo; Tsuyoshi Araki; Masashi Fukuzawa; Jeffrey G. Williams Summary MrfA, a transcription factor that regulates Dictyostelium prestalk cell differentiation, is an orthologue of the metazoan myelin gene regulatory factor (MRF) proteins. We show that the MRFs contain a predicted...

... fluorescence in situ hybridization coupled to ‘high-precision’ localization, we show that promoters binding the same transcription factors and responding to the same signaling pathways tend to be co-transcribed in the same transcription factories. We go on to suggest how such spatial co-association might drive...

...Yoko Takigawa; Kenji Hata; Shuji Muramatsu; Katsuhiko Amano; Koichiro Ono; Makoto Wakabayashi; Akio Matsuda; Kenji Takada; Riko Nishimura; Toshiyuki Yoneda Sox9 is an essential transcription factor for chondrogenesis by regulating the expression of chondrogenic genes. However, its regulatory...

...José-Manuel Mingot; Sonia Vega; Beatriz Maestro; Jesús M. Sanz; M. Angela Nieto Snail proteins are C 2 H 2 class zinc finger transcription factors involved in different processes during embryonic development, as well as in several adult pathologies including cancer and organ fibrosis...

...Virginie Firlej; Franck Ladam; Guillaume Brysbaert; Patrick Dumont; François Fuks; Yvan de Launoit; Arndt Benecke; Anne Chotteau-Lelievre Pea3 and Erm are transcription factors expressed in normal developing branching organs such as the mammary gland. Deregulation of their expression is generally...

..., transcription factor dynamics and the kinetics of RNA synthesis. These studies show how imaging gene expression in single living cells can reveal new regulatory mechanisms. They also expand our understanding of the role of chromatin and RNA dynamics in modulating cellular responses to developmental...