...Rodolpho Ornitz Oliveira Souza; Kylie N. Jacobs; Peter S. Back; Peter J. Bradley; Gustavo Arrizabalaga ABSTRACT The single mitochondrion of Toxoplasma gondii is highly dynamic, being predominantly in a peripherally distributed lasso-shape in intracellular parasites and collapsed in extracellular...

... such as NAT8. However, the involvement of the ER acetyl-CoA pool in acetylation of ER-transiting proteins in Apicomplexa is unknown. Here, we identified homologs of AT1 and NAT8 in Toxoplasma gondii and Plasmodium berghei parasites. Proteome-wide analyses revealed widespread N-terminal acetylation of secreted...

... diverse and include (1) formation of moving junctions that drive parasites into host cells, as for the protozoans Toxoplasma gondii and Plasmodium spp., (2) subversion of endocytic pathways used by the host cell to repair PM, as for Trypanosoma cruzi and Leishmania , (3) induction of phagocytosis...

... and the establishment of an intracellular lifestyle. Little is known about rhoptry biogenesis and recycling during parasite division. In Toxoplasma gondii , positioning of rhoptries involves the armadillo repeats only protein (ARO) and myosin F (MyoF). Here, we show that two ARO partners, ARO-interacting protein (AIP...

... recovery after photobleaching (FRAP) and mEos2 photoactivation to monitor the dynamics of IMC biogenesis and turnover during the replication of Toxoplasma gondii tachyzoites. These studies reveal that the formation of the T. gondii IMC involves two distinct steps – de novo assembly during daughter IMC...

...Katherine S. Masek; Jim Fiore; Michael Leitges; Shi-Fang Yan; Bruce D. Freedman; Christopher A. Hunter In healthy hosts, acute infection with the opportunistic pathogen Toxoplasma gondii is controlled by innate production of IL-12, a key cytokine crucial for the development of protective immunity...

...Robert E. Molestina; Anthony P. Sinai Activation of NF-κB by the intracellular pathogen Toxoplasma gondii is associated with the localization of phosphorylated IκBα to the parasitophorous vacuole membrane (PVM). This is mediated by a parasite-derived IκB kinase (TgIKK) activity and is independent...

... is targeting of the steps that lead to IκB degradation, a major regulatory checkpoint of this pathway. The data presented here demonstrate that infection of mammalian cells with Toxoplasma gondii results in the activation of IKK and degradation of IκB. However, despite initiation of these hallmarks of NF-κB...

... between the ER and golgi complex in the intracellular parasite Toxoplasma gondii. J. Cell Sci. 112 , 2631 -2638. Harlow, E. and Lane, D. ( 1988 ). Antibodies: A Laboratory Manual . Cold Spring Harbor, NY: Cold Spring Harbor Laboratory. Haverkos, H. W. ( 1987 ). Assessment of therapy...

...T. Matthew Payne; Robert E. Molestina; Anthony P. Sinai Mammalian cells infected with the protozoan parasite Toxoplasma gondii are resistant to many apoptotic stimuli transmitted along both the mitochondrial and death receptor pathways. Apoptosis, and its inhibition in infected cells, was examined...

...Robert E. Molestina; T. Matthew Payne; Isabelle Coppens; Anthony P. Sinai Mammalian cells infected with Toxoplasma gondii are resistant to apoptosis induced by a variety of stimuli. We have demonstrated that the host transcription factor NF-κB plays a pivotal role in the T.-gondii -mediated...

... Toxoplasma gondii Micronemes Epidermal growth factor-like domain Secretion Processing Escorter T. gondii belongs to the phylum Apicomplexa, which includes important pathogens of human and animals. These obligate intracellular parasites share many common structural and functional features...

...Stefan Goebel; Uwe Gross; Carsten G. K. Lüder The obligate intracellular protozoan parasite Toxoplasma gondii has been shown to protect different cell types from apoptosis induced by a variety of pro-apoptotic treatments. However, the precise cell biological mechanisms of this inhibition remained...

...Isao Kimata; Kazuyuki Tanabe ABSTRACT Monoclonal antibodies against Toxoplasma gondii were prepared to characterize antigens of the parasite. Immunoperoxidase staining of parasites fixed with paraformaldehyde and glutaraldehyde (PFAGA) followed by Triton X-100 treatment showed that the antibody...