...Rodolpho Ornitz Oliveira Souza; Kylie N. Jacobs; Peter S. Back; Peter J. Bradley; Gustavo Arrizabalaga ABSTRACT The single mitochondrion of Toxoplasma gondii is highly dynamic, being predominantly in a peripherally distributed lasso-shape in intracellular parasites and collapsed in extracellular...

... such as NAT8. However, the involvement of the ER acetyl-CoA pool in acetylation of ER-transiting proteins in Apicomplexa is unknown. Here, we identified homologs of AT1 and NAT8 in Toxoplasma gondii and Plasmodium berghei parasites. Proteome-wide analyses revealed widespread N-terminal acetylation of secreted...

... diverse and include (1) formation of moving junctions that drive parasites into host cells, as for the protozoans Toxoplasma gondii and Plasmodium spp., (2) subversion of endocytic pathways used by the host cell to repair PM, as for Trypanosoma cruzi and Leishmania , (3) induction of phagocytosis...

... and the establishment of an intracellular lifestyle. Little is known about rhoptry biogenesis and recycling during parasite division. In Toxoplasma gondii , positioning of rhoptries involves the armadillo repeats only protein (ARO) and myosin F (MyoF). Here, we show that two ARO partners, ARO-interacting protein (AIP...

... recovery after photobleaching (FRAP) and mEos2 photoactivation to monitor the dynamics of IMC biogenesis and turnover during the replication of Toxoplasma gondii tachyzoites. These studies reveal that the formation of the T. gondii IMC involves two distinct steps – de novo assembly during daughter IMC...

...Katherine S. Masek; Jim Fiore; Michael Leitges; Shi-Fang Yan; Bruce D. Freedman; Christopher A. Hunter In healthy hosts, acute infection with the opportunistic pathogen Toxoplasma gondii is controlled by innate production of IL-12, a key cytokine crucial for the development of protective immunity...

...Robert E. Molestina; Anthony P. Sinai Activation of NF-κB by the intracellular pathogen Toxoplasma gondii is associated with the localization of phosphorylated IκBα to the parasitophorous vacuole membrane (PVM). This is mediated by a parasite-derived IκB kinase (TgIKK) activity and is independent...

... is targeting of the steps that lead to IκB degradation, a major regulatory checkpoint of this pathway. The data presented here demonstrate that infection of mammalian cells with Toxoplasma gondii results in the activation of IKK and degradation of IκB. However, despite initiation of these hallmarks of NF-κB...

... for correspondence (e-mail: [email protected] ) 7 7 2003 © The Company of Biologists Limited 2003 2003 Apicomplexa Toxoplasma gondii Mitochondrion Apicoplast Targeting Presequence Protein trafficking in eukaryotic cells is a complex process that involves many different signal...

...T. Matthew Payne; Robert E. Molestina; Anthony P. Sinai Mammalian cells infected with the protozoan parasite Toxoplasma gondii are resistant to many apoptotic stimuli transmitted along both the mitochondrial and death receptor pathways. Apoptosis, and its inhibition in infected cells, was examined...

...Robert E. Molestina; T. Matthew Payne; Isabelle Coppens; Anthony P. Sinai Mammalian cells infected with Toxoplasma gondii are resistant to apoptosis induced by a variety of stimuli. We have demonstrated that the host transcription factor NF-κB plays a pivotal role in the T.-gondii -mediated...

... Toxoplasma gondii Micronemes Epidermal growth factor-like domain Secretion Processing Escorter The exact cleavage site at the N-terminus of TgMIC6 was determined by mass spectrometry analysis after immunoprecipitation of MIC6Ty and purification of the 35 kDa species by SDS-PAGE. The protein...

...Stefan Goebel; Uwe Gross; Carsten G. K. Lüder The obligate intracellular protozoan parasite Toxoplasma gondii has been shown to protect different cell types from apoptosis induced by a variety of pro-apoptotic treatments. However, the precise cell biological mechanisms of this inhibition remained...

...Amy DeRocher; Christopher B. Hagen; John E. Froehlich; Jean E. Feagin; Marilyn Parsons ABSTRACT Apicomplexan parasites possess a plastid-like organelle called the apicoplast. Most proteins in the Toxoplasma gondii apicoplast are encoded in the nucleus and imported post-translationally. T. gondii...

...M. Ding; C. Clayton; D. Soldati ABSTRACT The intracellular protozoan parasite Toxoplasma gondii , like all members of the phylum Apicomplexa, is known to possess many organelles: in addition to mitochondria and the compartments of the secretory pathway, there is a reduced chloroplast...

...Michael K. Shaw; Helen L. Compton; David S. Roos; Lewis G. Tilney ABSTRACT We have used drugs to examine the role(s) of the actin and microtubule cytoskeletons in the intracellular growth and replication of the intracellular protozoan parasite, Toxoplasma gondii . By using a 5 minute infection...

...Kristin M. Hager; Boris Striepen; Lewis G. Tilney; David S. Roos ABSTRACT Morphological examination of the highly polarized protozoan parasite Toxoplasma gondii suggests that secretory traffic in this organism progresses from the endoplasmic reticulum to the Golgi apparatus using the nuclear...

...Corinne Mercier; Marie-France Cesbron-Delauw; L. David Sibley ABSTRACT The Toxoplasma gondii protein GRA2 is secreted into the parasite-containing vacuole where it is rapidly and specifically targeted to a network of membranous tubules that connect with the vacuolar membrane. To examine...

... apicomplexan parasite, Toxoplasma gondii . A polyclonal antibody was generated against an expressed polypeptide of molecular mass 27,000, based on part of the deduced sequence of this myosin. The antibody reacted with the cognate antigen and with a component of the total parasite protein on immunoblots...

...Frank Seeber; Jean-François Dubremetz; John C. Boothroyd ABSTRACT We have genetically engineered Toxoplasma gondii so that its major surface antigen SAG1 is anchored by a human transmembrane domain (SAG1-TM) instead of its natural GPI anchor (SAG1-GPI) in order to initiate studies to address...