...Valerie Panneels; Ana Diaz; Cornelia Imsand; Manuel Guizar-Sicairos; Elisabeth Müller; Anne Greet Bittermann; Takashi Ishikawa; Andreas Menzel; Andres Kaech; Mirko Holler; Christian Grimm; Gebhard Schertler ABSTRACT Ptychographic hard X-ray computed tomography (PXCT) is a recent method allowing...

.... 3D electron microscopy Tomography Metal-tagging transmission electron microscopy Clonable tag Viral replication factory The life of the cell depends on compartmentalized interactions of macromolecular assemblies. Shortly after synthesis, proteins must travel from ribosomes...

... adopted techniques for characterising the 3D structure of non-calcified tissues and biomaterials include optical and electron microscopy of serial sections and sectioned block faces, and the visualisation of intact samples by confocal microscopy or electron tomography. As an alternative...

... and electron tomography to gain insight into the spatio-temporal organization of lipid droplets during cellular growth in the yeast Saccharomyces cerevisiae . This analysis revealed a high level of organization of the subcellular positioning of lipid droplets in individual cells, their directed migration...

... were used for tomography. Images were digitized and models constructed using Midas and Imod software(http://bio3d.colorado.edu). Images were aligned and contours drawn on specific cellular structures. The contours from a stack of serial sections were arranged into objects and meshed into 3D structures...

...Christopher L. Woodcock; Bruce F. McEwen; Joachim Frank ABSTRACT Electron-microscope tomography has been used to reconstruct isolated, negatively stained chromatin fibers from Necturus maculosus erythrocytes. Tilt series micrographs from +70° to —70° at 5° intervals were obtained, allowing...