...Ariane Biquand; Simone Spinozzi; Paola Tonino; Jérémie Cosette; Joshua Strom; Zaher Elbeck; Ralph Knöll; Henk Granzier; William Lostal; Isabelle Richard ABSTRACT Titin is a giant sarcomeric protein that is involved in a large number of functions, with a primary role in skeletal and cardiac...

...Zsolt Mártonfalvi; Pasquale Bianco; Marco Linari; Marco Caremani; Attila Nagy; Vincenzo Lombardi; Miklós Kellermayer ABSTRACT Titin is a giant elastomeric muscle protein that has been suggested to function as a sensor of sarcomeric stress and strain, but the mechanisms by which it does so...

... with the Z-disk is mediated by Z-disk titin. Here, we unravel the molecular basis for the unusual localization of obscurin, a Z-disk-associated protein, to the M-band, where its invertebrate analogue UNC-89 is also localized. The first three domains of the N-terminus of obscurin bind to the most C-terminal...

...Lacramioara Fabian; Xuequin Xia; Deepa V. Venkitaramani; Kristen M. Johansen; Jørgen Johansen; Deborah J. Andrew; Arthur Forer Titin, the giant elastic protein found in muscles, is present in spindles of crane-fly and locust spermatocytes as determined by immunofluorescence staining using three...

...Hanny Musa; Stephen Meek; Mathias Gautel; Dianna Peddie; Andrew J. H. Smith; Michelle Peckham Titin, a multifunctional protein that stretches from the Z-disk to the M-band in heart and skeletal muscle, contains a kinase domain, phosphorylation sites and multiple binding sites for structural...

... out a two-hybrid screen with a human skeletal-muscle cDNA library, using the Ig-fold domain of lamin A as bait. The C-terminal region of titin was recovered twice. Previous investigators showed that nuclear isoforms of titin are essential for chromosome condensation during mitosis. Our titin fragment...

...Attila Nagy; Paola Cacciafesta; László Grama; András Kengyel; András Málnási-Csizmadia; Miklós S. Z. Kellermayer Parts of the PEVK (Pro-Glu-Val-Lys) domain of the skeletal muscle isoform of the giant intrasarcomeric protein titin have been shown to bind F-actin. However, the mechanisms...

... the giant protein, titin), microtubules and/or nuclear factors. We investigated the role of MURF-2, the least-characterized family member, in primary cultures of embryonic chick skeletal and cardiac myocytes. MURF-2 is detected as two species (∼55 kDa and ∼60 kDa) in embryonic muscle, which are down...

...Gaynor Miller; Hanny Musa; Matthias Gautel; Michelle Peckham Titin is the largest protein known, and is essential for organising muscle sarcomeres. It has many domains with a variety of functions, and stretches from the Z-line to the M-line in the muscle sarcomere. Close to the M-line, titin...

... and are transiently present in the myofibril. We show here that the novel tubulin-associated RING/B-box protein MURF2 associates transiently with microtubules, myosin and titin during sarcomere assembly. During sarcomere assembly, MURF2 first associates with microtubules at the exclusion of tyrosinated tubulin...

...Veronika Person; Sawa Kostin; Keisuke Suzuki; Siegfried Labeit; Jutta Schaper ABSTRACT An essential role of titin as a molecular ruler in sarcomerogenesis has been frequently discussed. In this study, we tested the hypothesis that the expression of titin is a prerequisite for thick filament...

... precursors) and, a short time later, into definitive I-Z-I bands. By double-staining the growth tips of transfected myotubes expressing (a) MYC-tagged s-α-actinins (MYC/s-α-actinins) or (b) green fluorescent protein-tagged titin cap (GFP/T-cap) with antibodies against MYC and I-Z-I band proteins, we found...

...Mathias Gautel; David Goulding; Belinda Bullard; Klaus Weber; Dieter O. Fürst ABSTRACT The giant sarcomeric protein titin (also described as connectin) is composed mainly of immunoglobulin (Ig)-like and fibronectin type III (fn3)-like domains arranged consecutively. At both ends of the molecule...

...Magdolna G. Sebestyén; Jon A. Wolff; Marion L. Greaser ABSTRACT Titin is an approximately 3 MDa protein that spans from the M- to the Z-line in the sarcomeres of vertebrate striated muscle. The protein is presumably encoded by unusually large mRNAs of 70-80 kb. Although titin has been studied...

...Peter F. M. van der Ven; Gert Schaart; Huib J. E. Croes; Paul H. K. Jap; Leo A. Ginsel; Frans C. S. Ramaekers ABSTRACT Differentiating human skeletal muscle cell cultures were used to study the association of titin with other sarcomeric and cytoskeletal proteins during myofibrillo-genesis. Several...

... ). Association of titin and myosin heavy chain in developing muscle . Proc. Nat. Acad. Sci. USA 89 , 7496 – 7500 . 10.1073/pnas.89.16.7496 Isaacs , W. B. , Kim , I. S. Struve , A. and Fulton , A. B. ( 1989 ). Biosynthesis of titin in cultured skeletal muscle cells . J. Cell...

...A. Soteriou; M. Gamage; J. Trinick ABSTRACT A simple solid-phase binding assay was used to screen for interactions that the giant myofibrillar protein titin makes with other sarcomeric proteins. The titin used in the tests was purified by a modified procedure that results in isolation of ∼20 mg...

... filament overlap: they occupy the gap between the tips of thick and thin filaments. To test whether the gap filaments are part of the titin-filament system, we employed monoclonal antibodies to titin (T-ll, Sigma) and observed the location of the epitope at a series of sarcomere lengths. At resting...

... molecular weight of 600 000 and appear in oriented specimens as flexible thin rods with a length around 240–250 nm. The circular dichroism spectrum of the Ascaris protein is dominated by β-structure. The proteins react with antibodies to insect mini-titin and also with antibodies raised against peptides...

... structure of the molecule is confirmed by rotary shadowing. The flexible rods have a uniform diameter of 3-4 nm and an average length of 260 nm. Polyclonal antibodies show crossreactivity between Hmp II and its putative precursor Hmp I. We discuss the similarities and differences between the larger titin l...