...Sandra Citi; Michael Fromm; Mikio Furuse; Lorenza González-Mariscal; Asma Nusrat; Sachiko Tsukita; Jerrold R. Turner ABSTRACT Tight junctions (TJs) are specialized regions of contact between cells of epithelial and endothelial tissues that form selective semipermeable paracellular barriers...

... mucin MUC13 is an abundant intestinal glycoprotein with important functions for mucosal maintenance that are not yet completely understood. We demonstrate that in human intestinal epithelial monolayers, MUC13 localized to both the apical surface and the tight junction (TJ) region on the lateral membrane...

... previously developed a method that we named the zinc-based ultrasensitive microscopic barrier assay (ZnUMBA), which overcomes these limitations, allowing for detection of local tight junction leaks with high spatiotemporal resolution. Here, we present expanded applications for ZnUMBA. ZnUMBA can be used...

... remaining sufficiently plastic to allow events such as cytokinesis to take place is a key biological problem. In vertebrates, the remodelling and reinforcement of adherens junctions maintains epithelial integrity during cytokinesis. The involvement of tight junctions in cell division, however, has remained...

.... Failure of these processes can result in serious developmental anomalies. Although tight junction assembly is crucial to epithelial polarization, the contribution of specific tight junction proteins to lumenogenesis is undefined. Here, we show that ZO-1 (also known as TJP1) is necessary for the formation...

...Imogen A. Elsum; Claire Martin; Patrick O. Humbert Summary The crucial role the Crumbs and Par polarity complexes play in tight junction integrity has long been established, however very few studies have investigated the role of the Scribble polarity module. Here, we use MCF10A cells, which fail...

... in polarised epithelial tissues, but its function in these tissues and the relationship with the disorder are unknown. Here we show colocalisation of POF1B with markers of both adherens and tight junctions in human jejunum. The tight junction localisation was maintained by the human POF1B stably expressed...

... form adherens junctions (AJs) but completely lack tight junctions (TJs) – fully activated SRF. By contrast, AGS gastric adenocarcinoma epithelial cells, which form TJs but are deficient in E-cadherin, and therefore also in AJs, failed to activate SRF. The introduction of wild-type E-cadherin in AGS...

...Senye Takahashi; Noriko Iwamoto; Hiroyuki Sasaki; Masato Ohashi; Yukako Oda; Shoichiro Tsukita; Mikio Furuse The structural continuity of tight junctions (TJs) is consistently maintained even when epithelial cells divide and move within the cellular sheet. This process is associated with dynamic...

...Ernesto Sabath; Hideyuki Negoro; Sarah Beaudry; Manuel Paniagua; Susanne Angelow; Jagesh Shah; Nicholas Grammatikakis; Alan S. L. Yu; Bradley M. Denker The polarized functions of epithelia require an intact tight junction (TJ) to restrict paracellular movement and to separate membrane proteins...

...Christina M. Van Itallie; Jennifer Holmes; Arlene Bridges; Jody L. Gookin; Maria R. Coccaro; William Proctor; Oscar R. Colegio; James M. Anderson Epithelial tight junctions contain size- and charge-selective pores that control the paracellular movement of charged and noncharged solutes. Claudins...

.... Here, we report on shroom2, a submembranous PDZ domain-containing protein that is associated with the tight junctions in multiple embryonic and adult epithelia. Shroom2 directly interacts with the C-terminal MyTH4-FERM domain of myosin VIIa and with F-actin. In addition, a shroom2 fragment containing...

...Takako Ooshio; Naoyuki Fujita; Akio Yamada; Tatsuhiro Sato; Yuichi Kitagawa; Ryoko Okamoto; Shinsuke Nakata; Ayaka Miki; Kenji Irie; Yoshimi Takai Par-3 is a cell-polarity protein that regulates the formation of tight junctions (TJs) in epithelial cells, where claudin is a major cell-cell adhesion...

... lines of Caco2 to clarify its role in epithelial morphogenesis. In PATJ knockdown cells, Pals1 (a Stardust homologue) is no longer associated with tight junctions whereas Crumbs3 (Crb3) is accumulated into a compartment spatially close to the apical membrane and related to early endosomes. Furthermore...

...-forming proteins of the tight junction, are palmitoylated and whether this modification has functional implications for the tight-junction barrier. Claudin-14, like other members of the claudin family, contains membrane-proximal cysteines following both the second and the fourth transmembrane domains...

...Christoph Rahner; Masayuki Fukuhara; Shaomin Peng; Shota Kojima; Lawrence J. Rizzolo A culture model has been established to study the gradual development of tight junctions during the embryogenesis of the chick retinal pigment epithelium. This study asks how closely the culture model reflects...

... junctions. The dramatic morphological changes observed in apoptotic epithelial cells were ascribed at least in part to the specific fragmentation of components of adherens junctions and desmosomes. Little, however, is known about tight junctions during apoptosis. We have found that after induction...

...Ching-hang Wong; Dolores D. Mruk; Wing-yee Lui; C. Yan Cheng An in vivo model was used to investigate the regulation of tight junction (TJ) dynamics in the testis when adult rats were treated with CdCl 2 . It was shown that the CdCl 2 -induced disruption of the blood-testis barrier (BTB) associated...

... enriched at tight junctions. The recent identification of extracellular ligands and intracellular binding proteins for JAMs suggests two functions for JAMs. JAMs associate through their extracellular domains with the leukocyte β2 integrins LFA-1 and Mac-1 as well as with the β1 integrin α4β1. All three...

... of patients with colorectal carcinoma. Progastrin induced the dissociation of both tight junction (TJ) and adherens junction (AJ) complexes in IMGE-5 cells. In progastrin-secreting DLD-1 human colorectal carcinoma cells, expression of an antisense gastrin construct restored membrane localisation of zonula...