...Lam Ngoc Thao Nguyen; Lam Nhat Nguyen; Juan Zhao; Madison Schank; Xindi Dang; Dechao Cao; Sushant Khanal; Xiao Y. Wu; Yi Zhang; Jinyu Zhang; Shunbin Ning; Ling Wang; Mohamed El Gazzar; Jonathan P. Moorman; Zhi Q. Yao ABSTRACT We investigated the role of telomerase and telomere repeat-binding factor...

... detectable telomerase activity . EMBO J.   14 , 4240 - 4248 . 10.1002/j.1460-2075.1995.tb00098.x Bryan , T. M. , Englezou , A. , Dalla-Pozza , L. , Dunham , M. A. and Reddel , R. R. ( 1997 ). Evidence for an alternative mechanism for maintaining telomere length in human tumors...

...Juyeong Hong; Ji Hoon Lee; In Kwon Chung ABSTRACT Telomerase is a ribonucleoprotein enzyme that is required for the maintenance of telomere repeats. Although overexpression of telomerase in normal human somatic cells is sufficient to overcome replicative senescence, the ability of telomerase...

...Mengfan Tang; Yujing Li; Yi Zhang; Yuxi Chen; Wenjun Huang; Dan Wang; Arthur J. Zaug; Dan Liu; Yong Zhao; Thomas R. Cech; Wenbin Ma; Zhou Songyang ABSTRACT Most human cancers depend on the telomerase to maintain telomeres; however, about 10% of cancers are telomerase negative and utilize...

...Sicong Zeng; Lvjun Liu; Yi Sun; Pingyuan Xie; Liang Hu; Ding Yuan; Dehua Chen; Qi Ouyang; Ge Lin; Guangxiu Lu ABSTRACT High telomerase activity is a characteristic of human embryonic stem cells (hESCs), however, the regulation and maintenance of correct telomere length in hESCs is unclear...

...Yasmin D'Souza; Catherine Lauzon; Tsz Wai Chu; Chantal Autexier Summary Telomerase is a ribonucleoprotein consisting of a catalytic subunit, the telomerase reverse transcriptase (TERT), and an integrally associated RNA that contains a template for the synthesis of short repetitive G-rich DNA...

...Jeeyun Chung; Prabhat Khadka; In Kwon Chung Sustained cell proliferation requires telomerase to maintain functional telomeres that are essential for chromosome integrity and protection. Although nuclear import of telomerase transcriptase (hTERT) is required for telomerase activity to elongate...

...Robin C. B. Bairley; Gina Guillaume; Leticia R. Vega; Katherine L. Friedman Telomerase is a ribonucleoprotein complex that is required for maintenance of linear chromosome ends (telomeres). In yeast, the Est2 protein reverse transcribes a short template region of the TLC1 RNA using the chromosome...

... induces early senescence associated with increased DNA-damage signaling and that telomerase extends HGPS cellular lifespan. We demonstrate that telomerase extends HGPS cellular lifespan by decreasing progerin-induced DNA-damage signaling and activation of p53 and Rb pathways that otherwise mediate...

...Zhu-Hong Li; Rebecca L. Tomlinson; Rebecca M. Terns; Michael P. Terns The core components of telomerase are telomerase RNA (TR) and telomerase reverse transcriptase (TERT). In vertebrate cells, TR and TERT have been reported to associate with intranuclear structures, including Cajal bodies...

..., as it is becoming increasingly evident that, although unwanted DNA repair at telomeres must be avoided at all costs, the molecular players involved in recognition, signaling and repair of DNA damage might also serve to protect telomeres. Telomeres Telomerase Shelterin ALT Aging Recombination Genome...

...Wonkyung Oh; Jaewang Ghim; Eun-Woo Lee; Mi-Ran Yang; Eui Tae Kim; Jin-Hyun Ahn; Jaewhan Song Maintaining proper telomere length requires the presence of the telomerase enzyme. Here we show that telomerase reverse transcriptase (TERT), a catalytic component of telomerase, is recruited...

...Jian Lin; Rui Jin; Bin Zhang; Hao Chen; Yun Xiu Bai; Ping Xun Yang; Su Wen Han; Yao Hua Xie; Pei Tang Huang; Cuifen Huang; Jun Jian Huang Telomerase maintains telomere length and has been implicated in both aging and carcinogenesis of human cells. This enzyme is a specialized ribonucleoprotein (RNP...

...Shaheda Ahmed; João F. Passos; Matthew J. Birket; Tina Beckmann; Sebastian Brings; Heiko Peters; Mark A. Birch-Machin; Thomas von Zglinicki; Gabriele Saretzki Telomerase is a ribonucleoprotein that counteracts telomere shortening and can immortalise human cells. There is also evidence...

... and investigate the signals required for their proliferation and survival. Clonally proliferated CSCs express stem cell antigen-1 (Sca-1) with embryonic stem (ES) cell-like and mesenchymal cell-like characteristics and are associated with telomerase reverse transcriptase (TERT). Using a transgene that expresses...

...Ying-Li Wu; Charles Dudognon; Eric Nguyen; Josette Hillion; Frédéric Pendino; Ilona Tarkanyi; Janos Aradi; Michel Lanotte; Jian-Hua Tong; Guo-Qiang Chen; Evelyne Ségal-Bendirdjian The involvement of telomerase in cellular immortalization and senescence has often been assessed by means...

... heterogeneity, and ruled out telomeric end-to-end fusions as the source of the long TRFs. The latter was also confirmed by Bal31 digestion of genomic DNA. Similarly, upregulation of telomerase could not account for the appearance of long TRFs, as oxidative stress induced a rapid and sustained decrease...

... that an additional 12 out of 12 telomeric regions examined also display metaphase singlet signals in pre-senescent cells, and that excess telomere-metaphase singlets also occur in earlier passage cells harvested from elderly individuals. In cancer cell lines expressing telomerase and in pre-senescent fibroblasts...

...Matthias Möllenbeck; Jan Postberg; Katrin Paeschke; Michael Rossbach; Franziska Jönsson; Hans J. Lipps Telomere replication of eukaryotic chromosomes is achieved by a specialized enzyme, the telomerase. Although the biochemistry of end-replication is well understood, little is known about...

... not proliferate indefinitely but reached a second proliferative lifespan barrier, termed M int , that could be bypassed by forced production of telomerase in post-M1 E6-producing cells. The conclusions from these studies are that: (1) replicative senescence in Werner-syndrome fibroblasts is a telomere-induced p53...