... to the depolymerising drug nocodazole or the microtubule-stabilising drug taxol, which is quantitated by mass spectrometry. Our approach is benchmarked by co-segregation of tubulin and previously established microtubule-binding proteins. We then identify several novel candidate microtubule-binding proteins, from which...

... chemotherapy forms the mainstay of cancer treatment, and agents that disrupt mitotic spindle assembly – so called `anti-mitotics' – are commonly used to treat a wide variety of cancers. Traditional anti-mitotic agents include the microtubule toxins such as taxol, other taxanes and the vinca alkaloids, all...

... ; [email protected]) © The Company of Biologists Limited 2009 2009 Calcium Gene transcription Ins P 3 Nucleus Paclitaxel Ryanodine Signalling Spindle checkpoint Taxol Calcium (Ca 2+) is a key player in signal transduction. It modulates diverse cellular activities ranging from...

... to substantially reduce the level of ATP in living cells and we used Taxol to halt microtubule dynamics. Both treatments slowed the dynamics of 5×GFP-EMTB speckles observed by FSM. We also used fluorescence recovery after photobleaching (FRAP) to quantify the half-time of binding and dissociation of the 5×GFP-EMTB...

... conditions. However, stabilization of microtubules by taxol did not affect the proliferation of mitochondria during the cell cycle, yet a prolonged incubation of cells with taxol induced an abnormal accumulation of mitochondria in cells arrested in G 2 /M phase of cell cycle. Taxol-induced accumulation...

... 1999 by Company of Biologists 1999 Fluorescent speckle microscopy Taxol Carcinoma cell Non-motor MAP Cells elaborate characteristic assemblies of microtubules (MTs) to carry out cell motility, transport and positioning of vesicles and organelles, segregation of chromosomes...

...-specific drugs, colcemid and taxol, indicate that the organization of this cortex is under microtubular control. * Author for correspondence at present address: Cancer Research Center, Kashirscoye, Shosse 24, Moscow 115478, Russia (e-mail: [email protected]) 23 03 1999 08 02 1999...

... redistribution of the protein during mitosis, B23 also becomes associated with mitotic spindle poles starting from early prometaphase onwards. Colocalization of B23 with the protein NuMA (Nuclear Mitotic Apparatus protein) was studied in mitotic cells and taxol-arrested cells. During the onset of mitosis, we...

...Patricia M. Vilalta; Liping Zhang; Sarah F. Hamm-Alvarez ABSTRACT To understand how protein phosphorylation modulates cytoskeletal organization, we used immunofluorescence microscopy to examine the effects of okadaic acid, a serine/threonine protein phosphatase inhibitor, and taxol, a microtubule...

..., nocodazole and taxol. Fluctuations in the rate of retraction of the margin were about twice as powerful as fluctuations in the rate of protrusion. High-frequency fluctuations were also apparent in the cell track and in measures of cell spreading, shape and speed. These rapid fluctuations were also all...

... and nontyrosinated alpha-tubulin during mitosis . J. Cell Biol . 102 , 1118 – 1126 . 10.1083/jcb.102.3.1118 Heidemann , S. R. and Gallas , P. T. (1980). The effect of taxol on living eggs of Xenopus laevis . Dev. Biol . 80 , 489 – 494 . 10.1016/0012-1606(80)90421-2 Hennequin...

... is analyzed under a given velocity distribution along its length. Microtubules incubated with taxol after polymerization are measured to be more flexible than those without taxol added. On the other hand, MAPs are shown to increase microtubule stiffness. * Author for correspondence (e-mail: felgner...

... relation to the actin-based cytoskeleton and to ligand-containing endosomes during receptor-mediated endocytosis and the alterations in its structure and disposition by the microtubule pertubant, Taxol. By co-localization studies, we have been able to demonstrate that the microtubules have a significant...

... and thus, MT dynamics are critical for the maximal speed of cell locomotion. This notion was further supported by analogous effects of taxol and vinblastine on cell locomotion: at concentrations that reportedly cause little change in the level of MTs, taxol and vinblastine also dramatically decreased...

...V. B. Dugina; A. Y. Alexandrova; K. Lane; E. Bulanova; J. M. Vasiliev ABSTRACT The effects of the microtubular drugs colcemid and taxol on the morphological changes induced by hepatocyte growth factor/scatter factor (HGF/SF) in MDCK cells were studied. Dynamic changes in the area and shape...

... of this MAP; namely, its unusually tight association with a calcium-insensitive population of MTs in the presence of taxol, was exploited in devising an efficient purification strategy. Because of the MAP’s tenacious association with a stable population of MTs, and because it appeared to contribute...

...Patricia J. Harris; Elise L. Clason ABSTRACT Cytasters were induced in the unfertilized eggs of the sea urchin Lytechinus pictus by two different methods: (1) treatment with hexylene glycol or taxol, which are known to lower the tubulin critical concentration in vitro , but do not activate...

..., and in taxol-treated mitotic cells, that the SPN antigen is involved in organizing the microtubules of the spindle. The 210 kDa protein defined as SPN antigen relocates from the nuclear matrix to the centrosome at prophase, remains associated with the poles at metaphase and anaphase, and dissociates from...

... antibody. This allowed us to compare the subcellular distribution of three kinds of centrosomal antigens in a single cell. Mitotic PtKi cells treated with either nocodazole or taxol included microtubule-containing cytoplasmic foci and parallel bundles of short microtubules at the cell periphery. All...

... to astral microtubule turnover. We treated cells containing monopolar spindles with drugs that affect microtubule turnover, either by promoting the depletion of dynamically unstable astral microtubules (nocodazole and colcemid) or by augmenting their numbers and stability (taxol). Both types of drugs...