... to the actomyosin cytoskeleton, via cytoplasmic proteins. Adhesion depends on the activation of integrins. Talin and kindlin proteins are intracellular activators of integrins that bind to β-integrin cytoplasmic tails. Integrin activation and clustering through extracellular ligands guide the organization...

... of mechanical linkages to mechanochemical switches that enables conversion of forces into biological signals. Mechanotransduction Mechanosensing Motor proteins Cytoskeleton Disease-associated mutations Talin Force-dependent switches Academy of Finland http://dx.doi.org/10.13039...

...Benjamin T. Goult; Nicholas H. Brown; Martin A. Schwartz ABSTRACT Talins are cytoskeletal linker proteins that consist of an N-terminal head domain, a flexible neck region and a C-terminal rod domain made of 13 helical bundles. The head domain binds integrin β-subunit cytoplasmic tails, which...

..., β1 integrin and paxillin. We examined how these interactions enable vimentin to regulate cell migration on collagen. In fibroblasts, depletion of vimentin increased talin-dependent activation of β1 integrin by more than 2-fold. Loss of vimentin was associated with reduction of β1 integrin clustering...

...; Jinhua Wu; Vesa P. Hytönen; Bernhard Wehrle-Haller ABSTRACT Integrin activation and clustering by talin are early steps of cell adhesion. Membrane-bound talin head domain and kindlin bind to the β integrin cytoplasmic tail, cooperating to activate the heterodimeric integrin, and the talin head domain...

...Thomas Bromberger; Liang Zhu; Sarah Klapproth; Jun Qin; Markus Moser ABSTRACT Recruitment and tethering of talin to the plasma membrane initiate the process of integrin activation. Multiple factors including the Rap1 proteins, RIAM (also known as APBB1IP) and PIP 2 bind talin proteins and have been...

...Rolle Rahikainen; Tiina Öhman; Paula Turkki; Markku Varjosalo; Vesa P. Hytönen ABSTRACT Talin protein is one of the key components in integrin-mediated adhesion complexes. Talins transmit mechanical forces between β-integrin and actin, and regulate adhesion complex composition and signaling through...

...Darius Camp; Amanda Haage; Veronika Solianova; William M. Castle; Qinyuan A. Xu; Emily Lostchuck; Benjamin T. Goult; Guy Tanentzapf ABSTRACT Attachment of cells to the extracellular matrix (ECM) via integrins is essential for animal development and tissue maintenance. The cytoplasmic protein Talin...

...Juan Gao; Ming Huang; Jingjing Lai; Kaijun Mao; Peisen Sun; Zhongyuan Cao; Youpei Hu; Yingying Zhang; Marie L. Schulte; Chaozhi Jin; Jian Wang; Gilbert C. White; Zhen Xu; Yan-Qing Ma ABSTRACT Kindlins play an important role in supporting integrin activation by cooperating with talin; however...

...Benjamin Klapholz; Nicholas H. Brown ABSTRACT Talin has emerged as the key cytoplasmic protein that mediates integrin adhesion to the extracellular matrix. In this Review, we draw on experiments performed in mammalian cells in culture and Drosophila to present evidence that talin is the most...

... (FLAP) experiments, we found that the mobility of core FA proteins correlates with their function. Structural proteins such as tensin, talin and vinculin are significantly less mobile in FAs than signaling proteins such as FAK (also known as PTK2) and paxillin. The mobilities of the structural proteins...

... growth in vivo . Thus, Rap1 mediates the effects of increased extracellular tension in multiple ways that are capable of contributing to tumor progression when dysregulated. Once vinculin binds to talin at focal adhesions, it can undergo tension-mediated stretching and straightening, a process...

... is the talin-binding D1 domain, and this appears to require talin-binding activity, as lethality was suppressed by competition with single vinculin-binding sites from talin. Activated Drosophila vinculin triggered the formation of cytoplasmic adhesion complexes through the rod of talin, but independently...

... of H-Ras signaling. H-Ras converted filamentous vimentin into aggregates near the nucleus, where no integrin binding can occur. In addition, an increase in the amount of vimentin filaments accessible to the integrin β3 tail enhanced talin-induced integrin binding to its ligands by inducing integrin...

...Jiafu Liu; Zhengli Wang; Aye Myat Myat Thinn; Yan‐Qing Ma; Jieqing Zhu Studies on the mechanism of integrin inside‐out activation have been focused on the role of β‐integrin cytoplasmic tails, which are relatively conserved and bear binding sites for the intracellular activators including talin...

... that 12 are required in developing adult muscles, consistent with roles downstream of Notch. Focusing on three, Reck , rhea / talin and trio , we verify their expression in adult muscle progenitors and identify Notch-regulated enhancers in each. Full activity of these enhancers requires functional binding...

... and negligible adhesive forces were generated. We then asked whether this adhesive area threshold could be modulated by intracellular pathways known to influence either adhesive force, cytoskeletal tension, or the structural link between the two. Expression of talin- or vinculin-head domains that increase...

... of cytoplasmic proteins, such as talins and kindlins, to the cytoplasmic tail of β-integrins is widely accepted as being the crucial step in integrin activation. By contrast, much less is known with regard to the counteracting mechanism involved in switching integrins into an inactive conformation...

... attachment to the extracellular matrix (ECM), connect to the intracellular environment through the cytoplasmic adapter protein talin. Talin has two essential functions: orchestrating the assembly of the intracellular adhesion complex (IAC), which associates with integrin, and regulating the affinity...

... interactions that are essential for the assembly of this ILK-containing complex at Drosophila muscle attachment sites (MASs). We show that, downstream of integrins, talin plays a decisive role in the recruitment of three proteins: ILK, PINCH and paxillin. The accumulation of ILK at MASs appears to follow...