... that the spindle assembly factor TPX2 regulates astral microtubules. TPX2 in the spindle pole area is activated by GM130 (GOLGA2) on Golgi membranes to promote astral microtubule growth. GM130 relieves TPX2 inhibition by competing for importin α1 (KPNA2) binding. Mitotic phosphorylation of importin α at serine 62...

...Ahmed Abdelbaki; H. Begum Akman; Marion Poteau; Rhys Grant; Olivier Gavet; Giulia Guarguaglini; Catherine Lindon ABSTRACT Activity of AURKA is controlled through multiple mechanisms including phosphorylation, ubiquitin-mediated degradation and allosteric interaction with TPX2. Activity peaks...

... in chromosome alignment and spindle assembly. We found that SAF-A specifically co-immunoprecipitated with the chromosome peripheral protein nucleolin and the spindle regulators Aurora-A and TPX2, indicating that SAF-A is associated with nucleolin and the Aurora-A–TPX2 complex. SAF-A was colocalized with TPX2...

... in G2 and M phases, followed by regulated proteolysis at the end of mitosis. The microtubule-binding protein TPX2 plays a major role in regulating the activity and localisation of Aurora-A in mitotic cells. Here, we report a novel regulatory role of TPX2 and show that it protects Aurora-A from...

... 2010. 2010 Aurora kinase INCENP Mitotic kinases Molecular evolution TPX2 Accurate progression of mitosis (M phase) is ensured by mitotic kinases such as Cdc2, Plk1 and Aurora ( Nigg, 2001 ; Taylor and Peters, 2008 ). Aurora is a family of serine/threonine kinases conserved from yeast...

... contractility provides the impetus for Ran release and, consequently, microtubule assembly is blocked in blebbistatin- and Y27632-treated apoptotic cells. Importantly, the spindle-assembly factor TPX2 (targeting protein for Xklp2), colocalises with apoptotic microtubules, and siRNA silencing of TPX2...

... of centrosomes. * Author for correspondence (e-mail: [email protected] ) 23 8 2006 © The Company of Biologists Limited 2006 2006 Centrosome γ-tubulin Microtubule Nucleation XMAP215 TACC TPX2 Aurora A Microtubules (MTs) are found in all eukaryotic cells and play...

... with mitotic microtubules in vitro and its accumulation at poles requires microtubule integrity and dynamics in vivo. Furthermore, RNA interference-dependent inactivation of TPX2, the major Ran-dependent spindle organizer, abolishes importin β accumulation at poles. Importin β has a functional role in spindle...