Fig. 1. Identification of Cavin1-binding nanobodies. (A) Initial selections of nanobodies interacting with the mouse Cavin1 (mC1)-HR1 domain. GST–mC1-HR1 was used as a bait for His-tagged nanobodies. P, pellet; S, supernatant. (B) Isothermal calorimetry (ITC) thermogram for binding of nanobodies... More about this image found in Identification of Cavin1-binding nanobodies. (A) Initial selections of nan...

Fig. 2. Nanobodies do not affect the in vitro intrinsic membrane-remodelling properties of Cavin1. (A) Liposome pelleting assay of the mouse Cavin1 (mC1)-HR1 domain in the presence of nanobodies. Multilamellar vesicles were generated from Folch I lipids. In the presence or absence of NbA12 or ... More about this image found in Nanobodies do not affect the in vitro intrinsic membrane-remodelling prop...

Fig. 3. NbB7 can be used as a molecular probe for Cavin1 in cells. (A) Co-immunoprecipitation of GFP-tagged nanobodies with Cavin1 and CAV1. HeLa cells expressing NbA12–GFP or NbB7–GFP were incubated with a GFP-binding nanobody coupled to NHS-activated Sepharose resin. Cavin1 and CAV1 were detec... More about this image found in NbB7 can be used as a molecular probe for Cavin1 in cells. (A) Co-immunopr...

Fig. 4. Molecular basis of nanobody NbB7 interaction with the mouse Cavin1 HR1 domain. (A) The crystal structure of trimeric mouse Cavin1 (mC1)-HR1 in complex with NbB7. The asymmetric unit contains one copy of the mC1-HR1 protein and one copy of NbB7, with the trimeric structure inferred from t... More about this image found in Molecular basis of nanobody NbB7 interaction with the mouse Cavin1 HR1 doma...

Fig. 5. Mutational analysis of the NbB7–Cavin1 interaction in cells. (A–D) Confocal images showing the localisation of transiently co-expressed NbB7–GFP nanobody with wild type (WT) or mutated mCherry–Cavin1 in HeLa Cavin1 knockout cells. Images for each channel were inverted into black and whit... More about this image found in Mutational analysis of the NbB7–Cavin1 interaction in cells. (A–D) Confoca...

Fig. 6. Altered dynamics in binding affinity between nanobodies and hydrogen-bonded pair mutants in Cavin1. (A) Heptad repeat sequence alignments of cavin proteins. The top panel represents the mouse Cavin1 (mC1) HR1 domain structure and the central residues are shown with sidechains. The bottom... More about this image found in Altered dynamics in binding affinity between nanobodies and hydrogen-bonded...

Fig. 7. Mutations in the Cavin1 HR1 domain inhibit recruitment of Cavin3 to caveolae. (A–D) Confocal images showing the localisation of transiently co-expressed mouse Cavin1–GFP mutants with mouse Cavin2–mCherry or mouse Cavin3–mCherry in PC3 cells. These cells lack endogenous cavins and only fo... More about this image found in Mutations in the Cavin1 HR1 domain inhibit recruitment of Cavin3 to caveola...

Structure of the yeast HOPS tethering complex. More about this image found in Structure of the yeast HOPS tethering complex.

Angelika Noegel in the seminar room at the Institute for Biochemistry I, University of Cologne, in 2008. More about this image found in Angelika Noegel in the seminar room at the Institute for Biochemistry I, Un...

Fig. 1. Substrate stiffness affects PSC migration and morphology. (A) Representative phase-contrast microscopy images of PSCs seeded on substrates with different stiffnesses, as indicated. Scale bars: 100 µm (B) Durotaxis polar plots depict individual PSC trajectories over 24 h (black lines) on ... More about this image found in Substrate stiffness affects PSC migration and morphology. (A) Representati...

Fig. 2. PSCs undergo durotaxis on linear stiffness gradient hydrogels. (A) Experimental setup of gradient gel construction. Glass-bottom dishes were coated with a UV polymerized hydrogel using a gradient photomask (grayscale form inside the dish). The resulting stiffness gradient of the gel was ... More about this image found in PSCs undergo durotaxis on linear stiffness gradient hydrogels. (A) Experim...

Fig. 3. Piezo1 is involved in PSC durotaxis. (A) Durotaxis polar plots of wild-type (Wt, left) and Piezo1 GFAP KO PSCs depict cell trajectories over 24 h (black lines). For each plot, the gradient orientation is towards the left side. The radii of the blue half circles on the right-hand and lef... More about this image found in Piezo1 is involved in PSC durotaxis. (A) Durotaxis polar plots of wild-typ...

Fig. 4. Multiple mechanosensitive channels are necessary for durotaxis. (A) Durotaxis polar plots depict cell trajectories over 24 h (black lines) of wild-type (Wt, top) and TRPC1 KO (TRPC1-KO, bottom) PSCs treated with the TRPV4 inhibitor 100 nM HC067047. For each plot, the gradient orientation... More about this image found in Multiple mechanosensitive channels are necessary for durotaxis. (A) Durota...

Fig. 5. The mathematical model supports the hypothesis of the dependence of durotaxis on ion channel function. (A) E denotes the elastic modulus of the substratum; it is larger for a stiffer material. The substratum's stiffness gradient is indicated in black. (B) The bell-shaped function q a... More about this image found in The mathematical model supports the hypothesis of the dependence of durotax...

Fig. 1. The HOPS Vps41 Δ527–640 mutant forms a hexameric complex and can be purified from yeast. (A) Density map generated from the atomic model of the yeast HOPS tethering complex (left; PDB: 7ZU0 and AlphaFold AF-P38959-F1) and a zoom-in of the predicted atomic model of Vps41 from wild-type... More about this image found in The HOPS Vps41 Δ527–640 mutant forms a hexameric complex and can be purifi...