... and store-operated Ca 2+ entry regulated by protein kinase C at PM–ER contact sites. ORP3 Phosphoinositides Protein kinase C STIM1 SOCE TIRF BRET National Institute of Child Health and Human Development 10.13039/100000071 National Institutes of Health HD000196-20...

... channels. TIRF microscopy shows that junctate enhances the number of fluorescent puncta on the plasma membrane. The size and distribution of these puncta are not affected by the addition of agonists that mobilize Ca 2+ from Ins(1,4,5) P 3 -sensitive stores. Puncta are associated with a significantly larger...

... for their experimental systems. Use TIRF-specific filters to avoid laser-light reflections and interference patterns, and broad BP or LP filters when possible. Similar to SD-CM, choose the appropriate camera on the basis of the need for high spatial resolution (scientific grade CCD) or speed (EM-CCD...

...Merel J. W. Adjobo-Hermans; Joachim Goedhart; Theodorus W. J. Gadella, Jr Basic knowledge as to the subcellular location and dynamics of PLCβ isozymes is scant. Here, we report on the subcellular location of GFP-PLCβ1a and the use of total internal reflection fluorescence (TIRF) microscopy...

... GAK Auxilin Clathrin Dynamin TIRF Clathrin-mediated endocytosis is one of the major mechanisms employed by cells for receptor internalization. Cargo-specific adaptors function to concentrate receptors in the clathrin-coated pits (CCPs). The CCPs then progressively invaginate, their necks...

[email protected] ) 5 5 2006 © The Company of Biologists Limited 2006 2006 Insulin Exocytosis SNARE TIRF Insulin secretion from pancreatic β-cells is an essential prerequisite to maintain appropriate blood glucose levels. Defects in this process can result in profound metabolic...

... Fluorescence (TIRF) microscopy to visualize the three-dimensional motions of secretory organelles near the plasma membrane in living endothelial cells. Weibel-Palade bodies (WPb), the large tubular storage organelles for von Willebrand factor, were labelled with Rab27a-GFP. By contrast, green fluorescent...

...G.A. Truskey; J.S. Burmeister; E. Grapa; W.M. Reichert ABSTRACT A simplified model of TIRF optics was used to quantitate the relative membrane/substratum separation distances from the spatial pattern of TIRF image brightness. Phase-contrast and total internal reflection fluorescence microscopy...

...W. M. Reichert; G. A. Truskey ABSTRACT Total Internal Reflection Fluorescence (TIRF) is a powerful technique for visualizing focal and close contacts between the cell and the surface. Practical application of TIRF has been hampered by the lack of straightforward methods to calculate separation...

... is relatively uniform beneath the entire cell . We briefly discuss the sensitivity of several cell types to TIRAF, the generation of ultrathin lamellae and the nature of the cell–glass gap. 21 08 1987 03 10 1987 © 1988 by Company of Biologists 1988 IRM TIRAF TIRF fluorescence...