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Keywords: TIRF
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Journal Articles
J Cell Sci (2020) 133 (6): jcs237388.
Published: 16 March 2020
... was used. Specific statistical analyses are given in the figure legends for each experiment. HEK293-AT1 cells were plated on IBIDI 8-well µSlides or on 96-well microplates at a density of 10 4 cells/well. One day later, cells were transfected with 20 µl (for TIRF) or 50 µl (for BRET) transfection...
Includes: Supplementary data
Journal Articles
J Cell Sci (2020) 133 (6): jcs237388.
Published: 16 March 2020
... and store-operated Ca 2+ entry regulated by protein kinase C at PM–ER contact sites. ORP3 Phosphoinositides Protein kinase C STIM1 SOCE TIRF BRET National Institute of Child Health and Human Development 10.13039/100000071 National Institutes of Health HD000196-20...
Includes: Supplementary data
Journal Articles
J Cell Sci (2010) 123 (23): 4170–4181.
Published: 1 December 2010
... channels. TIRF microscopy shows that junctate enhances the number of fluorescent puncta on the plasma membrane. The size and distribution of these puncta are not affected by the addition of agonists that mobilize Ca 2+ from Ins(1,4,5) P 3 -sensitive stores. Puncta are associated with a significantly larger...
Includes: Supplementary data
Journal Articles
J Cell Sci (2009) 122 (6): 753–767.
Published: 15 March 2009
.... Nagamatsu, S. and Ohara-Imaizumi, M. ( 2008 ). Imaging exocytosis of single insulin secretory granules with TIRF microscopy. Methods Mol. Biol. 440 , 259 -268. Nalbant, P., Hodgson, L., Kraynov, V., Toutchkine, A. and Hahn, K. M. ( 2004 ). Activation of endogenous Cdc42 visualized in living cells...
Includes: Multimedia, Supplementary data
Journal Articles
J Cell Sci (2008) 121 (22): 3770–3777.
Published: 15 November 2008
...Merel J. W. Adjobo-Hermans; Joachim Goedhart; Theodorus W. J. Gadella, Jr Basic knowledge as to the subcellular location and dynamics of PLCβ isozymes is scant. Here, we report on the subcellular location of GFP-PLCβ1a and the use of total internal reflection fluorescence (TIRF) microscopy...
Journal Articles
J Cell Sci (2006) 119 (17): 3502–3512.
Published: 1 September 2006
... but neither scission nor irreversible uncoating occur. Therefore, budding as well as synchronous recruitment of GAK might be required for irreversible clathrin uncoating. Although the flashing of GAK was clearly visible by TIRF microscopy, it was difficult to detect flashing of GFP-GAK on the plasma...
Includes: Multimedia, Supplementary data
Journal Articles
J Cell Sci (2006) 119 (14): 2912–2920.
Published: 15 July 2006
... Axiovert 200 inverted fluorescence microscope was modified to allow both EPI and TIRF illumination (Visitron Systems, Puchheim, Germany) as described previously ( Bezzi et al., 2004 ). For TIRF illumination, the expanded beam (488-568 nm argon-krypton multi-line laser, 20 mW; Laserphysics, Stockholm...
Journal Articles
J Cell Sci (2003) 116 (19): 3927–3938.
Published: 1 October 2003
... Fluorescence (TIRF) microscopy to visualize the three-dimensional motions of secretory organelles near the plasma membrane in living endothelial cells. Weibel-Palade bodies (WPb), the large tubular storage organelles for von Willebrand factor, were labelled with Rab27a-GFP. By contrast, green fluorescent...
Includes: Multimedia, Supplementary data
Journal Articles
Journal Articles
J Cell Sci (1990) 96 (2): 219–230.
Published: 1 June 1990
...W. M. Reichert; G. A. Truskey ABSTRACT Total Internal Reflection Fluorescence (TIRF) is a powerful technique for visualizing focal and close contacts between the cell and the surface. Practical application of TIRF has been hampered by the lack of straightforward methods to calculate separation...
Journal Articles