...Syara Fujii; Kazuo Kurokawa; Ryota Inaba; Naoki Hiramatsu; Tatsuya Tago; Yuri Nakamura; Akihiko Nakano; Takunori Satoh; Akiko K. Satoh ABSTRACT Historically, the trans-Golgi network (TGN) has been recognized as a sorting center of newly synthesized proteins, whereas the recycling endosome (RE...

...-Golgi network (TGN) to the bile canaliculus by basolateral sorting and endocytosis, and microtubule-mediated transcytosis through the subapical compartment. Trafficking ATP7B is not incorporated into lysosomes, and addition of Cu + does not cause relocalization of lysosomes and the appearance...

... Laboratory, RIKEN Advanced Science Institute, Saitama 351–0198, Japan 25 10 2011 © 2012. 2012 AP-1 Clathrin GGA Drosophila Notch TGN Endosomes Trafficking Intracellular transport of integral membrane proteins and secretion of soluble proteins both require formation...

... are mammalian SNARE partners, we propose that ENTH domain proteins at the TGN-endosome are cargo adaptors for these endosomal SNAREs. SNARE ENTH domain TGN Endosome Protein sorting The vesicle-mediated membrane transport is a multi-step process, consisting of vesicle formation (budding...

...Ton Hoai Thi Tran; Qi Zeng; Wanjin Hong VAMP4 is enriched in the trans-Golgi network (TGN) and functions in traffic from the early and recycling endosomes to the TGN, but its trafficking itinerary is unknown. Cells stably expressing TGN-enriched VAMP4 C-terminally-tagged with EGFP (VAMP4-EGFP...

...., Bujny, M. V., Peter, B. J., Oorschot, V. M. J., Rutherford, A. C., Mellor, H., Klumperman, J., McMahon, H. T. and Cullen, P. J. ( 2004 ). Sorting nexin-1 mediates tubular endosome-to-TGN transport through co-incidence sensing of high curvature membranes and 3-phosphoinositides. Curr. Biol. 14...

... contributed equally to this work © The Company of Biologists Limited 2004 2004 Synaptogenesis TGN Transport NCAM Neurons are highly polarized cells. A typical neuron in the central nervous system (CNS) consists of three distinct functional and organizational domains: a cell body (or soma...

...Ina Hinners; Sharon A. Tooze Clathrin-coated vesicles mediate transport between the trans-Golgi network(TGN) and endosomes. In recent years there has been tremendous progress in identifying factors involved in anterograde and retrograde transport steps. The well-characterised heterotetrameric...

...) of AE1-4 accumulated in intracellular membrane compartments that overlapped late endosomes and the trans-Golgi network (TGN), respectively. Internalization assays indicated that the patterns of localization observed for F c 1-37 and F c 38-63 resulted from the recycling of these chimeras from the cell...

... orthologues: roles in post-TGN vesicle formation and maintenance of TGN morphology . Biochem. Biophys. Acta. Mol. Cell Res . 1355 , 209 – 217 . 10.1016/S0167-4889(96)00146-2 Bleil , J. D. and Bretscher , M. S. ( 1982 ). Transferrin receptor and its recycling in HeLa-cells . EMBO J...

...Irene Wacker; Christoph Kaether; Andreas Krömer; Andrea Migala; Wolf Almers; Hans-Hermann Gerdes ABSTRACT Biosynthetic transport from the trans -Golgi network (TGN) to the plasma membrane (PM) is mediated by secretory vesicles. We analyzed secretory vesicle transport in real time using a GFP-tagged...

... compartment. We examined the distribution of membrane PAM in stably transfected AtT-20 cells and compared its localization to markers for the trans - Golgi network (TGN), endosomes, and lysosomes. At the light microscopic level, the distribution of membrane PAM does not overlap extensively with lysosomal...

...-Golgi vesicles, although ∼30% of rab6 is soluble. The distribution of the membrane-associated and the soluble forms is highly polarized. By confocal and EM immunocytochemistry it can be seen that most of rab6 is associated with the photoreceptor trans -Golgi cisternae, trans -Golgi network (TGN...