... used cells with or without an actin cap to dissect and interrogate the behaviors of the two actin layers in response to substrate topography and strain. It is known that the actin cap plays a crucial role in mechanosensing of environmental stiffness ( Kim et al., 2012 ) and mediates fast...

... medium. When present, actin microfilaments and focal adhesions were aligned along the substrate topographies. The width of the focal adhesions was determined by the width of the ridges in the underlying substrate. This work documents that biologic length-scale topographic features that model features...