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Keywords: Split-ubiquitin
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Journal Articles
J Cell Sci (2020) 133 (11): jcs240283.
Published: 11 June 2020
... feedback Split-ubiquitin Yeast Cdc42 A critical step during PBS assembly involves recruitment of the septins. The four septin units, Cdc11, Cdc12, Cdc3 and Cdc10, form octameric rods that polymerize into filaments and higher-order structures like rings and collars at the bud neck ( Bertin et al...
Includes: Supplementary data
Journal Articles
J Cell Sci (2020) 133 (3): jcs237982.
Published: 13 February 2020
... with the membrane. Highlighted Article: The vesicle-tethering and fusion factors Boi1 and Boi2 are shown to initiate the formation of actin filaments by recruiting the Bni1–Bud6 actin nucleation complex to sites of exocytosis. Polar growth Vesicular traffic Actin nucleation Cdc42 Split-Ubiquitin...
Includes: Supplementary data
Journal Articles
J Cell Sci (2005) 118 (4): 723–732.
Published: 15 February 2005
... for correspondence (e-mail: nils.johnsson@itg.fzk.de ) 24 11 2004 © The Company of Biologists Limited 2005 2005 Sec complex Split-ubiquitin Signal sequence Protein interaction Saccharomyces cerevisiae Protein translocation across the membrane of the endoplasmic reticulum (ER...
Journal Articles
J Cell Sci (2003) 116 (17): 3623–3634.
Published: 01 September 2003
...Jörg H. Eckert; Nils Johnsson The protein import machinery of the peroxisome consists of many proteins,collectively called the peroxins. By applying the split-ubiquitin technique we systematically tested the pair-wise interactions between the N ub -and C ub -labeled peroxins for the first time...
Journal Articles
J Cell Sci (2001) 114 (24): 4629–4635.
Published: 15 December 2001
.... A chimera consisting of the transmembrane domain of Mns1p fused to the catalytic domain of the Golgi α1,2-mannosyltransferase Kre2p was localized in the endoplasmic reticulum of Δpep4 cells and in the vacuoles of rer1/Δpep4 by indirect immunofluorescence. The split-ubiquitin system was used to determine...