...Simon Ullrich; Iliya Nadelson; Stefan Krebs; Helmut Blum; Heinrich Leonhardt; Irina Solovei ABSTRACT Transcription of the majority of eukaryotic genes is accompanied by splicing. The timing of splicing varies significantly between introns, transcripts, genes and species. Although quick co...

... addressing ‘where’ and ‘when’ gene transcription and RNA splicing occur within cells, emphasizing the dynamic spatial and temporal organization of the cell nucleus. Author for correspondence ( [email protected] ) Competing interests The authors declare no competing or financial interests...

... that SART3 associates via its N-terminal HAT domain with the 12S U2 snRNP. Further analysis showed that SART3 associates with the post-splicing complex containing U2 and U5 snRNP components. In addition, we observed an interaction between SART3 and the RNA helicase DHX15, which disassembles post-splicing...

...Pablo Montañés-Agudo; Simona Casini; Simona Aufiero; Auriane C. Ernault; Ingeborg van der Made; Yigal M. Pinto; Carol Ann Remme; Esther E. Creemers ABSTRACT Eukaryotic genomes contain a tiny subset of ‘minor class’ introns with unique sequence elements that require their own splicing machinery...

... understood. Here, we show that Hub1-mediated alternative splicing of HEH1 pre-mRNA, resulting in production of its shorter form Heh1-S, is critical for the integrity of the NE in Saccharomyces cerevisiae . ESCRT-III mutants lacking Hub1 or Heh1-S display severe growth defects and accumulate improperly...

... physically associates with the INQ substrate and splicing factor Hsh155, and regulates its assembly with partner proteins. Accordingly, cdc48 mutants have defects in splicing and show spontaneous distribution of Hsh155 to INQ aggregates, where it is stabilized. Overall, this study shows that Cdc48 regulates...

...Cristina Moreno-Castro; Silvia Prieto-Sánchez; Noemí Sánchez-Hernández; Cristina Hernández-Munain; Carlos Suñé ABSTRACT Cajal bodies are nuclear organelles involved in the nuclear phase of small nuclear ribonucleoprotein (snRNP) biogenesis. In this study, we identified the splicing factor TCERG1...

... matrix assembly. While the α integrin subunit determines extracellular ligand specificity, the β integrin chain binds to an acidic residue of the ligand, and cytoplasmic adapter protein families such as talins, kindlins and paxillin, to form mechanosensing cell matrix adhesions. Alternative splicing...

... as tools to manipulate splicing and for use as potential anti-cancer drugs. We investigated the effects of these inhibitors on mRNA transport and stability in human cells. Upon splicing inhibition, unspliced pre-mRNAs accumulated in the nucleus, particularly within enlarged nuclear speckles. However...

...Karen S. Frese; Benjamin Meder; Andreas Keller; Steffen Just; Jan Haas; Britta Vogel; Simon Fischer; Christina Backes; Mark Matzas; Doreen Köhler; Vladimir Benes; Hugo A. Katus; Wolfgang Rottbauer ABSTRACT Alternative splicing is one of the major mechanisms through which the proteomic...

... ( [email protected] ) © 2011. 2011 Circadian mRNA decay Translation Rhythmic mRNA stability Splicing The rotation of the earth creates a daily fluctuation of environmental cues. Organisms have evolved internal timing systems, called circadian clocks (see Box 1 for a glossary...

...-immunoprecipitated with human RNA polymerase II from mitotic cell extracts and identified U1 small nuclear RNA (snRNA) as a major species. To investigate a possible splicing-independent recruitment of U1 snRNA to transcription units, we established cell lines having integrated a reporter gene containing a functional...

... angiogenesis. Two families of VEGF isoforms are generated by alternate splice-site selection in the terminal exon. Proximal splice-site selection (PSS) in exon 8 results in pro-angiogenic VEGF xxx isoforms (xxx is the number of amino acids), whereas distal splice-site selection (DSS) results in anti-angiogenic...

...Kate A. Sergeant; Cyril F. Bourgeois; Caroline Dalgliesh; Julian P. Venables; James Stevenin; David J. Elliott The scaffold attachment factor SAFB1 and its recently discovered homologue SAFB2 might provide an important link between pre-mRNA splicing, intracellular signalling and transcription...

...Anabella Srebrow; Alberto R. Kornblihtt Alternative splicing is a crucial mechanism for generating protein diversity. Different splice variants of a given protein can display different and even antagonistic biological functions. Therefore, appropriate control of their synthesis is required...

... and comprises a histidine tail. A similar sequence is also responsible for the targeting of cyclin T1. Therefore the histidine-rich region represents a novel splicing speckle targeting signal. Moreover, overexpression of DYRK1A induces speckle disassembly. Such disassembly is DYRK1A activity specific, since...

... to Prp2, the large subunit of the essential splicing factor U2AF. In wat1 mutants, the cellular amount of α-tubulin is decreased to very low levels, which results in compromised microtubules and spindles, consequently leading to unequal chromosome separation. Further analysis shows that, in spite...

... and endonuclease. In yeast, Ire1p activates gene expression by promoting a non-conventional splicing event that converts the mRNA encoding the Hac1p transcription factor from an inefficiently translated inactive mRNA to an actively translated one. Hac1p binds to the promoters of genes encoding chaperones and other...

... of PP1’ (NIPP1), a major nuclear PP1-binding protein, shows a speckled nucleoplasmic distribution where it is colocalised with pre-mRNA splicing factors. One of these factors (Sm) is also shown to be complexed to NIPP1 in nuclear extracts. Immunodepletion of NIPP1 from nuclear extracts, or addition...

... of mouse genes encoding proteins that are confined to nuclear compartments by splicing endogeneous gene sequences to a promoterless βgeo reporter, using a gene trap approach. Mouse ES (embryonic stem) cell lines were identified that express βgeo fusions located within sub-nuclear compartments, including...