...Aude Pascal; Emmanuel Gallaud; Regis Giet; Christelle Benaud ABSTRACT Proper mitotic spindle orientation depends on the correct anchorage of astral microtubules to the cortex. It relies on the remodeling of the cell cortex, a process not fully understood. Annexin A2 (Anx2; also known as ANXA2...

...Haijing Guo; Jen-Hsuan Wei; Yijun Zhang; Joachim Seemann ABSTRACT Spindle orientation is important in multiple developmental processes as it determines cell fate and function. The orientation of the spindle depends on the assembly of a proper astral microtubule network. Here, we report...

...Riya Keshri; Ashwathi Rajeevan; Sachin Kotak ABSTRACT Proper orientation of the mitotic spindle is critical for accurate development and morphogenesis. In human cells, spindle orientation is regulated by the evolutionarily conserved protein NuMA, which interacts with dynein and enriches...

...Amit Sharma; Sunayana Dagar; Sivaram V. S. Mylavarapu ABSTRACT The molecular motor dynein is essential for mitotic spindle orientation, which defines the axis of cell division. The light intermediate chain subunits, LIC1 and LIC2, define biochemically and functionally distinct vertebrate dynein...

...Yvonne T. Kschonsak; Ingrid Hoffmann ABSTRACT Correct spindle orientation is achieved through signaling pathways that provide a molecular link between the cell cortex and spindle microtubules in an F-actin-dependent manner. A conserved cortical protein complex, composed of LGN (also known as GPSM2...

... division Spindle orientation Polyploidy Microcephaly Cancer The fascinating process of cell division is essential for growth, development and reproduction in many organisms. Cell division controls the correct segregation of genome and cytoplasm, including organelles, into daughter cells...

... orientation systems, ‘accessory pathways’ are often also required. In this work, we identified an accessory spindle orientation pathway in Drosophila that functions with Dynein during planar cell polarity, downstream of the Frizzled (Fz) effector Dishevelled (Dsh). Dsh contains a PDZ ligand and a Dynein...

... microtubule organization. Instead, we find that LIMK2 acts through TPPP in the regulation of astral microtubule organization, whereas both LIMK1 and LIMK2 affect centrosome focusing. Both phenotypes are tightly coupled to spindle orientation in the mitotic cells. Thus, our results reveal a new regulatory link...

...Jillian C. Pease; Jennifer S. Tirnauer Mitotic spindle orientation can influence tissue organization and vice versa. Cells orient their spindles by rotating them parallel or perpendicular to the cell – and hence the tissue – axis. Spindle orientation in turn controls the placement of daughter cells...

... and the kinesin Kip3p define redundant pathways that ensure proper spindle orientation. Furthermore, it has been shown that the Kip3p pathway components Kar9p and Bim1p (Yeb1p) form a complex that provides a molecular link between cortical polarity cues and spindle microtubules. Recently, other studies indicated...