...Michiel Boekhout; Rob Wolthuis Nek2 isoform A (Nek2A) is a presumed substrate of the anaphase‐promoting complex/cyclosome containing Cdc20 (APC/C Cdc20). Nek2A, like cyclin A, is degraded in mitosis while the spindle checkpoint is active. Cyclin A prevents spindle checkpoint proteins from binding...

...Scott C. Schuyler; Yueh-Fu Wu; Vivian Jen-Wei Kuan Summary Cancer cells are commonly aneuploid. The spindle checkpoint ensures accurate chromosome segregation by controlling cell cycle progression in response to aberrant microtubule–kinetochore attachment. Damage to the checkpoint, which...

... kinetochores and contributes to efficient microtubule attachment and proper chromosome alignment during mitosis. Cell Res. 18 , 268 -280. Davenport, J., Harris, L. D. and Goorha, R. (2006). Spindle checkpoint function requires Mad2-dependent Cdc20 binding to the Mad3 homology domain of BubR1. Exp...

... with the microtubule (Fig. 3C) (Wang et al., 2007). Dam1p is a major target of the spindle-checkpoint kinase Ipl1p (Cheeseman et al., 2002). Interestingly, a phosphomimetic mutant of the Ipl1p-phosphorylation sites in Dam1p (three out of four of which are at the Dam1p C-terminus) shows little effect on the binding...

... Spindle checkpoint Drugs that disrupt mitotic progression, which are commonly referred to as `anti-mitotics', are used extensively for the treatment of cancer. Currently, all such drugs that have been approved for clinical use target microtubules, with the taxanes and vinca alkaloids showing much...

... ; [email protected]) © The Company of Biologists Limited 2009 2009 Calcium Gene transcription Ins P 3 Nucleus Paclitaxel Ryanodine Signalling Spindle checkpoint Taxol Calcium (Ca 2+) is a key player in signal transduction. It modulates diverse cellular activities ranging from...

... Limited 2007 2007 Aneuploidy Spindle checkpoint Chromosome congression Mono-oriented chromosomes Among the cellular structures most responsible for the accurate distribution of chromosomes during cell division are the centromeres and their associated kinetochores. Centromeres...

...Rahul Pandey; Sebastian Heeger; Christian F. Lehner The dramatic chromosome instability in certain tumors might reflect a synergy of spindle checkpoint defects with hypoxic conditions. In Caenorhabditis elegans and Drosophila melanogaster , spindle checkpoint activation has been implicated...

... formation with normal timings; while both securin and cyclin B1 destruction, visualised by coupling to fluorescent protein tags, were complete by the time of polar body extrusion. Induction of a spindle checkpoint by overexpression of Mad2 or by spindle poisons blocked CamKII-induced resumption of meiosis...

...Christopher J. Morrow; Anthony Tighe; Victoria L. Johnson; Maria I.F. Scott; Claire Ditchfield; Stephen S. Taylor The spindle checkpoint maintains genome stability by inhibiting Cdc20-mediated activation of the anaphase promoting complex/cyclosome (APC/C) until all the chromosomes correctly align...

..., the protein appears to modulate microtubule function and might participate in regulating the spindle checkpoint. Survivin physically associates with Aurora B, a serine-threonine kinase involved in microtubule attachment to centromeres and regulation of chromosome segregation. Here we have examined...

... evoke the mitotic spindle checkpoint response. Whereas premature mitotic exit is dependent upon anaphase promoting complex function, premature spindle elongation is not. We conclude that nimU is constitutively required for orderly mitotic progression under normal growth conditions and also required...

...Leigh Campbell; Kevin G. Hardwick The spindle checkpoint delays the onset of anaphase if there are any defects in the interactions between spindle microtubules and kinetochores. This checkpoint has been reconstituted in vitro in Xenopus egg extracts, and here we use antibodies to Xenopus Bub3...

...Julie C. Canman; Nitin Sharma; Aaron Straight; Katie B. Shannon; Guowei Fang; E. D. Salmon Spindle checkpoint proteins, such as Mad2 and BubR1, and the motors dynein/dynactin and CENP-E usually leave kinetochores prior to anaphase onset by microtubule-dependent mechanisms. Likewise, `chromosome...

...Amy E. Ikui; Kanji Furuya; Mitsuhiro Yanagida; Tomohiro Matsumoto To ensure accurate chromosome segregation, the spindle checkpoint delays the onset of sister chromatid separation when the spindle is not attached to a kinetochore. Mad2, a component of the checkpoint, targets fission yeast Slp1...

... spermatocytes suggests that it is the application of tension across kinetochores which signals the ‘all clear’ for anaphase (Li and Nicklas, 1995). In budding yeast, inhibition of premature anaphase is dependent on the spindle checkpoint components Bub1, Bub3, Mad1, Mad2 and Mad3 (Hoyt et al., 1991 ; Li...

...R. Bruce Nicklas; Jennifer C. Waters; E. D. Salmon; Suzanne C. Ward The spindle checkpoint detects errors in kinetochore attachment to microtubules and delays anaphase if attachment is improper. The checkpoint is activated by attachment-sensitive components including Mad2 and certain phosphorylated...

... and also Lte1. Following spindle checkpoint activation, the cell cycle phosphorylation of Bfa1 and Lte1 is protracted and some species are accentuated. Thus, the Bub2-dependent pathway is active every cell cycle and the effect of spindle damage is simply to protract its normal function. Indeed, function...

... for correspondence (e-mail: [email protected]) 28 08 2000 17 10 2000 © 2000 by Company of Biologists 2000 Kinetochore Kinetochore microtubule Micromanipulation Spindle checkpoint Tension Tension plays important regulatory roles in mitosis. When a chromosome is properly attached...

... of a new spindle checkpoint gene, mphl (Mps1p-like pombe homolog), in the fission yeast Schizosaccharomyces pombe , that is required for checkpoint activation in response to spindle defects. mphl functions upstream of mad2 , a previously characterized component of the spindle checkpoint. Overexpression...