... permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. References Bokros , M. , Gravenmier , C. , Jin , F. , Richmond , D. and Wang , Y. ( 2016 ). Fin1-PP1 helps clear spindle assembly checkpoint...

...Yanze Jian; Lingyun Nie; Sikai Liu; Yueyue Jiang; Zhen Dou; Xing Liu; Xuebiao Yao; Chuanhai Fu ABSTRACT The outer kinetochore serves as a platform for the initiation of the spindle assembly checkpoint (SAC) and for mediating kinetochore–microtubule attachments. How the inner kinetochore subcomplex...

... and delays the progression of oocyte maturation. Importantly, inhibition of the spindle assembly checkpoint (SAC) bypassed the delay of oocyte maturation caused by DNA synthesis inhibition. Finally, we found that ∼20% of unperturbed oocytes experienced spontaneously arising damage during maturation...

... microtubule–kinetochore attachments to induce spindle assembly checkpoint (SAC; also known as the mitotic checkpoint)-activated mitotic arrest. SAC activation causes mitotic arrest by chronically activating Cdk1. One consequence of prolonged Cdk1 activation is cell death. However, the cytoplasmic signal(s...

...Banafsheh Etemad; Abel Vertesy; Timo E. F. Kuijt; Carlos Sacristan; Alexander van Oudenaarden; Geert J. P. L. Kops ABSTRACT The spindle assembly checkpoint (SAC) ensures proper chromosome segregation by monitoring kinetochore–microtubule interactions. SAC proteins are shed from kinetochores once...

... on the separation of spindle pole bodies (SPBs) and chromosome segregation. Our results show that failure to regulate the cMT lengths during G1-S phase prevents the separation of SPBs. This, in turn, affects chromosome capture and leads to the activation of spindle assembly checkpoint (SAC) and causes mitotic...

...Patrícia A. Simões; Ricardo Celestino; Ana X. Carvalho; Reto Gassmann ABSTRACT In mitosis, the molecular motor dynein is recruited to kinetochores by the Rod–Zw10–Zwilch complex (RZZ) and Spindly to control spindle assembly checkpoint (SAC) signaling and microtubule attachment. How the ubiquitous...

... by the spindle assembly checkpoint (SAC) when chromosomes have not attached to spindle microtubules. Unattached kinetochores catalyze the formation of a diffusible APC/C CDC20 inhibitor that comprises BUBR1 (also known as BUB1B), BUB3, MAD2 (also known as MAD2L1) and a second molecule of CDC20. Recruitment...

... of Biologists Ltd 2015 RZZ Polar body Spindle assembly checkpoint Mad1 Syncytial embryo Kinetochore Cell division is driven by oscillations in the activity of cyclin-dependent kinases (Cdks) and their associations with regulatory cyclin subunits. Mitotic entry is driven by high Cdk1–Cyclin-B...

.... These results imply that TRAIP regulates the spindle assembly checkpoint, MAD2 abundance at kinetochores and the accurate cellular distribution of chromosomes. The TRAIP ubiquitin ligase activity is functionally required for the spindle assembly checkpoint control. * Author for correspondence...

... contains multiple binding sites for the Bub proteins, with the Mps1-phosphorylated MELT repeats constituting individual functional docking sites for direct binding of Bub3. Surprisingly, chromosome congression and the spindle assembly checkpoint (SAC) are still functional when KNL1 is deleted of all...

...Valentina Rossio; Takeshi Michimoto; Takeshi Sasaki; Iwai Ohbayashi; Yoshiko Kikuchi; Satoshi Yoshida Summary Cdc55, a regulatory B-subunit of protein phosphatase 2A (PP2A) complex, is essential for the spindle assembly checkpoint (SAC) in budding yeast, but the regulation and molecular targets...

... of the spindle assembly checkpoint protein Mad2. This results in a strengthening of the spindle assembly checkpoint and renders cells more sensitive to the spindle poison paclitaxel. Constitutive Rb phosphorylation also causes a weakening of the p53-dependent tetraploidy checkpoint. Cells with overactive Cdk2...

...Stephanie Heinrich; Hanna Windecker; Nicole Hustedt; Silke Hauf Summary The spindle assembly checkpoint (SAC) blocks entry into anaphase until all chromosomes have stably attached to the mitotic spindle through their kinetochores. The checkpoint signal originates from unattached kinetochores, where...

...Pablo Lara-Gonzalez; Maria I. F. Scott; Maria Diez; Onur Sen; Stephen S. Taylor The spindle assembly checkpoint (SAC) is a signalling network that delays anaphase onset until all the chromosomes are attached to the mitotic spindle through their kinetochores. The downstream target of the spindle...

...Frederick G. Westhorpe; Anthony Tighe; Pablo Lara-Gonzalez; Stephen S. Taylor Accurate chromosome segregation requires the spindle assembly checkpoint to be active at the onset of mitosis, before being silenced following chromosome alignment. p31 comet is a checkpoint antagonist in that its...

.... , Musacchio A. ( 2009 ). Sister chromatid tension and the spindle assembly checkpoint . Curr. Opin. Cell Biol. 21 , 785 - 795 . Nishiyama T. , Ladurner R. , Schmitz J. , Kreidl E. , Schleiffer A. , Bhaskara V. , Bando M. , Shirahige K. , Hyman...

...Doruk Emre; Régine Terracol; Anaïs Poncet; Zohra Rahmani; Roger E. Karess Unattached kinetochores generate an anaphase inhibitor, through the spindle assembly checkpoint (SAC), that allows cells more time to establish proper kinetochore–microtubule (K–MT) linkages and thus avoid aneuploidy. Mad1...

... a new role for NudE. In mutant neuroblasts, chromosomes fail to congress to a tight metaphase plate, and cell division arrests because of spindle assembly checkpoint (SAC) activation. The targeting of NudE to mitotic kinetochores requires the dynein-interacting protein Lis1, and surprisingly Cenp-meta...

...Jun Zhou; Joyce Yao; Harish C. Joshi Faithful transmission of chromosomes during mitosis is ensured by the spindle assembly checkpoint. This molecular safeguard examines whether prerequisites for chromosome segregation have been satisfied and thereby determines whether to execute or to delay...