... spermatocytes expressing fluorescently tagged centriolar proteins. We demonstrated that an essential microtubule polymerisation factor, Orbit (the Drosophila CLASP orthologue, encoded by chb ), accumulated at the distal end of centrioles and was required for the elongation. Conversely, a microtubule-severing...

... regarding the physical division of primary spermatocytes during meiosis I. Here, we investigated this process using high-resolution time-lapse confocal microscopy and examined the spatiotemporal regulation of contractile ring assembly in C. elegans primary spermatocytes. We found that centralspindlin...

... kinases (PLKs) in mouse spermatocytes, both in vivo and during prophase exit induced ex vivo by the phosphatase inhibitor okadaic acid. All four PLKs are expressed during the first wave of spermatogenesis. Only PLK1 (not PLK2–4) localizes to the SC during the G2/MI transition. The SC central element...

...Madalina Tarsounas; Ronald E. Pearlman; Peter B. Moens ABSTRACT The phosphatase inhibitor okadaic acid accelerates meiotic events in rodent germ cells in culture. Isolated pachytene spermatocytes treated with okadaic acid proceed to a metaphase I arrest in a few hours as opposed to the similar...

...Nadine Gérard; Anne Corlu; Bernard Kneip; Henri Kercret; Maryvonne Rissel; Christiane Guguen-Guillouzo; Bernard Jégou ABSTRACT We have identified a liver-regulating protein involved in cell contact-mediated regulation of Sertoli cell function by primary spermatocytes in rat testis. Liver-regulating...

...Martin Bastmeyer; David G. Russell ABSTRACT Pales ferruginea spermatocytes, because of their high proportion of dividing cells, offer many advantages in the study of the meiotic spindle. In the present study we have combined ultrastructural and immunofluorescence techniques with biochemical...