...Xin Xu; Lifen Qiu; Maoxiang Zhang; Guangyu Wu ABSTRACT G protein-coupled receptors (GPCRs) constitute the largest superfamily of cell surface signaling proteins that share a common structural topology. When compared with agonist-induced internalization, how GPCRs are sorted and delivered...

...Matthew N. J. Seaman; Aamir S. Mukadam; Sophia Y. Breusegem ABSTRACT The retromer complex is a vital component of the endosomal protein sorting machinery necessary for sorting into both the endosome-to-Golgi retrieval pathway and also the endosome-to-cell-surface recycling pathway. Retromer...

... Secretory pathway Sorting TM9 protein Transmembrane domain Localization of membrane receptors at the surface of eukaryotic cells is often essential for them to perform their specific functions, such as cellular adhesion (e.g. integrins), nutrients capture (e.g. transferrin receptor) or signal...

... with CIN85, resulting in reduced EGFR ubiquitylation. Taken together, these data provide a mechanistic explanation of how SEPT9, though acting exclusively at the plasma membrane, impairs the sorting of EGFRs into the degradative pathway. * Author for correspondence ( [email protected]...

.... , Faundez   V. ( 2008 ). Phosphatidylinositol-4-kinase type II alpha contains an AP-3-sorting motif and a kinase domain that are both required for endosome traffic.   Mol. Biol. Cell   19 , 1415 – 1426 . 10.1091/mbc.E07-12-1239 Ellena   J. F. , Liang   B. , Wiktor   M. , Stein...

... mechanisms of sorting. In this Commentary, we discuss the recent advances in the field of GPI-AP sorting focusing on the mechanisms operating at the level of the exit from the ER and from the trans-Golgi network (TGN), which take place, respectively, in yeast and in polarized mammalian cells. By considering...

... hormone hyposecretion, supporting essential PREPL functions in AP-1-dependent secretory pathways. * Author for correspondence ( [email protected] ) 2 1 2013 © 2013. Published by The Company of Biologists Ltd 2013 Adaptin Clathrin Prolyl-oligopeptidase Sorting Trans-Golgi network...

...Matthew N. J. Seaman Summary The retromer complex is a vital element of the endosomal protein sorting machinery that is conserved across all eukaryotes. Retromer is most closely associated with the endosome-to-Golgi retrieval pathway and is necessary to maintain an active pool of hydrolase...

...Valentina Mercanti; Anna Marchetti; Emmanuelle Lelong; Franck Perez; Lelio Orci; Pierre Cosson Efficient sorting of proteins is essential to allow transport between intracellular compartments while maintaining their specific composition. During endocytosis, membrane proteins can be concentrated...

...Simona Paladino; Stephanie Lebreton; Simona Tivodar; Vincenza Campana; Rosaria Tempre; Chiara Zurzolo To understand the mechanism involved in the apical sorting of glycosylphosphatidylinositol (GPI)-anchored proteins (GPI-APs) we fused to the C-terminus of GFP the GPI-anchor-attachment signal...

...Anna Dukhovny; Andreas Papadopulos; Koret Hirschberg The sorting and concentration of cargo proteins within ER exit sites (ERESs) is a fundamental function of the secretory machinery. The mechanism by which peripheral coat complexes and their small GTPase effectors mediate this function with export...

...Elisabetta Zuccato; Emma J. Blott; Oliver Holt; Sara Sigismund; Michael Shaw; Giovanna Bossi; Gillian M. Griffiths Fas ligand (FasL), a potent mediator of apoptosis expressed by CTL and NK cells, is sorted into the inner vesicles of secretory lysosomes for release via exosome-like vesicles...

... generated missense and deletion mutants of full-length OA1. Using this approach, we identified two separate sorting signals that are both necessary and sufficient for intracellular retention, as well as lysosomal and melanosomal localization, in melanocytic and non-melanocytic cells. These sorting signals...

.... paxB – strains are less adhesive to the substrate, they can aggregate but multicellular development from the mound stage onwards is severely impeded. paxB – strains are defective in proper cell type proportioning, cell sorting, slug migration and form-defective fruiting bodies. Mutation of a conserved...

... on the sorting and recycling of membrane proteins. In this study we show that the type I membrane protein Tb MBAP1, an L-(+)-tartrate-sensitive acid phosphatase, is present in all endosomal membranes but is virtually absent from the lysosome membrane (where this type of protein is mainly found in other organisms...

...-neuronal secretory cells. * Author for correspondence (e-mail: [email protected] ) 6 1 2005 © The Company of Biologists Limited 2005 2005 Sorting Glycosylation Synaptotagmin RBL-2H3 Synaptotagmins comprise a family of structurally related proteins that are highly...

... release, the present study establishes that a CgA-GFP fusion protein expressed in neuroendocrine PC12 cells is trafficked to the dense core secretory granule and thereby sorted to the regulated pathway for exocytosis. We show that information necessary for such trafficking is contained within the N...

... in the localisation of procollagen to transport complexes that now also contain ERGIC-53 and are inhibited in their transport to the Golgi complex. These data reveal the existence of an early COPI-dependent,pre-Golgi cargo sorting step in mammalian cells. (e-mail: [email protected] pepperko@embl...

... significant sequence homologies with several melanosomal proteins. We have studied its intracellular traffic in both pigmented and non-pigmented cells. We report that a di-leucine-based sorting signal (ExxPLL) present in the cytoplasmic domain of QNR-71 is necessary and sufficient for its proper targeting...

... suggest that cholesterol is required for ECV/MVB reorganization that drives the sorting/transport of materials destined for the Golgi out of the pathways towards lysosomes. * Author for correspondence (e-mail: [email protected] ) 13 02 2001 © 2001 by Company of Biologists 2001...