...Stella Alimperti; Hui You; Teresa George; Sandeep K. Agarwal; Stelios T. Andreadis ABSTRACT Although soluble factors, such as transforming growth factor β1 (TGF-β1), induce mesenchymal stem cell (MSC) differentiation towards the smooth muscle cell (SMC) lineage, the role of adherens junctions...

... expression of α-SMA. These results suggest that SPC-stimulated secretion of TGF-β1 plays a crucial role in SPC-induced smooth muscle cell (SMC) differentiation through a Smad2-dependent pathway. Both SPC and TGF-β increased the expression levels of serum-response factor (SRF) and myocardin, transcription...

... of endogenous UPA in mediating the mitogenic effects of platelet-derived growth factor (PDGF) and basic fibroblast growth factor(bFGF) on human vascular smooth muscle cells (SMC). Growth-arrested SMC constitutively expressed UPA, but UPA expression and secretion increased several times upon stimulation...

...Laura A. Maile; Jane Badley-Clarke; David R. Clemmons ABSTRACT The disintegrin echistatin inhibits ligand occupancy of the αVβ3 integrin and reduces Insulin-like growth factor I (IGF-I) stimulated migration, DNA synthesis, and receptor autophosphorylation in smooth muscle cells. This suggests...

...Enrique A. Mesri; Minoru Ono; Robert J. Kreitman; Michael Klagsbrun; Ira Pastan SUMMARY Heparin-binding EGF-like growth factor (HB-EGF) is a smooth muscle cell mitogen composed of both EGF receptor and heparin-binding domains. To better understand the function of its domains, intact HB-EGF or its...

... lesions. Such heterogeneity appears to result from the presence of different smooth muscle cell phenotypes (contractile and synthetic) in these lesions. In the present study, we show that bovine aortic endothelial cells, which are characterised by the presence of Factor VIII-related antigen (FVIII...

... into RPMI 1640 pH 8.2 over 24 hours at 4°C with three changes of buffer and the dialysates were filtered through a 0.22 μm filter. * Author for correspondence 21 04 1992 03 07 1992 © 1992 by Company of Biologists 1992 smooth muscle cells conditioned medium LDL receptor...

... by sub-culturing has not been examined. Strategies for reducing the proliferation of these non-epithelial cells are also described. * Author for correspondence 17 06 1991 25 09 1991 © 1992 by Company of Biologists 1992 intestine epithelial in vitro smooth muscle cells...

...Lena Palmberg; Jan Åke Lindgren; Johan Thyberg; Hans-Erik Claesson ABSTRACT The induction of DNA replication in rat aortic smooth muscle cells (SMCs) by leukotrienes (LTs) was studied in order to elucidate the mechanisms of action in further detail. The effect of LTB 4 was blocked...

...Lena Palmberg; Hans-Erik Claesson; Johan Thyberg ABSTRACT During the first few days in primary culture arterial smooth muscle cells (SMCs) go through a transition from a contractile to a synthetic phenotype. Morphologically, this process includes loss of myofilaments and formation of an extensive...

...Lena Palmberg; Hans-Erik Claesson; Johan Thyberg ABSTRACT The effects of leukotrienes on initiation of DNA synthesis in growth-arrested arterial smooth muscle cells cultivated in a defined, serum-free medium were studied. The results showed that LTB 4 , LTC 4 and LTD 4 were all stimulatory...