...Jiaoyue Long; Guoning Liao; Yinna Wang; Dale D. Tang ABSTRACT The tyrosine kinase c-Abl participates in the regulation of various cellular functions including cell proliferation, adhesion, migration, smooth muscle contraction and cancer progression. However, knowledge regarding transcriptional...

... Ca 2+ release often begins at a single site, then regeneratively propagates through the cell as a Ca 2+ wave. The Ca 2+ wave consistently begins at the same site on successive activations. Here, we address the mechanisms that determine the Ca 2+ wave initiation site in intestinal smooth muscle cells...

...D. MacMillan; C. Kennedy; J. G. McCarron Summary Adenosine 5′-triphosphate (ATP) mediates a variety of biological functions following nerve-evoked release, via activation of either G-protein-coupled P2Y- or ligand-gated P2X receptors. In smooth muscle, ATP, acting via P2Y receptors (P2YR), may act...

...Brant E. Isakson Inositol (1,4,5)-trisphosphate [Ins(1,4,5) P 3 ] originating in the vascular smooth-muscle cells (VSMCs) has been shown to modulate the Ca 2+ stores in endothelial cells (ECs). However, the reverse is not found, suggesting that Ins(1,4,5) P 3 movement might be unidirectional across...

...John G. McCarron; Susan Chalmers; Thomas C. Muir Smooth muscle responds to activation of the inositol (1,4,5)-trisphosphate receptor [Ins(1,4,5) P 3 R] with a graded concentration-dependent (`quantal') Ca 2+ release from the sarcoplasmic reticulum (SR) store. Graded release seems incompatible both...

... m was examined during Ca 2+ oscillations in smooth muscle cells. In single, voltage-clamped smooth muscle cells, inhibition of mitochondrial Ca 2+ accumulation inhibited inositol (1,4,5)-trisphosphate [Ins(1,4,5) P 3 ]-evoked Ca 2+ release and prolonged the time required for restoration of [Ca 2...

...Nicolas Fritz; Jean-Luc Morel; Loice H. Jeyakumar; Sidney Fleischer; Paul D. Allen; Jean Mironneau; Nathalie Macrez Ryanodine receptor subtype 1 (RyR1) has been primarily characterized in skeletal muscle but several studies have revealed its expression in smooth muscle. Here, we used Ryr1 -null...

... that potentiates Ca 2+ release from the IP 3 R in smooth muscle. The presence of FKBP12 was confirmed in colonic myocytes and co-immunoprecipitated with the IP 3 R. In aortic smooth muscle, however, although present, FKBP12 did not co-immunoprecipitate with IP 3 R. In voltage-clamped single colonic myocytes...

... the hypothesis that MARCKS is a regulator of the spatial distribution of CaM within the cytoplasm of differentiated smooth-muscle cells. In overlay assays with portal-vein homogenates, CaM binds predominantly to the MARCKS-containing band. MARCKS is abundant in portal-vein smooth muscle (∼16 μM) in comparison...

... Acetylcholine Smooth muscle Ca 2+ signalling is the most `universal and versatile' signal transduction mechanism, mediating the intracellular effects of environmental signals into specific cellular responses ( Berridge et al., 2000 ). The specificity of the Ca 2+ signal depends on Ca 2+ channel...

...Gerald Burgstaller; Mario Gimona Activation of conventional protein kinase C by phorbol ester triggers the Src-dependent remodelling of the actin cytoskeleton and the formation of podosomes in vascular smooth muscle cells. Rearrangement of actin cytoskeleton in response to phorbol-12,13-dibutyrate...

...Irina Kaverina; Theresia E. B. Stradal; Mario Gimona Phorbol ester triggers the conversion of focal adhesions into podosomes in A7r5 smooth muscle cells. Here we followed the dynamics of podosome formation using dual fluorescence live video and confocal microscopy, as well as interference...

...KAZUHIRO KOHAMA; YUAN LIN; HIROMI TAKANO-OHMURO; RYOKI ISHIKAWA Summary A protein was purified from chicken gizzard smooth muscle. It bound ATP and actin. Actin activated the Mg 2+ -ATPase activity of this protein. The Ca 2+ -ATPase activity was lower than K + -EDTA ATPase activity. Thus...