... to destinations; however, the sorting mechanism remains largely unknown. Here, we show that ARF6 plays a general role in targeting palmitoylated proteins from the Golgi to the plasma membrane (PM). Through shRNA screening, we identified ARF6 as the key small GTPase in targeting CD36, a palmitoylated protein, from...

...Yuki Hatoyama; Yuta Homma; Shu Hiragi; Mitsunori Fukuda ABSTRACT Two small GTPases, Rab1 and Rab5, are key membrane trafficking regulators that are conserved in all eukaryotes. They have recently been found to be essential for cell survival and/or growth in cultured mammalian cells, thereby...

... to the F2 domain for its recruitment to adhesion sites to trigger integrin activation. Integrin Small GTPase Rap1 Talin RIAM NMR National Institutes of Health 10.13039/100000002 NIH P01HL073311 Max-Planck-Gesellschaft Deutsche Forschungsgemeinschaft 10.13039...

... binds tubulin to maintain uniform distribution of proteins along the ciliary membrane. Primary cilia Arl13b Ciliopathies Joubert syndrome Ciliary membrane Small GTPase Tubulin Axoneme Icahn School of Medicine at Mount Sinai 10.13039/100007277 National Institutes...

...Miho Oka; Keisuke Hashimoto; Yoshifumi Yamaguchi; Shin-ichiro Saitoh; Yuki Sugiura; Yuji Motoi; Kurara Honda; Yorifumi Kikko; Shinya Ohata; Makoto Suematsu; Masayuki Miura; Kensuke Miyake; Toshiaki Katada; Kenji Kontani ABSTRACT The small GTPase Arl8b localizes primarily to lysosomes...

...Hironori Inaba; Koji Yoda; Hiroyuki Adachi ABSTRACT Macropinocytosis involves the uptake of large volumes of fluid, which is regulated by various small GTPases. The Dictyostelium discoideum protein GflB is a guanine nucleotide exchange factor (GEF) of Rap1, and is involved in chemotaxis. Here, we...

...Hirohiko Iwasaki; Tomohiro Yorimitsu; Ken Sato ABSTRACT The COPII coat and the small GTPase Sar1 mediate protein export from the endoplasmic reticulum (ER) via specialized domains known as the ER exit sites. The peripheral ER protein Sec16 has been proposed to organize ER exit sites. However...

..., such as granule recruitment to the fusion site, whereas Rab2a functions in the early steps, such as granule biogenesis and maturation. Here, we demonstrate that these two small GTPases simultaneously bind to Noc2 (also known as RPH3AL) in a GTP-dependent manner, although Rab2a binds only after Rab27a has bound...

... PI3K and induces endocytosis through promoting signaling crosstalk between Arf6 and Rab27a pathways. Endocytosis Small GTPase Insulin Membrane trafficking Diabetes Pancreatic β-cells KAKENHI 19790636 21790876 23791039 26461342 Takeda Science Foundation...

... Highlighted Article: A combination of a Rab7 sensor and confocal FRET imaging techniques revealed that the Mon1–Ccz1 complex activates Rab7 only on late endosome and dissociates from lysosome in mammalian cells. Small GTPase FRET Endolysosomal pathway GEF Endocytosis and endolysosomal...

... subfamily of small GTPases. The guanine-nucleotide-exchange factor (GEF) P-Rex1 is engaged downstream of GPCRs and phosphoinositide 3-kinase (PI3K) in many cell types, and promotes tumorigenic signalling and metastasis in breast cancer and melanoma, respectively. Although P-Rex1-dependent functions have...

... of the actin-dependent melanosome transport and the mechanism of the microtubule-dependent retrograde melanosome transport in mammalian skin melanocytes have already been determined, almost nothing is known about the mechanism of the microtubule-dependent anterograde melanosome transport. Small GTPase Rab...

...Ivan de Curtis; Jacopo Meldolesi Summary Small GTPases are known to regulate hundreds of cell functions. In particular, Rho family GTPases are master regulators of the cytoskeleton. By regulating actin nucleation complexes, Rho GTPases control changes in cell shape, including the extension...

... in vertebrates. * Authors for correspondence ( [email protected] ; [email protected] ) © 2011. 2011 Cellular immunity Haemocyte Integrin Phagocytosis Parasitisation Small GTPase Ever since Boman et al. ( Boman et al., 1972 ) published their seminal paper showing...

... and cell migration. Crk-dependent signaling is crucial during the early stages of PDGFβR activation, whereas its termination by Abl family tyrosine kinases is important for turnover of focal adhesions and progression of dorsal-membrane ruffles. CrkII and CrkL preferentially activate the small GTPase Rac1...

... separation resulting in the formation of large clusters of cells that are connected by their primary septum ( Sandrock et al., 2006 ; Weinzierl et al., 2002 ). Cell division Ustilago maydis Germinal centre kinase Chemical genetics Small GTPase © The Company of Biologists Limited 2008...

...Takuro Okai; Yasuhiro Araki; Minoru Tada; Toshiyuki Tateno; Kenji Kontani; Toshiaki Katada The small GTPase superfamily, which includes the Ras, Rho/Rac, Rab, Arf and Ran subfamilies, serves as a signal transducer to regulate cell proliferation and differentiation, actin cytoskeleton, membrane...

[email protected] ) 16 6 2004 © The Company of Biologists Limited 2004 2004 Small GTPase GEF Tiam1 Actin cytoskeleton Cell adhesion Melanoma Migration Invasion The ability of cells to adopt a variety of shapes, and to carry out coordinated and directed movements...

.../HEF1 association domain also shows sequence similarity with guanine nucleotide exchange factors for Ras family small GTPases. In this study, we found significant activation of Rap1 in Chat-overexpressing cells. Myr-Chat, a membrane-targeted form of Chat,activated Rap1 more efficiently. Interestingly...

... morphogenesis. We previously implicated the small GTPase Drac1 in the assembly of an actomyosin contractile apparatus, contributing to cell shape change in the epidermis during dorsal closure. We now present evidence that Drac1 and Crumbs, a determinant of epithelial polarity, are involved in setting up...