...: The corepressor TLE4 maintains muscle stem cell quiescence and its downregulation is crucial for Myf5 activation and differentiation. Skeletal muscle Satellite cells Regeneration Quiescence Pax7 TLE4 Myf5 Mouse Science and Engineering Research Board http://dx.doi.org/10.13039/501100001843...

.... Welch's correction was applied for values of reticular calsequestrin-1-GFP and polymeric calsequestrin-1-GFP. FRET analyses were performed on primary skeletal muscle cells that were fixed 4, 8 or 12 days after differentiation had been induced. Fixation was obtained with 3% paraformaldehyde and 2...

...Helena Pinheiro; Mafalda Ramos Pimentel; Catarina Sequeira; Luís Manuel Oliveira; Anna Pezzarossa; William Roman; Edgar R. Gomes ABSTRACT Skeletal muscle myofibers are large and elongated cells with multiple and evenly distributed nuclei. Nuclear distribution suggests that each nucleus influences...

...-206 deletion shifts slow muscles to a faster profile and results in cardiac dysfunction specifically in male mice. miR-206 miRNA Skeletal muscle Heart Sexual dimorphism Skeletal muscle is a highly organized contractile tissue that comprises 40% of human body mass ( Janssen et al...

...Osvaldo Contreras; Meilyn Cruz-Soca; Marine Theret; Hesham Soliman; Lin Wei Tung; Elena Groppa; Fabio M. Rossi; Enrique Brandan ABSTRACT Fibro–adipogenic progenitors (FAPs) are tissue-resident mesenchymal stromal cells (MSCs) required for proper skeletal muscle development, regeneration...

.... Zammit; Henning Wackerhage ABSTRACT VGLL proteins are transcriptional co-factors that bind TEAD family transcription factors to regulate events ranging from wing development in fly, to muscle fibre composition and immune function in mice. Here, we characterise Vgll3 in skeletal muscle. We found...

... aging, immunodeficiency, cancer predisposition, growth retardation and motor defects, but not cerebellar neurodegeneration and ataxia. We explored whether Atm loss is responsible for skeletal muscle defects by investigating myofiber morphology, oxidative/glycolytic activity, myocyte ultrastructural...

... of elongated mitochondrial constrictions, providing novel structural evidence for mitochondrial dynamics stages in mouse fast-twitch muscle fibers. Fatigue Fission Mitochondria Skeletal muscle Mitochondria, the cells' permanent guests, are very dynamic and extremely sensitive...

...Paul Knopp; Yvonne D. Krom; Christopher R. S. Banerji; Maryna Panamarova; Louise A. Moyle; Bianca den Hamer; Silvère M. van der Maarel; Peter S. Zammit ABSTRACT Skeletal muscle wasting in facioscapulohumeral muscular dystrophy (FSHD) results in substantial morbidity. On a disease-permissive...

... Summary: Collagen VI is a remarkable component of the extracellular matrix, and studies have highlighted a crucial role for this protein in a wide range of tissues under physiological and pathological conditions. Collagen Extracellular matrix Skeletal muscle Collagen VI (ColVI...

... in myofibrillogenesis in chick skeletal muscle: evidence that thin filaments rearrange to form striated myofibrils.   J. Cell Sci.   112 , 1111 – 1123 . Almenar-Queralt   A. , Lee   A. , Conley   C. A. , Ribas de Pouplana   L. , Fowler   V. M. ( 1999b ). Identification of a novel tropomodulin...

...Laura Collard; Gaëlle Herledan; Alessandra Pincini; Aline Guerci; Voahangy Randrianarison-Huetz; Athanassia Sotiropoulos ABSTRACT Skeletal muscle atrophy is a debilitating process that is associated with a wide variety of conditions including inactivity, disease and aging. Here, we demonstrate...

... unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. Proteasome Autophagy Skeletal muscle Muscle atrophy The ubiquitin–proteasome and autophagy–lysosome pathways are the two major routes for protein and organelle clearance...

...Yu Xin Wang; Nicolas A. Dumont; Michael A. Rudnicki ABSTRACT Muscle stem cells facilitate the long-term regenerative capacity of skeletal muscle. This self-renewing population of satellite cells has only recently been defined through genetic and transplantation experiments. Although muscle stem...

... remain. Therefore, to better define the functional roles of synemin as a cytoskeletal protein and/or as an AKAP in skeletal muscle, we generated synemin knockout ( Synm − / − ) mice. Synm − / − mice were viable and fertile, enabling the investigation of molecular and phenotypic changes in adult...

... to insulin-stimulated glucose transport are unknown. We tested the impact of H 2 O 2 on insulin-dependent glucose transport and GLUT4 translocation in skeletal muscle cells. H 2 O 2 increased the translocation of GLUT4 with an exofacial Myc-epitope tag between the first and second transmembrane domains...

... previous studies have demonstrated that Sharp-1 is a negative regulator of skeletal myogenesis and it blocks differentiation of muscle precursor cells by modulating the activity of MyoD. In order to understand its role in pre- and post-natal myogenesis, we assessed skeletal muscle development and freeze...

...Chibeza C. Agley; Anthea M. Rowlerson; Cristiana P. Velloso; Norman R. Lazarus; Stephen D. R. Harridge Summary We characterised the adherent cell types isolated from human skeletal muscle by enzymatic digestion, and demonstrated that even at 72 hours after isolation these cultures consisted...

... of dysfunctional organelles and protein aggregates, whereas the ubiquitin-proteasome is important for the quality control of proteins. Post-mitotic tissues, such as skeletal muscle, are particularly susceptible to aged or dysfunctional organelles and aggregation-prone proteins. Therefore, these degradation systems...

...) generation and characterization of a novel knockin mouse model with a premature stop codon in the nebulin gene, eliminating its C-terminal SH3 domain (NebΔSH3 mouse). Surprisingly, detailed analyses of NebΔSH3 mice revealed no structural or histological skeletal muscle abnormalities and no changes in gene...