... between the resolutions of CLSM and FRET experiments (∼300 nm and 1-10 nm, respectively). In addition, NSOM allows single-molecule detection even in densely packed areas. As such, the use of NSOM combined with single-molecule detection sensitivity has provided new insights into the organization...

..., reduces background fluorescence from the cytoplasm to the extent that single-molecule detection sensitivity becomes within reach. NSOM allows detection of individual fluorescent proteins as part of multimolecular complexes on the surface of fixed cells, and similar results should be achievable under...