... Motor protein Intracellular transport Single molecule Live imaging National Science Foundation 10.13039/100000001 MCB-1121287 CMMI-1548571 Kinesins execute various cellular functions either by powering directional transport of cargo along microtubules or by regulating...

..., containing 1.0 µM of Sfp synthase (New England Biolabs), 10 mM MgCl 2 and variable amounts of coenzyme A derivative depending on the experiment. For the labelling with S-Qdots for single-molecule experiments, 5 µM biotin-CoA was used, then cells were washed twice in PBS before incubation for 2 minutes...

... the powerful set of advanced AFM techniques (e.g. live-cell imaging and single-molecule manipulation) can be integrated with the modern tools of molecular genetics (i.e. protein design) to study the localization and molecular elasticity of individual mechanosensors on the surface of living cells. Although we...

... implementation of single molecule tools constituting fluorescence correlation spectroscopy (FCS), pulse interleave excitation-based Förster resonance energy transfer (PIE-FRET) and fluorescence lifetime imaging-based FRET (FLIM-FRET) to elucidate the composition of single nucleosomes containing histone variant...

... quantitative force actuation and force measurement that is sufficiently sensitive to characterize the interaction of single molecules. Therefore, not only overall cell adhesion but also the properties of single adhesion-receptor–ligand interactions can be studied. Here we describe current implementations...