... the cortical granules for their timely exocytosis is largely unknown. In Caenorhabditis elegans , the recruitment of separase, a downstream effector of the fertilization signal, to the cortical granules is essential for exocytosis because separase is required for membrane fusion. However, the molecule...

... Separase PLCε is a dual function protein, incorporating PLC and GEF enzyme activities. In addition, it has a Ras-association domain (RA2) that mediates interaction with several GTPases from Ras family (H-, K-, N-Ras, Rap1 and Rap2) ( Fig. 1A ) ( Bunney and Katan, 2006 ; Smrcka et al., 2012...

... that premature disengagement results from untimely activation of the anaphase-promoting complex (APC/C), leading to securin degradation and release of active separase. Although APC/C activation during G2 arrest is dependent on polo-like kinase 1 (Plk1)-mediated degradation of the APC/C inhibitor, early mitotic...

...Christopher T. Richie; Joshua N. Bembenek; Barry Chestnut; Tokiko Furuta; Jill M. Schumacher; Matthew Wallenfang; Andy Golden Mutations in the Caenorhabditis elegans separase gene, sep-1 , are embryonic lethal. Newly fertilized mutant embryos have defects in polar body extrusion, fail to undergo...

...Nobuaki R. Kudo; Martin Anger; Antoine H. F. M. Peters; Olaf Stemmann; Hans-Christian Theussl; Wolfgang Helmhart; Hiromi Kudo; Christa Heyting; Kim Nasmyth Proteolytic activity of separase is required for chiasma resolution during meiosis I in mouse oocytes. Rec8, the meiosis-specific α-kleisin...

...Rahul Pandey; Stefan Heidmann; Christian F. Lehner Separase cleaves a subunit of the cohesin complex and thereby promotes sister chromatid separation during mitotic and meiotic divisions. Drosophila separase associates with regulatory subunits encoded by the pimples and three rows genes. Three rows...

...Oliver Leismann; Christian F. Lehner Sister chromatid separation during exit from mitosis requires separase. Securin inhibits separase during the cell cycle until metaphase when it is degraded by the anaphase-promoting complex/cyclosome (APC/C). In Drosophila , sister chromatid separation proceeds...