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Keywords: Secretory vesicles
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Journal Articles
J Cell Sci (2013) 126 (11): 2361–2371.
Published: 01 June 2013
... © 2013. Published by The Company of Biologists Ltd 2013 Munc18 SNARE Exocytosis Secretory vesicles Syntaxin Synaptic exocytosis/membrane fusion is highly governed by neuronal Sec1/Munc18 (SM) protein, Munc18-1. The essence of Munc18-1 has been clearly evidenced by strikingly...
Includes: Supplementary data
Journal Articles
J Cell Sci (2006) 119 (13): 2688–2694.
Published: 01 July 2006
... and the fundamental ability to fuse. However, testing for a selective effect of native microdomains on the efficiency of fusion, without affecting membrane cholesterol density, has not been assessed. Hydrolysis of sphingomyelin disrupts native raft domains on secretory vesicles. Disruption of microdomains enriched...
Journal Articles
J Cell Sci (2005) 118 (20): 4833–4848.
Published: 15 October 2005
... steps and requires both protein and lipid membrane components. The role of cholesterol has been investigated using isolated release-ready native cortical secretory vesicles to analyze the Ca 2+ -triggered fusion step of exocytosis. Cholesterol is a major component of vesicle membranes and we show here...
Journal Articles
J Cell Sci (2004) 117 (5): 711–722.
Published: 15 February 2004
... correspond to forming vesicles/granules, and thinning of the surrounding tubules. The tubules eventually break to release the secretory vesicles/granules into the cytoplasm. This process is highly conserved at the morphological level from yeast to mammalian cells. Drs2p, a multi-span transmembrane domain...
Journal Articles
J Cell Sci (2003) 116 (10): 2087–2097.
Published: 15 May 2003
... are their interactions with other proteins manifest? Capitalizing on the differential sensitivity of SNAREs to exogenous proteases, we quantified the selective removal of identified SNAREs from native secretory vesicles without loss of fusion competence. Using previously established fusion assays and a high sensitivity...
Journal Articles
J Cell Sci (1990) 96 (4): 705–710.
Published: 01 August 1990
...DAVID A. BRUMMELL; ANNE CAMIRANDE; GORDON A. MACLACHLAN Rate-zonal centrifugation of pea ( Pisum sativum var. Alaska) stem microsomal membranes on a linear Renografln gradient separated Golgi secretory vesicles from dictyosomes. Secretory vesicles possessed high levels of xyloglucan fucosyl...