.... To this end, we analysed the cytosolic binding partners of Sec61β, a well-defined substrate of TRC40, and identified Bat3 as a previously unknown interacting partner. Depletion of Bat3 inhibits the membrane integration of Sec61β, but not of a second, TRC40-independent, tail-anchored protein, cytochrome b5...

... insertion of TA proteins from recombinant Asna1/TA protein complexes and ER-derived membranes. Our data show that Asna1 can mediate membrane insertion of RAMP4 and Sec61β without the participation of other cytosolic proteins by a mechanism that depends on the presence of ATP or ADP and a protease-sensitive...

... for membrane insertion of three TA proteins, RAMP4, Sec61β and cytocrome b5. We show here that newly synthesised RAMP4 and Sec61β can accumulate in a cytosolic, soluble complex with the ATPase Asna1 before insertion into ER-derived membranes. Membrane insertion of these TA proteins is stimulated by ATP...

... of a multicellular organism. * Author for correspondence (e-mail: mlodzik@embl-heidelberg.de ) 14 09 1999 17 11 1999 © 1999 by Company of Biologists 1999 Drosophila Sec61β Protein translocation Secretion Cuticle Oogenesis Gurken The initial step in the biogenesis...