... of PP2A–B55δ is crucial for controlling entry into and exit from mitosis in Xenopus egg extracts . EMBO J. 28 , 2777 - 2785 . 10.1038/emboj.2009.238 Moreno , S. , Klar , A. and Nurse , P. ( 1991 ). Molecular genetic analysis of fission yeast Schizosaccharomyces pombe...

... complex to the cell cortex in the fission yeast Schizosaccharomyces pombe . Rga6 interacts with the septin complex and partially colocalizes with the septin complex on the cell cortex. Live-cell microscopy analysis further showed septin enrichment at the cortical regions adjacent to the growing cell tip...

...Yu-Chien Chuang; Gerald R. Smith ABSTRACT Appropriate DNA double-strand break (DSB) and crossover distributions are required for proper meiotic chromosome segregation. Schizosaccharomyces pombe linear element proteins (LinEs) determine DSB hotspots; LinE-bound hotspots form three-dimensional...

... -glycosylation. We constructed a platform of 16 Schizosaccharomyces pombe strains that synthesize all possible theoretical combinations of LLOs containing three to zero glucose (Glc) residues and nine to five mannose (Man) residues. The occurrence of unexpected LLOs suggested the requirement of specific Man...

... and canonical centrosomal spindles in fission yeast, which could clarify the mechanisms underlying acentrosomal meiosis. Meiosis Spindle pole body Acentrosomal spindle Schizosaccharomyces pombe Ministerio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 PGC2018-098118...

...Sanju Ashraf; Ye Dee Tay; David A. Kelly; Kenneth E. Sawin ABSTRACT Movement of the cell nucleus typically involves the cytoskeleton and either polymerization-based pushing forces or motor-based pulling forces. In the fission yeast Schizosaccharomyces pombe , nuclear movement and positioning...

...Li Sun; Xiao-Min Liu; Wen-Zhu Li; Yuan-Yuan Yi; Xiangwei He; Yamei Wang; Quan-Wen Jin ABSTRACT In the fission yeast Schizosaccharomyces pombe , both RNAi machinery and RNAi-independent factors mediate transcriptional and posttranscriptional silencing and heterochromatin formation. Here, we show...

...Aleksandar Vještica; Magdalena Marek; Pedro Junior Nkosi; Laura Merlini; Gaowen Liu; Melvin Bérard; Ingrid Billault-Chaumartin; Sophie G. Martin ABSTRACT Schizosaccharomyces pombe is a widely used model organism to study many aspects of eukaryotic cell physiology. Its popularity as an experimental...

... environment to regulate AMPK and TOR signalling. Exposure of fission yeast ( Schizosaccharomyces pombe ) and human cells to external AMP impeded cell growth; however, in yeast this restraining impact required AMPK. In contrast, external ATP rescued the growth defect of yeast mutants with reduced TORC1...

... instability. In this study in fission yeast, we describe a new role for the mitotic inhibitor Wee1 in suppressing replication stress and genome instability. Wee1 Histone H3K36 modification Schizosaccharomyces pombe Synthetic lethality MBF Set2 CDK Replication stress, in which DNA...

... , A. , Tang , E. , Yang , H. and DeCaprio , J. A. ( 1995 ). The Schizosaccharomyces pombe MBF complex requires heterodimerization for entry into S phase . Mol. Cell. Biol.   15 , 2589 - 2599 . 10.1128/MCB.15.5.2589 Ayté , J. , Schweitzer , C. , Zarzov , P. , Nurse , P...

... purified from Schizosaccharomyces pombe cells revealed that the cohesin subunit Psm1 is methylated on two evolutionarily conserved lysine residues, K536 and K1200. We found that mutations that prevent methylation of Psm1 K536 and K1200 render sensitivity to DNA-damaging agents and show positive genetic...

... related fungal organism, the fission yeast Schizosaccharomyces pombe , autophagy of ER and mitochondria is induced by nitrogen starvation and is promoted by three Atg20- and Atg24-family proteins – Atg20, Atg24 and SPBC1711.11 (named here as Atg24b). These proteins localize at the pre-autophagosomal...

... kinases (MAPKK) and MAPKs. The MAPKKK Mkh1 is an initiating kinase in Pmk1 MAPK signaling, which regulates cell integrity in fission yeast ( Schizosaccharomyces pombe ). Our genetic screen for regulators of Pmk1 signaling identified Shk1 kinase binding protein 5 (Skb5), an SH3-domain-containing adaptor...

... protein is prone to aggregate and to be degraded. Tumorigenic mutations might compromise its folding. We identify new factors regulating pVHL folding. VHL Protein aggregation ccRCC Schizosaccharomyces pombe The human VHL gene encodes three different isoforms from two transcripts...

... the stability of the S. pombe CapZ complex to regulate actin function. Rictor TOR TORC2 Myosin II Myosin V Acp1 CAPZA Schizosaccharomyces pombe Target of rapamycin (TOR) signalling plays a key role in modulating the spatial and temporal control of cell growth in response to different...

... and generates mechanical forces on actin filaments . Nat. Commun.   4 , 1883 . 10.1038/ncomms2888 Kanbe , T. , Kobayashi , I. and Tanaka , K. ( 1989 ). Dynamics of cytoplasmic organelles in the cell cycle of the fission yeast Schizosaccharomyces pombe: three-dimensional reconstruction...

...Rebeca Martín-García; Pedro M. Coll; Pilar Pérez ABSTRACT F-BAR domain proteins act as linkers between the cell cortex and cytoskeleton, and are involved in membrane binding and bending. Rga7 is one of the seven F-BAR proteins present in the fission yeast Schizosaccharomyces pombe . In addition...

... trough in a Cdc42 GAP. * Author for correspondence ( sophie.martin@unil.ch ) Competing interests The authors declare no competing interests. 4 09 2013 23 01 2014 © 2014. Published by The Company of Biologists Ltd 2014 Cdc42 Schizosaccharomyces pombe Cell...

... growth in response to extracellular conditions. In Schizosaccharomyces pombe , Rheb and Tsc1–Tsc2 regulate cell cycle progression, the onset of meiosis and the uptake of amino acids. In cells lacking Tsc2 ( Δtsc2 ), the amino acid transporter Aat1, which is normally expressed on the plasma membrane under...