... version of GLUT4 under the control of a muscle-specific promoter. The exofacial HA-tag has enabled fluorescent labelling of only the GLUT4 exposed at the external surface. A distinction between sarcolemma labelling and transverse-tubule labelling has also been possible because the former compartment...

... and to the sarcolemma in striated muscle. We examined the contractile properties and morphology of fast-twitch skeletal muscle from mice lacking keratin 19. Tibialis anterior muscles of keratin-19-null mice showed a small but significant decrease in mean fiber diameter and in the specific force of tetanic contraction...

... ) 11 2 2003 © The Company of Biologists Limited 2003 2003 Costamere Sarcolemma Membrane skeleton C2C12 cells 50MB-1 cells Organized complexes of membrane-associated cytoskeletal proteins (membrane skeletons) maintain the integrity and organization of the plasma membrane...

... of the Na,K-ATPase colocalize with β-spectrin and ankyrin 3 in costameres, structures at the sarcolemma that lie over Z and M-lines and in longitudinal strands. In immunoprecipitates, the α1 and α2 subunits of the Na,K-ATPase as well as ankyrin 3 associate with β-spectrin/α-fodrin heteromers and with a pool...

... osmotic shock cytochalasin D sarcolemma microfilament system To localize dystrophin, indirect immunofluorescence was performed on cryosections of cultured myotubes. For this purpose, cultures were detached by trypsin treatment and harvested by centrifugation at 200 g in conical plastic tubes...

... or myofibres in vivo with recombinant genes encoding human dystrophin deletion mutants shows, however, that not only the C terminus of dystrophin but also its N-terminal actin-binding domain can locate independently to the muscle sarcolemma. This observation suggests that lack of sarcolemma-associated...